Genetic Code and Translation Quiz
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Questions and Answers

What role do aminoacyl-tRNA synthetases perform in protein synthesis?

  • They synthesize RNA from DNA templates.
  • They add amino acids to the growing polypeptide chain.
  • They catalyze the attachment of specific amino acids to their corresponding tRNA. (correct)
  • They regulate the initiation of transcription.
  • Which sequence is recognized to initiate translation in prokaryotes?

  • The Kozak sequence
  • The G-cap
  • The TATA box
  • The Shine-Dalgarno sequence (correct)
  • During eukaryotic translation initiation, what is the function of the G-cap?

  • It determines the stop codon for translation.
  • It signals the start of transcription.
  • It protects the mRNA from degradation and aids in ribosome binding. (correct)
  • It is the site for tRNA attachment.
  • Which proteins are involved in the elongation step of translation?

    <p>EF-TU, peptidyl transferase, and EF-G (B)</p> Signup and view all the answers

    What are stop codons responsible for during translation termination?

    <p>Signaling the end of translation and the release of the polypeptide chain. (D)</p> Signup and view all the answers

    What is the main reason the lac operon is only expressed under specific conditions?

    <p>Lactose is absent during glucose metabolism. (B)</p> Signup and view all the answers

    Which of the following describes a classic histone remodeling complex's role in transcription?

    <p>It alters chromatin structure to make DNA more accessible for transcription. (A)</p> Signup and view all the answers

    Which statement correctly differentiates between constitutive and regulated gene expression?

    <p>Regulated expression can respond to environmental signals, while constitutive expression is always active. (D)</p> Signup and view all the answers

    What influences the probability of a mutation being classified as cis-acting versus trans-acting?

    <p>The location of the mutation relative to the gene it influences. (C)</p> Signup and view all the answers

    What role do enhancers play in gene regulation?

    <p>They facilitate the recruitment of transcription factors and enhance the rate of transcription. (C)</p> Signup and view all the answers

    What is the primary DNA modification that regulates gene expression in eukaryotes and what effect does it typically have on transcription?

    <p>Methylation; negative effect (C)</p> Signup and view all the answers

    How does alternative splicing influence gene regulation?

    <p>It creates different mRNA transcripts from the same gene. (A)</p> Signup and view all the answers

    What is RNA interference (RNAi) and how does it affect transcription in eukaryotes?

    <p>A silencing mechanism that can downregulate gene expression. (D)</p> Signup and view all the answers

    What differentiates a missense mutation from a nonsense mutation?

    <p>Missense changes one amino acid, nonsense creates a premature stop codon. (B)</p> Signup and view all the answers

    Which type of mutation can result from spontaneous tautomerization of bases?

    <p>Substitution mutations (C)</p> Signup and view all the answers

    What is the role of proofreading by DNA polymerase?

    <p>To ensure accurate nucleotide pairing during DNA synthesis. (B)</p> Signup and view all the answers

    How does UV light cause damage to DNA, and what repair mechanism can reverse such damage?

    <p>It forms thymine dimers; nucleotide excision repair can fix it. (C)</p> Signup and view all the answers

    Which mutation type is characterized by a change in the DNA sequence that cannot revert back to the original?

    <p>Forward mutation (B)</p> Signup and view all the answers

    What are restriction endonucleases and what do they do?

    <p>Enzymes that cut DNA at specific sequences. (B)</p> Signup and view all the answers

    What barrier must be overcome when expressing mammalian genes in prokaryotic cells?

    <p>The lack of post-translational modifications in prokaryotes. (A)</p> Signup and view all the answers

    Flashcards

    DNA modification for gene regulation

    Methylation of DNA is a common DNA modification, affecting gene expression by influencing whether transcription occurs.

    Alternative splicing effect on gene regulation

    Alternative splicing creates different mRNA versions from one gene, leading to different protein products, regulating gene expression.

    RNA interference (RNAi)

    RNAi is a gene silencing process that reduces expression of specific mRNAs.

    Point mutation types

    Point mutations include transitions (purine to purine or pyrimidine to pyrimidine) and transversions (purine to pyrimidine or vice versa).

