Genetic Engineering Overview
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What is the initial step in the DNA modification process?

  • DNA is extracted from cells. (correct)
  • DNA is cut into smaller pieces.
  • Restriction enzymes are applied.
  • DNA is combined with DNA from another organism.
  • What term describes the function of a gene?

  • A cellular structure that synthesizes RNA.
  • A sequence of DNA that codes for a protein. (correct)
  • A sequence of RNA that regulates other genes.
  • A type of enzyme that modifies proteins.
  • What do we call the DNA that comes from two sources?

  • Polygeneic DNA.
  • Haploidy DNA.
  • Eukaryotic DNA.
  • Transgenic DNA. (correct)
  • What is the role of restriction enzymes in DNA modification?

    <p>To cut specific sequences of DNA. (A)</p> Signup and view all the answers

    Which of the following best describes the process of direct replacement of genes?

    <p>Recombination of existing genes. (A)</p> Signup and view all the answers

    In the context of DNA modification, what is mutation?

    <p>The alteration of existing genes. (A)</p> Signup and view all the answers

    Why are restriction enzymes referred to as 'scissor-like' enzymes?

    <p>They specifically cut DNA at certain sequences. (D)</p> Signup and view all the answers

    What is usually performed after DNA is cut into smaller pieces?

    <p>DNA is combined with the DNA of another organism. (C)</p> Signup and view all the answers

    What is the main purpose of genetic engineering?

    <p>To directly modify an organism's genome (D)</p> Signup and view all the answers

    What are organisms modified through genetic engineering commonly referred to as?

    <p>Genetically modified organisms (GMOs) (C)</p> Signup and view all the answers

    In what year was the first genetically modified bacteria created?

    <p>1973 (B)</p> Signup and view all the answers

    Which genetically modified organism was first sold commercially?

    <p>Insulin-producing bacteria (B)</p> Signup and view all the answers

    Which of the following years marks the introduction of genetically modified food to the market?

    <p>1994 (D)</p> Signup and view all the answers

    What type of genetically modified organism was introduced in 2003?

    <p>Glofish (B)</p> Signup and view all the answers

    Which genetic modification is NOT mentioned in the content as an early development?

    <p>GM corn (D)</p> Signup and view all the answers

    What is the main component of an organism's genome that genetic engineering targets?

    <p>Specific traits (genes) (D)</p> Signup and view all the answers

    What is the primary function of a restriction enzyme in genetic engineering?

    <p>Cutting or cleaving DNA (C)</p> Signup and view all the answers

    What are sticky ends in the context of DNA?

    <p>Ends of DNA that allow for specific pairing with complementary strands (A)</p> Signup and view all the answers

    What role does DNA ligase play in genetic engineering?

    <p>It facilitates the insertion of genes into plasmids (D)</p> Signup and view all the answers

    What genetic modification has been made to cabbage?

    <p>Venomous scorpion genes (A)</p> Signup and view all the answers

    After constructing a recombinant plasmid, what is the next step in genetic engineering?

    <p>Insert the recombinant plasmid back into a bacterial cell (C)</p> Signup and view all the answers

    Which of the following is NOT a reason for genetically modifying crops?

    <p>Faster spoilage (C)</p> Signup and view all the answers

    Which statement best describes the action of restriction enzymes in plasmid manipulation?

    <p>They cleave the DNA at specific sequences (C)</p> Signup and view all the answers

    Which of the following best describes the characteristic of sticky ends?

    <p>They facilitate the formation of recombinant DNA by allowing complementary base pairing (C)</p> Signup and view all the answers

    What is the result of genetic modification in fast-growing salmon?

    <p>Continuous growth hormone production (D)</p> Signup and view all the answers

    Which enzyme is primarily responsible for the joining of DNA fragments?

    <p>DNA ligase (B)</p> Signup and view all the answers

    How has genetic modification affected methane production in cattle?

    <p>25% less methane production (D)</p> Signup and view all the answers

    What does bioluminescent imaging (BLI) primarily detect?

    <p>Different types of cells (A)</p> Signup and view all the answers

    What is the end goal after inserting a gene of interest into a plasmid?

    <p>To produce multiple copies of a gene in a bacterial cell (B)</p> Signup and view all the answers

    Which is a concern regarding genetically modified organisms (GMOs)?

    <p>Potential ecological impacts (C)</p> Signup and view all the answers

    What benefit does the genetic engineering of crops NOT aim to achieve?

    <p>Increased allergenic properties (A)</p> Signup and view all the answers

    What type of animals can be modified for novelty uses?

    <p>Glofish (C)</p> Signup and view all the answers

    What is the primary reason bacteria are commonly used in genetic engineering?

    <p>Their simple structure allows for easy manipulation of DNA. (B)</p> Signup and view all the answers

    What role does a restriction enzyme play in genetic engineering?

    <p>It cuts or cleaves the DNA and plasmid. (A)</p> Signup and view all the answers

    What is the purpose of DNA Ligase in genetic engineering?

