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Questions and Answers
What is the purpose of adding dideoxynucleotides in Sanger's sequencing method?
What is the purpose of adding dideoxynucleotides in Sanger's sequencing method?
- To terminate DNA synthesis (correct)
- To separate DNA fragments using electrophoresis
- To label different fluorescent dyes
- To initiate DNA synthesis
What is the difference between a gene and an allele?
What is the difference between a gene and an allele?
- A gene is a variant form of an allele
- An allele is responsible for the synthesis of DNA strands
- An allele is a variant form of a gene (correct)
- A gene is responsible for eye color, while an allele is responsible for genetic mutations
What is the advantage of using DNA sequencing over other methods for mutation detection?
What is the advantage of using DNA sequencing over other methods for mutation detection?
- It requires a smaller amount of DNA sample
- It is only used for specific types of mutations
- It is faster and cheaper than other methods
- It can detect unknown mutations (correct)
Which of the following is true about Sanger's sequencing method?
Which of the following is true about Sanger's sequencing method?
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What is the purpose of adding dideoxynucleotides to the reaction tube in Sanger's sequencing method?
What is the purpose of adding dideoxynucleotides to the reaction tube in Sanger's sequencing method?
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What is the significance of the different bands obtained from electrophoresis in Sanger's sequencing method?
What is the significance of the different bands obtained from electrophoresis in Sanger's sequencing method?
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