Podcast
Questions and Answers
Which of the following best describes the process of genetic engineering?
Which of the following best describes the process of genetic engineering?
- Studying the different segments of DNA without any modifications.
- Observing natural variations in organisms over time.
- Breeding organisms with desirable traits together.
- Altering an organism's characteristics by directly manipulating its DNA. (correct)
Genetic engineering is the same as genetic modification.
Genetic engineering is the same as genetic modification.
True (A)
What is the primary function of a vector in genetic engineering?
What is the primary function of a vector in genetic engineering?
A vector carries the desired gene into the host cell's nucleus.
Genetic engineering provides techniques to ______ DNA, either randomly or at specific sites.
Genetic engineering provides techniques to ______ DNA, either randomly or at specific sites.
Match the following terms with their corresponding descriptions:
Match the following terms with their corresponding descriptions:
What is the significant implication of shuffling genetic information between unrelated species?
What is the significant implication of shuffling genetic information between unrelated species?
Genetic engineering is only used in the agricultural industry.
Genetic engineering is only used in the agricultural industry.
What is one example of a genetically modified organism (GMO) and its specific benefit?
What is one example of a genetically modified organism (GMO) and its specific benefit?
What is the primary purpose of introducing the genetically modified plasmid into bacteria or yeast cells?
What is the primary purpose of introducing the genetically modified plasmid into bacteria or yeast cells?
The process of genetic modification involves inserting the gene for human insulin into a ______ which is then introduced into bacterial or yeast cells.
The process of genetic modification involves inserting the gene for human insulin into a ______ which is then introduced into bacterial or yeast cells.
Genetic engineering in its modern form has been around for over 50 years.
Genetic engineering in its modern form has been around for over 50 years.
What is the key benefit of using large fermentation vessels to grow the genetically modified bacteria or yeast?
What is the key benefit of using large fermentation vessels to grow the genetically modified bacteria or yeast?
Match the following techniques with their descriptions:
Match the following techniques with their descriptions:
What is one social consequence of genetic engineering that makes the risk/benefit assessment complex?
What is one social consequence of genetic engineering that makes the risk/benefit assessment complex?
The deliberate encouragement of specific crosses in animal breeding is a recent practice, developed in the past few decades.
The deliberate encouragement of specific crosses in animal breeding is a recent practice, developed in the past few decades.
Which of the following techniques is NOT regarded as genetic modification in EU regulations?
Which of the following techniques is NOT regarded as genetic modification in EU regulations?
What is a hormone?
What is a hormone?
Where in the body is insulin produced?
Where in the body is insulin produced?
What is the function of insulin?
What is the function of insulin?
Why are bacteria suitable for use in producing human insulin through genetic engineering?
Why are bacteria suitable for use in producing human insulin through genetic engineering?
The biomolecules used to extract a gene from a chromosome are _______ and _______.
The biomolecules used to extract a gene from a chromosome are _______ and _______.
The same restriction enzyme must be used to extract the gene from a chromosome and open the loop of DNA in a bacterium for successful genetic engineering because it ensures that the gene is inserted into the bacterial DNA at the right location and orientation.
The same restriction enzyme must be used to extract the gene from a chromosome and open the loop of DNA in a bacterium for successful genetic engineering because it ensures that the gene is inserted into the bacterial DNA at the right location and orientation.
What substances should be added to a bioreactor to enable bacteria to grow?
What substances should be added to a bioreactor to enable bacteria to grow?
Give one advantage of using genetically engineered insulin compared to that extracted from pigs, sheep, or cattle.
Give one advantage of using genetically engineered insulin compared to that extracted from pigs, sheep, or cattle.
Match the sheep in the cloning figure with their role in the process:
Match the sheep in the cloning figure with their role in the process:
Post-zygotic barriers prevent further reproduction from occurring after fertilization and a ______ is formed.
Post-zygotic barriers prevent further reproduction from occurring after fertilization and a ______ is formed.
Reduced hybrid viability occurs when offspring are sterile but able to survive.
Reduced hybrid viability occurs when offspring are sterile but able to survive.
Which of the following is NOT a common example of post-zygotic barriers?
Which of the following is NOT a common example of post-zygotic barriers?
What is the main difference between allopatric and sympatric speciation? Explain.
What is the main difference between allopatric and sympatric speciation? Explain.