    <p>To join the gene of interest to the plasmid. (A)</p> Signup and view all the answers

    Which process follows the insertion of the recombinant plasmid back into the bacterial cell?

    <p>Isolation of the gene copies. (D)</p> Signup and view all the answers

    What products have been derived from genetically modified cyanobacteria?

    <p>Hydrocarbons such as plastics and fuels. (A)</p> Signup and view all the answers

    What is a key benefit of using transgenic bacteria?

    <p>They can produce essential products like insulin. (A)</p> Signup and view all the answers

    How do banana vaccines work according to the content?

    <p>By injecting a modified virus into banana saplings. (A)</p> Signup and view all the answers

    What is the final outcome of inserting a gene of interest into another organism?

    <p>It will confer desired traits to the organism. (A)</p> Signup and view all the answers

    What is the primary role of restriction enzymes in genetic engineering?

    <p>To cut specific sequences in DNA (A)</p> Signup and view all the answers

    What is meant by the term 'plasmid' in the context of DNA modification?

    <p>A small circular DNA molecule in bacteria (C)</p> Signup and view all the answers

    Which step follows after the DNA segments are cut by restriction enzymes?

    <p>Insertion of recombinant DNA into a living cell (C)</p> Signup and view all the answers

    In genetic engineering, what is the purpose of gene splicing?

    <p>To attach DNA segments from one organism to another (C)</p> Signup and view all the answers

    What is the function of DNA ligase in recombinant DNA technology?

    <p>To splice DNA segments together (B)</p> Signup and view all the answers

    What characteristic distinguishes plasmids from chromosomal DNA in bacteria?

    <p>Plasmids replicate independently from chromosomal DNA (C)</p> Signup and view all the answers

    What is the 'gene of interest' in the context of recombinant DNA?

    <p>A gene chosen for modification or study (D)</p> Signup and view all the answers

    What does it mean when it is said that plasmids act as vectors in genetic engineering?

    <p>They transport and deliver genes into host cells (D)</p> Signup and view all the answers

    Flashcards

    What is genetic engineering?

    Genetic engineering is the process of directly altering an organism's genetic makeup, specifically its DNA, to modify specific traits or genes.

    What are GMOs?

    Genetically Modified Organisms (GMOs) are organisms that have been created using genetic engineering techniques.

    When were the first GMOs created?

    The first genetically modified bacteria were created in 1973.

    When were the first genetically modified mice created?

    In 1974, genetically modified mice were created, marking a significant step in the development of GMOs.

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    When was the first commercial GMO developed?

    In 1982, the first commercial development of GMOs occurred with the creation of insulin-producing bacteria.

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    When was the first GMO food sold?

    The first genetically modified food, the Flavr-Savr tomato, was introduced to the market in 1994.

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    When were GMOs first sold as pets?

    In 2003, GMOs began to be sold as pets, with the example of Glofish, a genetically modified fish.

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    What is the process of creating GMOs?

    The process of creating GMOs involves modifying the organism's genetic material, typically through techniques like gene insertion or gene editing.

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    What is a gene?

    A gene is a segment of DNA that contains the code for making a specific protein.

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    What is a GMO?

    GMO stands for Genetically Modified Organism. GMOs are organisms whose genetic material has been altered in a way that doesn't occur naturally.

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    What is the GMO process?

    The process of creating a GMO involves altering the organism's DNA. This can be done by inserting new genetic material, replacing existing genes, removing genes, or mutating existing genes.

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    What are Restriction Enzymes?

    Restriction enzymes are like molecular scissors that cut DNA at specific sequences.

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    What is Recombinant DNA?

    Recombinant DNA is a combination of DNA from two or more different sources.

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    How is DNA modified? Step 1

    The first step in modifying DNA is to extract it from cells.

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    How is DNA modified? Step 2

    The second step in modifying DNA is to cut it into smaller pieces using restriction enzymes.

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    How is DNA modified? Step 3

    The third step in modifying DNA is to combine the cut DNA with DNA from another organism.

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    What is gene splicing?

    The process of attaching pieces of DNA segments to the DNA of other organisms.

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    What is a plasmid?

    A small circular DNA molecule found in bacteria and some other microscopic organisms.

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    What is a restriction enzyme?

    A restriction enzyme is a "scissor-like" enzyme that cuts specific sequences in the DNA. It plays a role in genetic engineering.

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    What is the final step in creating recombinant DNA?

    The combined DNA is placed inside a living cell, allowing it to replicate and express the new genetic information.

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    How is recombinant DNA used?

    The new DNA, called recombinant DNA, is introduced into a living cell.

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    What are the benefits of genetic engineering?

    The process of genetic engineering can be used to produce useful products such as medicines and food.

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    Restriction enzyme

    A specific sequence of DNA that recognizes and cuts DNA at a particular site. It's like a pair of molecular scissors for DNA.

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    Sticky Ends

    Short stretches of single-stranded DNA at the end of a DNA molecule, usually created by restriction enzymes. They act like tiny 'sticky notes' for DNA.

