14 Questions
What is the purpose of a gene 'probe' in molecular biology?
To locate and bind to its complementary sequence
In which technique is RNA separated by gel electrophoresis and transferred onto a membrane sheet?
Northern blotting
What is the function of polymerase chain reaction (PCR) in molecular biology?
To amplify minute amounts of DNA
What are the 'oligonucleotide primers' used for in polymerase chain reaction (PCR)?
To initiate the amplification process
What is the purpose of in situ hybridization in molecular biology?
To localize native nucleic acid sequences within the cell
How does a DNA probe differ from oligonucleotide primers in molecular biology?
A DNA probe is used to locate and bind to its complementary sequence, while oligonucleotide primers amplify minute amounts of DNA
What is the purpose of denaturing the genomic DNA in the polymerase chain reaction (PCR)?
To convert it into single-stranded DNA
What happens after the primers bind to their target DNA in the polymerase chain reaction (PCR)?
The DNA polymerase extends the primers in opposite directions
What is the purpose of real-time polymerase chain reaction (QRT-PCR)?
To simultaneously quantify and amplify a given DNA sequence
What technique can be combined with real-time polymerase chain reaction to quantify low abundance messenger RNA (mRNA)?
Reverse transcription polymerase chain reaction
What is the purpose of using fluorescent dyes in real-time polymerase chain reaction (QRT-PCR)?
To quantify the DNA after each round of amplification
What is the function of modified DNA oligonucleotide probes in real-time polymerase chain reaction (QRT-PCR)?
To fluoresce when hybridized with complementary DNA
What does real-time polymerase chain reaction (QRT-PCR) allow researchers to determine?
The presence and number of copies of a specific sequence in a sample
What is the purpose of cycle repetition in polymerase chain reaction (PCR)?
To produce millions of amplifications
Explore the relationship between genetic disorders and DNA probes in molecular cell biology. Learn how DNA probes with complementary base sequences can be used to identify specific gene positions or presence.
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