Podcast
Questions and Answers
What is the pKa of HEPES?
What is the pKa of HEPES?
Higher concentrations of a buffer will result in lower buffering capacity.
Higher concentrations of a buffer will result in lower buffering capacity.
False
What is the desired pH value for the HEPES buffer preparation?
What is the desired pH value for the HEPES buffer preparation?
7.3
To adjust the pH of the HEPES buffer, one must use _____ until the desired pH is attained.
To adjust the pH of the HEPES buffer, one must use _____ until the desired pH is attained.
Signup and view all the answers
Match the following components of HEPES preparation with their corresponding amounts:
Match the following components of HEPES preparation with their corresponding amounts:
Signup and view all the answers
How much HEPES sodium salt (A-) is needed to make the buffer?
How much HEPES sodium salt (A-) is needed to make the buffer?
Signup and view all the answers
The total concentration of HA and A- in the buffer must equal 50mM.
The total concentration of HA and A- in the buffer must equal 50mM.
Signup and view all the answers
What is the total volume of the HEPES buffer solution after preparation?
What is the total volume of the HEPES buffer solution after preparation?
Signup and view all the answers
What is the pH of electrically neutral water at 25ºC?
What is the pH of electrically neutral water at 25ºC?
Signup and view all the answers
Buffers completely dissociate in solution to control pH effectively.
Buffers completely dissociate in solution to control pH effectively.
Signup and view all the answers
What term is used to describe molecules that help minimize pH changes in a solution?
What term is used to describe molecules that help minimize pH changes in a solution?
Signup and view all the answers
The equation for calculating pH is pH = -log [____].
The equation for calculating pH is pH = -log [____].
Signup and view all the answers
Match the following pairs of terms with their descriptions:
Match the following pairs of terms with their descriptions:
Signup and view all the answers
Which of the following statements is true regarding the Henderson-Hasselbalch equation?
Which of the following statements is true regarding the Henderson-Hasselbalch equation?
Signup and view all the answers
If the concentration of the conjugate base is greater than that of the weak acid, the pH will be less than the pKa.
If the concentration of the conjugate base is greater than that of the weak acid, the pH will be less than the pKa.
Signup and view all the answers
What would happen to the pH of a buffer solution if a strong acid was added?
What would happen to the pH of a buffer solution if a strong acid was added?
Signup and view all the answers
What happens when the energy of an incoming photon exactly matches the energy difference between two energy levels?
What happens when the energy of an incoming photon exactly matches the energy difference between two energy levels?
Signup and view all the answers
The return of the excited electron to a lower energy state is associated with a loss of heat.
The return of the excited electron to a lower energy state is associated with a loss of heat.
Signup and view all the answers
How long does it take for the absorption process to occur?
How long does it take for the absorption process to occur?
Signup and view all the answers
The instrument commonly used to measure the absorption of light by a substance is called a __________.
The instrument commonly used to measure the absorption of light by a substance is called a __________.
Signup and view all the answers
Match the light sources with their primary emission ranges:
Match the light sources with their primary emission ranges:
Signup and view all the answers
Which of the following is NOT a consideration when measuring absorption?
Which of the following is NOT a consideration when measuring absorption?
Signup and view all the answers
Molecules remain excited for a long period of time after absorption occurs.
Molecules remain excited for a long period of time after absorption occurs.
Signup and view all the answers
What must be done to quantify the process of absorption into something useful?
What must be done to quantify the process of absorption into something useful?
Signup and view all the answers
What is the primary function of a buffer according to the Henderson-Hasselbalch equation?
What is the primary function of a buffer according to the Henderson-Hasselbalch equation?
Signup and view all the answers
A buffer can effectively resist changes in pH when the pH is more than 1 unit away from the pKa.
A buffer can effectively resist changes in pH when the pH is more than 1 unit away from the pKa.
Signup and view all the answers
What is the typical rule of thumb for selecting a buffer in relation to its pKa?
What is the typical rule of thumb for selecting a buffer in relation to its pKa?
Signup and view all the answers
Buffer capacity is defined as the number of moles of [H+] required to lower 1L of the buffer ___ pH unit.
Buffer capacity is defined as the number of moles of [H+] required to lower 1L of the buffer ___ pH unit.
Signup and view all the answers
Match the following terms with their definitions:
Match the following terms with their definitions:
Signup and view all the answers
In a scenario where a buffer with a pKa of 8.0 has 10 molecules, how many molecules of HA will remain after adding 4 molecules of NaOH?
In a scenario where a buffer with a pKa of 8.0 has 10 molecules, how many molecules of HA will remain after adding 4 molecules of NaOH?
Signup and view all the answers
Increasing the number of buffer molecules increases the buffer's capacity.
Increasing the number of buffer molecules increases the buffer's capacity.
Signup and view all the answers
When equal amounts of HA and A are present in a buffer, what can be concluded about the pH relative to the pKa?
When equal amounts of HA and A are present in a buffer, what can be concluded about the pH relative to the pKa?
Signup and view all the answers
What is the correct mass of HEPES required to prepare a 50 mM solution in 1L of water?
What is the correct mass of HEPES required to prepare a 50 mM solution in 1L of water?
Signup and view all the answers
The absorption of electromagnetic radiation can be described as a quantum phenomenon.
The absorption of electromagnetic radiation can be described as a quantum phenomenon.
Signup and view all the answers
What is the relationship between the energy of photons and their wavelength?
What is the relationship between the energy of photons and their wavelength?
Signup and view all the answers
The lowest energy state of a molecule is known as the ________ state.
The lowest energy state of a molecule is known as the ________ state.
Signup and view all the answers
Which of the following accurately describes the role of a photon in absorption?
Which of the following accurately describes the role of a photon in absorption?
Signup and view all the answers
Match the following energy states with their corresponding descriptions:
Match the following energy states with their corresponding descriptions:
Signup and view all the answers
The formula used to calculate the volume of solution needed is ________.
The formula used to calculate the volume of solution needed is ________.
Signup and view all the answers
The Jablonski diagram shows different vibrational states within energy levels.
The Jablonski diagram shows different vibrational states within energy levels.
Signup and view all the answers
Study Notes
No Specific Topic Provided
- No information was given to generate study notes. Please provide text or questions.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Related Documents
Description
This quiz is designed for general topics where specific information was not provided. Test your knowledge and explore a variety of questions that cover a broad spectrum of subjects without specific focus.
