Genetic Engineering and Biotechnology

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Questions and Answers

What is the primary source of Taq polymerase, an enzyme used in PCR?

  • Molecular scissors
  • Ligase-producing organism
  • Heat-resistant virus
  • Thermophilic bacteria (correct)

What is the purpose of ligase in molecular cloning?

  • To visualize DNA
  • To cut DNA molecules
  • To prevent self-ligation
  • To repair the phosphodiester backbone of DNA (correct)

What is the primary function of molecular scissors in genetic engineering?

  • To visualize DNA
  • To cut DNA molecules (correct)
  • To prevent self-ligation
  • To join DNA molecules

Which stage of PCR requires a high temperature?

<p>Denaturing (B)</p> Signup and view all the answers

What is the potential benefit of genetically modified crops?

<p>To enhance all forms of human agriculture (D)</p> Signup and view all the answers

What is the purpose of phosphatase in genetic engineering?

<p>To prevent self-ligation (C)</p> Signup and view all the answers

What is the primary goal of gene therapy?

<p>To cure hereditary diseases (C)</p> Signup and view all the answers

What is the significance of E. coli in biotechnology?

<p>It produces recombinant insulin (C)</p> Signup and view all the answers

What is the potential risk of genetic engineering in agricultural biotechnology?

<p>It can lead to the flow of genes from modified plants to natural plants, threatening biodiversity (C)</p> Signup and view all the answers

What is the primary function of the pancreas?

<p>To secrete insulin (C)</p> Signup and view all the answers

What is the ethical principle demonstrated by the doctor in the hospital scenario?

<p>Beneficence (B)</p> Signup and view all the answers

What is the bioethical principle demonstrated by the statement about the environment?

<p>Environmental ethics (C)</p> Signup and view all the answers

What is the primary concern associated with the use of terminator technology?

<p>It could lead to the killing of natural populations through outcrossing (C)</p> Signup and view all the answers

What is the primary purpose of bioreactors?

<p>To provide optimal conditions for cell growth and chemical production (B)</p> Signup and view all the answers

What is the main goal of gene therapy?

<p>To cure genetic defects in embryos (A)</p> Signup and view all the answers

What is the benefit of Golden Rice?

<p>It is rich in beta-carotene (B)</p> Signup and view all the answers

What is the first stage of gene therapy?

<p>Lymphocytes culture outside the body (B)</p> Signup and view all the answers

What is an alternative method to cure Adenosine deaminase deficiency?

<p>Bone marrow transplantation (C)</p> Signup and view all the answers

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