Podcast
Questions and Answers
Which enzymes were essential for genetic engineering or recombinant DNA technology?
Which enzymes were essential for genetic engineering or recombinant DNA technology?
- Polymerase enzymes, helicase enzymes, ligases
- Polymerase enzymes, ligases, vectors
- Restriction enzymes, helicase enzymes, vectors
- Restriction enzymes, polymerase enzymes, ligases (correct)
What is the function of the first restriction endonuclease, Hind II?
What is the function of the first restriction endonuclease, Hind II?
- It enhances the functioning of polymerase enzymes in genetic engineering
- It adds methyl groups to DNA to restrict the growth of bacteriophage
- It isolates and characterizes specific DNA nucleotide sequences
- It cuts DNA at a specific point by recognizing a particular sequence of six base pairs (correct)
What was the role of the first isolated restriction endonuclease, Hind II?
What was the role of the first isolated restriction endonuclease, Hind II?
- Isolating and characterizing specific DNA nucleotide sequences
- Enhancing the functioning of ligases in genetic engineering
- Adding methyl groups to DNA to restrict the growth of bacteriophage
- Cutting DNA at a specific point by recognizing a particular sequence of six base pairs (correct)
What did the two enzymes isolated in 1963, responsible for restricting the growth of bacteriophage in Escherichia coli, do?
What did the two enzymes isolated in 1963, responsible for restricting the growth of bacteriophage in Escherichia coli, do?
What is the specific base sequence recognized by Hind II for cutting DNA?
What is the specific base sequence recognized by Hind II for cutting DNA?