Genetics Chapter on Mutations & Biotechnology

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Questions and Answers

What was the critical observation that led Archibald Garrod to investigate alkaptonuria?

  • The genetic mutation in genes causing alkapton build-up.
  • The lack of enzymes to catalyze reactions in alkapton metabolism.
  • The patients' inability to synthesize alkapton.
  • The black color of the urine of the patients when exposed to air. (correct)

Which of the following accurately describes the experimental setup used by Beadle and Tatum using Neurospora crassa?

  • They introduced specific genes and observed the new characteristics of the mutant mould.
  • They exposed the mould to x-rays, and observed the mutations that resulted in the inability to grow on a minimal media.
  • They grew the mould in a rich media and observed the mutated offspring.
  • They created mutations in the mould and observed which additional nutrients were required for growth on a minimal media. (correct)

The initial 'one gene-one enzyme hypothesis' as proposed by Beadle and Tatum was later modified because:

  • not all genes code for polypeptides.
  • some proteins are composed of multiple different polypeptides
  • some enzymes are not encoded by a specific gene
  • not all proteins function as enzymes. (correct)

In the context of the central dogma, what is the role of RNA?

<p>To act as an intermediary molecule between DNA and protein synthesis. (C)</p> Signup and view all the answers

Which scientific contribution is incorrectly matched with the scientist?

<p>Edward Tatum - proposed that genes code for enzymes. (D)</p> Signup and view all the answers

According to the provided text, which chapter and section primarily covers the topic of genetic mutations?

<p>Chapter 7.5, pages 340-345 (C)</p> Signup and view all the answers

Which of the following is the correct textbook reference for the topic of biotechnology which includes manipulating and cloning DNA?

<p>Pages 366-375, 376-385, and 386-390 (A)</p> Signup and view all the answers

Which textbook pages are specifically assigned for practice problems related to the topic of DNA sequencing?

<p>Page 385 (#1, 2) (C)</p> Signup and view all the answers

Based on the textbook assignments, which of the following page numbers does NOT have associated practice questions?

<p>Page 377 (B)</p> Signup and view all the answers

According to the provided text, which listed chapter includes a section on eukaryotes?

<p>No chapter explicitly lists eukaryotes (B)</p> Signup and view all the answers

What is the primary role of transfer RNA (tRNA) in translation?

<p>To carry specific amino acids to the ribosome based on mRNA codons. (B)</p> Signup and view all the answers

Which of the following is a key structural feature of tRNA that enables it to perform its function?

<p>A single-stranded molecule with an anticodon loop and an amino acid attachment site. (C)</p> Signup and view all the answers

What analogy best represents the relationship between codons and amino acids?

<p>A lock and key, where each codon is the lock and each amino acid is the key. (A)</p> Signup and view all the answers

If a mRNA sequence reads 5’-CCG-3’, which tRNA anticodon would correctly bind to it?

<p>3’-GGC-5’ (B)</p> Signup and view all the answers

Why is the start codon, AUG, particularly important for translation?

<p>It signals the ribosome to begin protein synthesis and specifies methionine. (B)</p> Signup and view all the answers

During translation, what is the primary function of the A site on the ribosome?

<p>To accept a tRNA molecule whose anticodon is complementary to the mRNA codon. (A)</p> Signup and view all the answers

Which of the following best describes the role of RNA polymerase during gene expression?

<p>Creating a complementary RNA sequence from a DNA template during transcription. (A)</p> Signup and view all the answers

If a mutation occurs in a single base within a mRNA sequence, what would be the most likely consequence during protein synthesis?

<p>The mutation could result in the incorporation of a different amino acid into the polypeptide chain. (A)</p> Signup and view all the answers

Why is it essential to have a stop codon during the process of translation?

<p>To tell the ribosome when protein synthesis is complete, ending polypeptide chain elongation. (C)</p> Signup and view all the answers

Considering that all individuals are made up of the same four nucleotides in DNA and RNA, and the same twenty amino acids in our proteins, what accounts for the vast diversity between individuals?

<p>Variations in the specific sequences of nucleotides in DNA, leading to differences in RNA and protein sequences. (B)</p> Signup and view all the answers

Flashcards

Genetic Mutations

A change in the DNA sequence of an organism.

Eukaryotes

Organisms with membrane-bound organelles, such as the nucleus.

DNA Sequencing

The process of determining the sequence of nucleotides in a DNA molecule.

Biotechnology

The application of biological techniques to create useful products.

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Manipulating and Cloning DNA

The deliberate manipulation of genes for various purposes, such as creating new drugs or crops.

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Alkaptonuria

A condition where the body cannot break down alkapton, leading to a build-up that causes the urine to turn black when exposed to air.

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One Gene-One Enzyme Hypothesis

A hypothesis that a single gene is responsible for coding for a single enzyme.

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Neurospora crassa

Simple organisms that could grow on minimal media containing only a few basic substances.

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Mutations in Neurospora crassa

Changes in DNA sequence brought about by X-ray exposure in Beadle & Tatum's experiments.

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Central Dogma

The flow of genetic information from DNA to RNA and then to protein; it encompasses two major steps: transcription and translation.

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Transcription

The process by which genetic information encoded in DNA is copied into a messenger RNA (mRNA) molecule.

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Translation

The process by which the genetic code in mRNA is translated into a sequence of amino acids, forming a protein.

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Codon

A sequence of three nucleotides in mRNA that codes for a specific amino acid.

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Anticodon

A sequence of three nucleotides in tRNA that is complementary to a codon in mRNA.

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Messenger RNA (mRNA)

Ribonucleic acid (RNA) that carries genetic information from DNA to ribosomes, where protein synthesis takes place.

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What is a codon?

A codon consists of three consecutive nucleotide bases within mRNA that specifies a particular amino acid in a polypeptide chain. For example, the codon AUG codes for the amino acid methionine. Multiple codons can code for the same amino acid, providing redundancy in the genetic code.

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What is the start codon?

The start codon is the first codon in an mRNA sequence, typically AUG, which signals the beginning of translation and specifies the amino acid methionine. It signifies the start of protein synthesis.

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How does a stop codon function in translation?

A stop codon marks the end of protein synthesis. It signals the ribosome to stop translating the mRNA sequence, terminating the polypeptide chain.

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What is the role of tRNA in translation?

Transfer RNA (tRNA) is a type of RNA molecule that carries specific amino acids to ribosomes during protein synthesis. It has an anticodon loop containing three bases complementary to the mRNA codon, ensuring the correct amino acid is added to the polypeptide chain.

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What is the significance of an anticodon on a tRNA molecule?

The anticodon region of a tRNA molecule is a sequence of three nucleotides that is complementary to a specific codon on mRNA. This allows the tRNA to base-pair with the mRNA, ensuring the correct amino acid is incorporated into the growing polypeptide chain.

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