Genetic Engineering Tools Quiz
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Questions and Answers

Which enzymes were essential for genetic engineering or recombinant DNA technology?

  • Polymerase enzymes, helicase enzymes, ligases
  • Polymerase enzymes, ligases, vectors
  • Restriction enzymes, helicase enzymes, vectors
  • Restriction enzymes, polymerase enzymes, ligases (correct)

What is the function of the first restriction endonuclease, Hind II?

  • It enhances the functioning of polymerase enzymes in genetic engineering
  • It adds methyl groups to DNA to restrict the growth of bacteriophage
  • It isolates and characterizes specific DNA nucleotide sequences
  • It cuts DNA at a specific point by recognizing a particular sequence of six base pairs (correct)

What was the role of the first isolated restriction endonuclease, Hind II?

  • Isolating and characterizing specific DNA nucleotide sequences
  • Enhancing the functioning of ligases in genetic engineering
  • Adding methyl groups to DNA to restrict the growth of bacteriophage
  • Cutting DNA at a specific point by recognizing a particular sequence of six base pairs (correct)

What did the two enzymes isolated in 1963, responsible for restricting the growth of bacteriophage in Escherichia coli, do?

<p>One added methyl groups to DNA, while the other cut DNA (B)</p> Signup and view all the answers

What is the specific base sequence recognized by Hind II for cutting DNA?

<p>A particular sequence of six base pairs (C)</p> Signup and view all the answers

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