Lecture 21
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Questions and Answers

What is the function of EcoRI restriction enzyme?

  • It replicates recombinant DNA within host cells
  • It makes staggered cuts at specific 6 bp GAATTC palindromic sequence (correct)
  • It makes blunt cuts at specific 6 bp GAATTC palindromic sequence
  • It joins sugar phosphate backbones on each strand

    • What is the role of DNA ligase in recombinant DNA technology?

  • It makes staggered cuts at specific 6 bp GAATTC palindromic sequence
  • It replicates recombinant DNA within host cells
  • It links DNA fragments with either sticky or blunt ends into vector DNA (correct)
  • It isolates, sequences, and manipulates purified DNA fragment

    • What is the purpose of T4 DNA ligase in recombinant DNA technology?

  • It isolates, sequences, and manipulates purified DNA fragment
  • It makes staggered cuts at specific 6 bp GAATTC palindromic sequence
  • It covalently joins sugar phosphate backbones on each strand (correct)
  • It replicates recombinant DNA within host cells

    • What is the function of vector DNA in plasmid cloning?

    <p>It provides the backbone for replicating recombinant DNA within host cells</p> Signup and view all the answers

    What is the function of the LacZ alpha gene in plasmid vector screening?

    <p>It is used for alpha complementation to screen for successfully ligated plasmids</p> Signup and view all the answers

    What is the role of Dicer enzyme in RNA interference (RNAi)?

    <p>It cleaves dsRNA into short ds siRNAs</p> Signup and view all the answers

    What is the mechanism of action of CRISPR-Cas system in bacteria and archaea?

    <p>It constitutes a RNA-mediated defense system against viruses and plasmids</p> Signup and view all the answers

    What is the function of the guide RNA in the CRISPR-Cas9 system?

    <p>It directs the Cas9 complex to the targeted region of the genome</p> Signup and view all the answers

    What is the purpose of homology-directed repair (HDR) in the CRISPR-Cas9 system?

    <p>It induces single base changes in the repaired gDNA</p> Signup and view all the answers

    What is the function of the synthetic sugar X-gal in plasmid vector screening?

    <p>It causes bacteria to turn blue if the vector is successfully transformed</p> Signup and view all the answers

    What is the main function of plasmid vectors in DNA cloning?

    <p>To permit amplification of DNA fragments</p> Signup and view all the answers

    What is the role of RNA interference (RNAi) in gene function regulation?

    <p>It can inhibit gene function by using small endogenous miRNAs</p> Signup and view all the answers

    What is the function of the ampr gene in plasmid vectors?

    <p>It confers resistance to ampicillin</p> Signup and view all the answers

    What is the main function of the CRISPR-Cas9 system?

    <p>To edit and modify specific genomic sequences</p> Signup and view all the answers

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