Genes, Protein Synthesis and Transcription

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Questions and Answers

We have already referred to DNA as a code or blueprint for living things. More specifically, it is a code for making _____.

proteins

A sequence of bases = a gene. Each gene on a chromosome “codes” for a protein (aka _____).

polypeptide

If proteins are strings of amino acids, the DNA determines the _____ of the amino acids.

sequence

Protein Synthesis = the _____ of proteins or gene _____.

<p>manufacturing, expression</p> Signup and view all the answers

The DNA is located inside the _____ but proteins are made on the _____.

<p>nucleus, ribosome</p> Signup and view all the answers

Protein Synthesis involves a cousin of DNA, called _____. RNA is similar to DNA, but instead of being double stranded, it is only _____ stranded.

<p>RNA, single</p> Signup and view all the answers

RNA consists of a pentose sugar (_____), a phosphate, and a nitrogenous base. The nitrogenous bases used in RNA are different. The purines are the same (Adenine and Guanine), but the pyrimidines are different (Cytosine and _____).

<p>ribose, uracil</p> Signup and view all the answers

MRNA (_____ RNA), tRNA (_____ RNA) and rRNA (_____ RNA)

<p>messenger, transfer, ribosomal</p> Signup and view all the answers

Transcription starts inside the _____ and involves _____.

<p>nucleus, mRNA</p> Signup and view all the answers

The enzyme _____ Polymerase begins to match up RNA base pairs to the unzipped _____ strand using the RNA pairing rules.

<p>RNA, DNA</p> Signup and view all the answers

These new base pairs form a strand of _____. The mRNA leaves the nucleus through the _____.

<p>mRNA, nuclear pores</p> Signup and view all the answers

The mRNA goes to a _____ and binds to it.

<p>ribosome</p> Signup and view all the answers

Essentially, transcription is all about creating _____.

<p>mRNA</p> Signup and view all the answers

Translation occurs on the _____. The _____ binds to the ribosome.

<p>ribosome, mRNA</p> Signup and view all the answers

The ribosome “reads” the mRNA in sequences of 3 bases at a time. Every 3 bases on the mRNA is called a _____. Each codon codes for an _____ acid.

<p>codon, amino</p> Signup and view all the answers

For example: 3 U's in a row is read as UUU and equals the amino acid _____.

<p>phenylalanine</p> Signup and view all the answers

TRNA molecules match their _____ with the codons on the mRNA molecule and bring with them the correct _____.

<p>anticodons, amino acids</p> Signup and view all the answers

The tRNA molecules drop off their amino acids and leave the ribosome. The growing chain of amino acids eventually forms a _____.

<p>protein</p> Signup and view all the answers

AUG = the amino acid _____. This the universal _____ codon. This tells the ribosome when to _____ protein.

<p>methionine, start, start</p> Signup and view all the answers

UAA, UAG, and UGA are known as the _____ codons. They instruct the ribosome to stop protein synthesis.

<p>stop</p> Signup and view all the answers

Flashcards

What does DNA code for?

Proteins are the product of genes, encoded by DNA sequences.

What is protein synthesis?

Protein synthesis is the process of creating proteins from DNA, involving transcription and translation.

What is RNA?

RNA is a single-stranded nucleic acid similar to DNA, essential for protein synthesis.

What is the role of mRNA?

mRNA carries the genetic code from DNA to ribosomes for protein synthesis.

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What does tRNA do?

tRNA transfers amino acids to the ribosome to build a protein chain.

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What is rRNA's function?

rRNA is a component of ribosomes, facilitating protein assembly.

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What is transcription?

Transcription is the process where DNA's code is copied into mRNA inside the nucleus.

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What is RNA Polymerase?

RNA polymerase matches RNA base pairs to the unzipped DNA strand during transcription.

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What is translation?

Translation is the process where ribosomes read mRNA to assemble amino acids into a protein.

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What is a codon?

A codon is a sequence of three bases on mRNA that codes for a specific amino acid.

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Study Notes

  • DNA serves as a code or blueprint for living things, specifically coding for proteins.

Genes and Protein Synthesis

  • A sequence of bases forms a gene.
  • Each gene on a chromosome codes for a protein, also known as a polypeptide.
  • DNA determines the sequence of amino acids in proteins.
  • Protein synthesis is the manufacturing or expression of proteins or genes.
  • DNA resides in the nucleus, while proteins are made on ribosomes.
  • Protein synthesis involves RNA, a cousin of DNA.
  • RNA is single-stranded, unlike the double-stranded DNA.
  • RNA consists of a ribose sugar, a phosphate, and a nitrogenous base.
  • RNA's nitrogenous bases differ from DNA; while purines are the same (Adenine and Guanine), pyrimidines differ (RNA contains uracil instead of thymine).
  • mRNA (messenger RNA), tRNA (transfer RNA), and rRNA (ribosomal RNA) are the three types of RNA.

Transcription

  • Protein synthesis has two phases: transcription and translation.
  • Transcription occurs in the nucleus and involves mRNA.
  • A region of the DNA unwinds.
  • RNA polymerase matches RNA base pairs to the unzipped DNA strand: A binds with U (in RNA), G binds with C, and C binds with G.
  • New base pairs form a strand of mRNA.
  • mRNA exits the nucleus through nuclear pores.
  • The DNA winds back up.
  • mRNA binds to a ribosome, initiating translation.
  • Transcription essentially creates mRNA.

Translation

  • Translation occurs on the ribosome.
  • mRNA binds to the ribosome.
  • The ribosome reads the mRNA in codons (sequences of 3 bases).
  • Each codon codes for an amino acid, such as UUU coding for phenylalanine.
  • tRNA molecules match their anticodons to mRNA codons, bringing the corresponding amino acids.
  • tRNA molecules drop off their amino acids and leave the ribosome.
  • A protein is formed from the growing chain of amino acids.
  • There are 64 possible codon combinations (using U, A, G, C), but only 20 amino acids, therefore some amino acids are coded for by more than one codon.

Special Condons

  • AUG (methionine) is the universal start codon, signaling the ribosome to begin building the protein; every protein begins with the codon AUG.
  • UAA, UAG, and UGA are stop codons, instructing the ribosome to stop protein synthesis.

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