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    DNA Ligase

    An enzyme that joins two DNA fragments together. It's like a molecular glue for DNA.

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    Recombinant Plasmid

    A DNA molecule that has been altered by the insertion of a foreign gene.

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    Transformation

    The process of introducing a recombinant plasmid into a bacterial cell.

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    Gene Cloning

    The creation of multiple copies of a specific gene or DNA sequence within a host cell.

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    Genetically Modified Organism (GMO)

    A modified organism that contains genetic material from another organism. They often have beneficial traits, like increased crop yield or disease resistance.

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    Genetic Engineering

    The process of altering an organism's genetic makeup to modify its traits.

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    Why are bacteria commonly used for genetic engineering?

    Bacteria are the most common GMOs because their simple structure permits easy manipulation of their DNA.

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    What is an interesting application of GMO bacteria?

    The use of genetically modified bacteria to produce hydrocarbons (plastics and fuels) that were previously found only in fossil fuels.

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    What is gene transfer?

    The process of isolating a specific gene from one organism and inserting it into another organism's DNA.

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    What does DNA ligase do?

    The process of joining DNA fragments together, used in gene transfer to link the desired gene to a plasmid.

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    What are some benefits of genetically modified crops?

    Genetically modified crops are engineered to have beneficial traits, such as resistance to insects, herbicides, disease, drought, freeze, and improved yields.

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    How could genetic engineering be used to modify animals?

    Genetically modified animals could be used to improve livestock production, control diseases, or even create novel animal products.

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    What are bioluminescent animals?

    Bioluminescent animals, like Glofish, are genetically modified to produce light. This is possible by inserting genes that code for light-emitting proteins.

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    What is bioluminescent imaging (BLI)?

    Bioluminescent imaging (BLI) is a technique that uses light-emitting proteins to track biological processes in living organisms. This can be helpful for studying diseases and the development of new therapies.

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    What are fast-growing salmon?

    Fast-growing salmon are genetically modified to produce growth hormones continuously. This results in fish that grow faster than wild salmon, but there are concerns about potential environmental and food safety issues.

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    What are less smelly cows?

    Less smelly cows are genetically modified to reduce methane production. Methane is a powerful greenhouse gas, and this modification could help reduce the environmental impact of cattle farming.

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    What are some concerns regarding genetically modified foods?

    One concern about genetically modified foods is whether they are safe for human consumption. There are also concerns about the impact of GMOs on the environment, including the potential for gene flow to wild populations.

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    What are biotechniques?

    Biotechniques are the methods and tools used in genetic engineering. These techniques include gene cloning, gene editing, and DNA sequencing.

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    Study Notes

    Genetic Engineering Overview

    • Genetic engineering is the direct modification of an organism's genome, altering its traits.
    • Genetically modified organisms (GMOs) are created through genetic engineering.

    History of GMO Development

    • 1973: First genetically modified bacteria were created.
    • 1974: Genetically modified mice were created.
    • 1982: First commercial GMOs (insulin-producing bacteria) were developed.
    • 1994: Genetically modified food (Flavr Savr tomatoes) became available.
    • 2003: Genetically modified pets (GloFish) became available.

    GMO Process

    • Step 1: Extract DNA from cells.
    • Step 2: Cut the extracted DNA into smaller pieces using restriction enzymes.
    • Step 3: Combine the cut DNA with DNA from another organism.
    • Step 4: Insert the combined DNA (recombinant DNA) into a living cell.

    Gene Splicing

    • Gene splicing is a process where scientists attach DNA segments from different organisms to existing DNA.

    Recombinant DNA

    • Recombinant DNA is DNA that has been combined from two or more sources.

    What Recombinant DNA Does

    • The recombinant DNA directs the production of proteins/traits from the new source.

    GMO Concerns

    • Potential risk to human health from consuming GMOs.
    • Environmental and wildlife concerns related to GMOs.
    • Increased pesticide/herbicide use may be a concern.
    • Impact on farmer's health.
    • Seed/pollen drift.

    BioTechniques

    • Biolistics: A technique using a "gene gun" to insert DNA into plant cells.

    Engineering Animals

    • Genetic engineering could solve problems by modifying animals.

    Other GMO Applications

    • Transgenic Bacteria: Bacteria modified to produce human-needed substances like insulin or clotting factors.
    • Banana Vaccines: Bananas modified to contain virus proteins.
    • Venomous Cabbage: Cabbages that are resistant to insects.
    • Bioluminescent Animals: Animals that glow, useful for tracking proteins and identifying cells.
    • Fast-Growing Salmon: Salmon with genes from other fish that promote faster growth.
    • Less Smelly Cows (Methane Reduction): Modifying cattle to produce less methane.

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    Description

    This quiz covers the fundamentals of genetic engineering, from the history of GMO development to the processes involved in creating GMOs. Learn about the techniques used for gene splicing and recombinant DNA technology. Perfect for students looking to understand modern genetic manipulation.

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