Lecture 21

It makes staggered cuts at specific 6 bp GAATTC palindromic sequence

It links DNA fragments with either sticky or blunt ends into vector DNA

It covalently joins sugar phosphate backbones on each strand

<p>It provides the backbone for replicating recombinant DNA within host cells</p> Signup and view all the answers

<p>It is used for alpha complementation to screen for successfully ligated plasmids</p> Signup and view all the answers

<p>It cleaves dsRNA into short ds siRNAs</p> Signup and view all the answers

<p>It constitutes a RNA-mediated defense system against viruses and plasmids</p> Signup and view all the answers

<p>It directs the Cas9 complex to the targeted region of the genome</p> Signup and view all the answers

<p>It induces single base changes in the repaired gDNA</p> Signup and view all the answers

<p>It causes bacteria to turn blue if the vector is successfully transformed</p> Signup and view all the answers

<p>To permit amplification of DNA fragments</p> Signup and view all the answers

<p>It can inhibit gene function by using small endogenous miRNAs</p> Signup and view all the answers

<p>It confers resistance to ampicillin</p> Signup and view all the answers

<p>To edit and modify specific genomic sequences</p> Signup and view all the answers