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Lecture 21

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Questions and Answers

What is the function of EcoRI restriction enzyme?

It replicates recombinant DNA within host cells
It makes staggered cuts at specific 6 bp GAATTC palindromic sequence (correct)
It makes blunt cuts at specific 6 bp GAATTC palindromic sequence
It joins sugar phosphate backbones on each strand

What is the role of DNA ligase in recombinant DNA technology?

It makes staggered cuts at specific 6 bp GAATTC palindromic sequence
It replicates recombinant DNA within host cells
It links DNA fragments with either sticky or blunt ends into vector DNA (correct)
It isolates, sequences, and manipulates purified DNA fragment

What is the purpose of T4 DNA ligase in recombinant DNA technology?

It isolates, sequences, and manipulates purified DNA fragment
It makes staggered cuts at specific 6 bp GAATTC palindromic sequence
It covalently joins sugar phosphate backbones on each strand (correct)
It replicates recombinant DNA within host cells

What is the function of vector DNA in plasmid cloning?

It provides the backbone for replicating recombinant DNA within host cells (A) Signup and view all the answers

What is the function of the LacZ alpha gene in plasmid vector screening?

It is used for alpha complementation to screen for successfully ligated plasmids (A) Signup and view all the answers

What is the role of Dicer enzyme in RNA interference (RNAi)?

It cleaves dsRNA into short ds siRNAs (A) Signup and view all the answers

What is the mechanism of action of CRISPR-Cas system in bacteria and archaea?

It constitutes a RNA-mediated defense system against viruses and plasmids (D) Signup and view all the answers

What is the function of the guide RNA in the CRISPR-Cas9 system?

It directs the Cas9 complex to the targeted region of the genome (B) Signup and view all the answers

What is the purpose of homology-directed repair (HDR) in the CRISPR-Cas9 system?

It induces single base changes in the repaired gDNA (D) Signup and view all the answers

What is the function of the synthetic sugar X-gal in plasmid vector screening?

It causes bacteria to turn blue if the vector is successfully transformed (A) Signup and view all the answers

What is the main function of plasmid vectors in DNA cloning?

To permit amplification of DNA fragments (A) Signup and view all the answers

What is the role of RNA interference (RNAi) in gene function regulation?

It can inhibit gene function by using small endogenous miRNAs (C) Signup and view all the answers

What is the function of the ampr gene in plasmid vectors?

It confers resistance to ampicillin (D) Signup and view all the answers

What is the main function of the CRISPR-Cas9 system?

To edit and modify specific genomic sequences (C) Signup and view all the answers