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    Silent mutation

    A silent mutation changes a codon but does not alter the amino acid sequence of the protein.

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    Spontaneous mutation

    Spontaneous mutations occur naturally without external causes. Common causes include tautomerization and errors during DNA replication.

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    DNA polymerase proofreading

    DNA polymerase has an ability to check for errors after making a base-pair, fixing errors before next base-pair is created.

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    UV light DNA damage

    UV light causes thymine dimers, which distort DNA.

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    Molecular cloning steps

    Molecular cloning involves isolating DNA, inserting it into a vector, and then using a host cell to copy the DNA.

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    Mammalian gene prokaryotic expression barriers

    Mammalian genes cannot be directly expressed in prokaryotes due to differences in genetic regulatory mechanisms.

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    Codons in translation

    A sequence of three nucleotides that codes for a specific amino acid in protein synthesis.

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    tRNA structure

    Transfer RNA carries amino acids to the ribosome during protein synthesis. It has a specific structure with an anticodon that pairs with mRNA.

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    Aminoacyl-tRNA synthetases

    Enzymes that attach the correct amino acid to its corresponding tRNA.

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    Base Pairing (tRNA-mRNA)

    tRNA's anticodon pairs with mRNA's codon according to established base pairing rules.

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    Prokaryotic translation initiation

    Involves the Shine-Dalgarno sequence on mRNA binding to the ribosome.

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    Eukaryotic translation initiation

    Involves the ribosome binding to the 5' cap of mRNA, unlike prokaryotes.

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    Translation elongation (prokaryotes)

    Process of adding amino acids to the growing polypeptide chain.

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    Translation termination

    Stop codons signal the end of protein synthesis.

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    Constitutive gene expression

    Genes expressed continuously and at a stable rate.

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    Gene regulation by activators

    Bind to DNA to enhance transcription of specific genes.

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    Study Notes

    Genetic Code and Translation

    • Understand how to read a table of the genetic code
    • Understand codons and how they are read
    • Recognize tRNA parts
    • Predict the amino acid a tRNA carries based on its sequence
    • Function of aminoacyl tRNA synthetases
    • How base pairing occurs between tRNA and mRNA
    • Base pairing rules and wobble rules
    • Translation initiation in prokaryotes (step-by-step)
    • Shine-Dalgarno sequence function
    • Translation initiation in eukaryotes (step-by-step)
    • Role of G-cap
    • Steps of translation elongation (prokaryotic)
    • Roles of EF-Tu, peptidyl transferase, and EF-G
    • A and P sites of the ribosome
    • Steps of translation termination (prokaryotic)
    • Role of release factors
    • Stop codons
    • Protein trafficking in eukaryotes

    Gene Expression Regulation

    • Constitutive vs. regulated gene expression
    • Timing of gene regulation
    • Functions of activators, repressors, inhibitors, corepressors, and inducers
    • Example: lac operon expression conditions
    • Cis-acting vs. trans-acting mutations
    • Classification of mutations
    • Transcriptional regulation using enhancer, silencer, and insulator sequences
    • Classic histone modifying proteins and chromatin remodeling
    • Steps for making DNA available for transcription in eukaryotes
    • DNA modification affecting transcription in eukaryotes

    Mutations and DNA Repair

    • Alternative splicing's effect on gene regulation
    • RNA interference mechanism and effect on eukaryotes
    • Point mutations (silent, missense, nonsense, frameshift)
    • Transition and transversion mutations
    • Mutations in noncoding DNA
    • Forward, reverse, reversion, and suppressor mutations
    • Spontaneous mutations
    • Tautomerization of bases causing mutations
    • DNA polymerase proofreading
    • UV light damage to DNA
    • DNA repair mechanisms (e.g., repair of UV light damage)
    • Oxidative deamination inducing chemicals
    • Restriction endonucleases
    • Molecular cloning steps
    • Barriers to expressing mammalian genes in prokaryotes
    • Methods to overcome prokaryotic expression barriers

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    Description

    Test your knowledge on the genetic code, translation mechanisms in prokaryotes and eukaryotes, and gene expression regulation. This quiz covers aspects from codons to the roles of various factors involved in translating DNA into proteins.

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