Bio Labs
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Questions and Answers

What is the correct measurement equivalence of 1 OMU in micrometers?

  • 75 μm/OMU
  • 25 μm/OMU (correct)
  • 0.025 mm/OMU
  • 6 μm/OMU
  • Which of the following components is essential in both PCR reactions for breast cancer gene detection and Alzheimer’s gene detection?

  • primers
  • template
  • buffer
  • all of the above (correct)
  • In the PCR process, which step occurs first in the cycle?

  • annealing
  • extension
  • denaturation (correct)
  • cooling
  • Which statement about the data collection process during the photosynthesis lab is incorrect?

    <p>Both test tubes contained the same solution.</p> Signup and view all the answers

    What is a potential outcome after running PCR for 8 cycles starting with 2 copies of a gene?

    <p>512 copies</p> Signup and view all the answers

    What was the purpose of using a bicarbonate solution in the experiment?

    <p>To serve as a control in the experimental setup.</p> Signup and view all the answers

    Which protein is primarily responsible for synthesizing new DNA strands during PCR?

    <p>DNA polymerase</p> Signup and view all the answers

    What method was employed to measure the photosynthetic rate?

    <p>Dividing the oxygen produced by the time.</p> Signup and view all the answers

    In what way did the order of tasks contribute to the consistency of the experiment?

    <p>Chronologically arranging tasks to ensure precise measurements.</p> Signup and view all the answers

    In humans, how many organelles are known to contain DNA?

    <p>2</p> Signup and view all the answers

    Which aquatic plant species was used in the photosynthesis lab?

    <p>Elodea densa</p> Signup and view all the answers

    Which of these sequences could function as a reverse primer for a given DNA template?

    <p>5' - CCTTCGTTTTCTTGGTGAA - 3'</p> Signup and view all the answers

    What defines the process of annealing in PCR?

    <p>Binding of primers to the template strand</p> Signup and view all the answers

    What was the first step taken by the group to ensure safety before starting the experiment?

    <p>Putting on safety goggles and gloves.</p> Signup and view all the answers

    Which of the following components was included in only one of the test tubes?

    <p>Herbicide</p> Signup and view all the answers

    What was the main purpose of cutting the aquatic plants during the experiment?

    <p>To ensure uniformity in plant height.</p> Signup and view all the answers

    What is the primary purpose of using heat in DNA separation?

    <p>To disrupt hydrogen bonds between complementary base pairs</p> Signup and view all the answers

    Which step is NOT part of the correct procedure when using a micropipettor to transfer liquid?

    <p>Ejecting the tip into a non-designated container</p> Signup and view all the answers

    When moving to a different liquid, what is the correct practice regarding the micropipettor tip?

    <p>The tip should be changed to prevent contamination</p> Signup and view all the answers

    What volume can a P1000 micropipettor dispense when set to 250uL?

    <p>250uL</p> Signup and view all the answers

    What would happen if a micropipettor's plunger is pressed to the second stop when dispensing?

    <p>Excess liquid may be wasted</p> Signup and view all the answers

    Which of the following actions indicates improper use of the micropipettor?

    <p>Holding the micropipettor horizontally</p> Signup and view all the answers

    What is an appropriate volume for a P200 micropipettor set to dispense?

    <p>200uL</p> Signup and view all the answers

    Which of the following is NOT a consequence of using the wrong pipette for a given volume?

    <p>Increased efficiency in liquid transfer</p> Signup and view all the answers

    What would be the photosynthetic rate (mL/min) if the oxygen produced displaces water in the pipette to 0.9mL after 60 minutes?

    <p>1 mL/min</p> Signup and view all the answers

    Which of the following actions is essential to ensure accurate results in a photosynthesis experiment?

    <p>Resetting the pipette to zero before starting</p> Signup and view all the answers

    What is a plausible outcome if DCMU successfully competes for the binding site of the electron transport chain protein?

    <p>A decrease in the rate of photosynthesis</p> Signup and view all the answers

    What factor was not explicitly controlled during the photosynthesis lab according to the memo?

    <p>Distance from the light source</p> Signup and view all the answers

    If the experimental treatment showed a higher displacement than the control, what can be inferred?

    <p>The experimental condition enhanced oxygen production</p> Signup and view all the answers

    What will happen if air is left between the liquid in the tubes and the rubber stopper?

    <p>It will decrease the oxygen production</p> Signup and view all the answers

    During the experimental procedure, which statement is accurate regarding oxygen measurement in relation to CO2?

    <p>The amount of CO2 directly affects the oxygen output</p> Signup and view all the answers

    What is the expected amount of CO2 measured for both experimental and control tubes during the experiment?

    <p>Equal amounts in both tubes</p> Signup and view all the answers

    What is the primary reason for using Chelex in DNA extraction from cheek cells?

    <p>It prevents the degradation of DNA by chelating contaminants.</p> Signup and view all the answers

    After Step #13 of DNA extraction, where can the extracted DNA be found?

    <p>In the supernatant above the pellet.</p> Signup and view all the answers

    When and how are the five PCR components incorporated into the PCR tubes?

    <p>Some components are pre-mixed before being added to the tubes.</p> Signup and view all the answers

    What occurs at the denaturation stage of the PCR program?

    <p>DNA double helix separates into single strands.</p> Signup and view all the answers

    Which primer pair would be suitable to amplify the sequence related to tasting additional bitter compounds?

    <p>5'-TAGAAAAGGAAGGTGGCT-3' and 5'-TTGAAGACGTGGTTGGAA-3'</p> Signup and view all the answers

    What property do gel electrophoresis and PCR have in common?

    <p>They rely on the size of DNA fragments.</p> Signup and view all the answers

    What does the presence of multiple bands in an undigested sample likely indicate?

    <p>The sample was contaminated.</p> Signup and view all the answers

    What best describes the outcome of centrifugation in the context of DNA extraction?

    <p>The components separate into distinct layers.</p> Signup and view all the answers

    What does the presence of two bands visible at approximately 221bp and 44bp indicate about an individual's genotype?

    <p>The individual is heterozygous for the trait.</p> Signup and view all the answers

    Which of the following statements about the PCR amplification process is accurate?

    <p>A thermal cycler is essential for the amplification of target DNA.</p> Signup and view all the answers

    What would happen if the gene responsible for purple kernel color were homozygous for a specific variant?

    <p>The kernel would be yellow due to lack of purple pigment.</p> Signup and view all the answers

    Which set of primers is appropriate for amplifying the sequence of interest from the gene responsible for purple kernel color?

    <p>5'-TAGAAAAGGAAGGTG-3' and 5'-TTGAAGACGTGGTTG-3'</p> Signup and view all the answers

    What component is crucial for PCR amplification that if missing would result in no visible bands?

    <p>Template DNA</p> Signup and view all the answers

    What effect does introducing the restriction enzyme EcoRV have on DNA?

    <p>It cleaves the DNA at specific sites.</p> Signup and view all the answers

    Which method would be most effective in removing impurities like Mg2+ during DNA extraction?

    <p>Using Chelex resin.</p> Signup and view all the answers

    In the experiment to determine genotype, what is the expected result if the reaction includes a primer dimer?

    <p>The bands will show additional smaller fragments.</p> Signup and view all the answers

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