Match the following examples of post-zygotic barriers with their descriptions:
Match the following examples of post-zygotic barriers with their descriptions:
What is the result of polyploidy in sympatric speciation?
What is the result of polyploidy in sympatric speciation?
Allopatric speciation can only occur when there is a physical barrier separating populations.
Allopatric speciation can only occur when there is a physical barrier separating populations.
What is a common example of sympatric speciation? Describe the process involved.
What is a common example of sympatric speciation? Describe the process involved.
What is the primary role of hormones in the body?
What is the primary role of hormones in the body?
Insulin is produced in the kidneys.
Insulin is produced in the kidneys.
What is the function of insulin in the body?
What is the function of insulin in the body?
The ______ enzyme is used to extract a gene from a chromosome.
The ______ enzyme is used to extract a gene from a chromosome.
Why are bacteria considered suitable for insulin production?
Why are bacteria considered suitable for insulin production?
Match the following sheep with their contribution in the cloning process:
Match the following sheep with their contribution in the cloning process:
What key process led to the emergence of aerobic eukaryotic cells?
What key process led to the emergence of aerobic eukaryotic cells?
A clone is created when two different organisms reproduce.
A clone is created when two different organisms reproduce.
The Cambrian explosion occurred over a period of 100 million years.
The Cambrian explosion occurred over a period of 100 million years.
Define evolution in a single sentence.
Define evolution in a single sentence.
What is the significance of transferring genetic materials from parents to offspring?
What is the significance of transferring genetic materials from parents to offspring?
The first unicellular organisms were found in fossilized __________.
The first unicellular organisms were found in fossilized __________.
Match the following events in Earth's history with their significance:
Match the following events in Earth's history with their significance:
What describes the cumulative changes in allele frequency of a population over generations?
What describes the cumulative changes in allele frequency of a population over generations?
Evolution occurs within a single generation.
Evolution occurs within a single generation.
What impact did the two-year drought have on the beetle population's size?
What impact did the two-year drought have on the beetle population's size?
Flashcards
Genetic Engineering
Genetic Engineering
The direct manipulation of DNA to alter an organism's characteristics.
Recombinant DNA (rDNA)
Recombinant DNA (rDNA)
DNA that has been artificially created by combining DNA from different sources.
Isolation of DNA
Isolation of DNA
The process of extracting DNA from an organism for study.
Insertion of DNA
Insertion of DNA
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Genetic Vectors
Genetic Vectors
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Expression of Traits
Expression of Traits
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Species Barrier
Species Barrier
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DNA Splicing
DNA Splicing
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Insulin
Insulin
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Pancreas
Pancreas
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Bacteria in insulin production
Bacteria in insulin production
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Restriction enzyme
Restriction enzyme
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Bioreactor
Bioreactor
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Clone
Clone
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Ethical concerns of genetic engineering
Ethical concerns of genetic engineering
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Genetically Modified Plasmid
Genetically Modified Plasmid
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Insulin Production
Insulin Production
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Fermentation Vessels
Fermentation Vessels
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Insulin Purification
Insulin Purification
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Transgenesis
Transgenesis
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Reproductive Techniques
Reproductive Techniques
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Bacterial Conjugation
Bacterial Conjugation
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Environmental Challenges Selection
Environmental Challenges Selection
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Oxygen Revolution
Oxygen Revolution
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Endosymbiosis
Endosymbiosis
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Cambrian Explosion
Cambrian Explosion
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Heritable Traits
Heritable Traits
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Evolution
Evolution
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Allele Frequency
Allele Frequency
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Natural Selection
Natural Selection
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Population Genetics
Population Genetics
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Post-zygotic barriers
Post-zygotic barriers
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Reduced Hybrid Viability
Reduced Hybrid Viability
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Reduced Hybrid Fertility
Reduced Hybrid Fertility
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Hybrid Breakdown
Hybrid Breakdown
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Allopatric Speciation
Allopatric Speciation
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Sympatric Speciation
Sympatric Speciation
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Polyploidy
Polyploidy
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Examples of Speciation
Examples of Speciation
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Hormone
Hormone
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Cloning
Cloning
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Fused Cell
Fused Cell
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Nucleus Removal
Nucleus Removal
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Genetic Transfer
Genetic Transfer
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Study Notes
General Biology 2
- Course title: General Biology 2
- Quarter: 3
- Module: 1-4
Module 1: Genetic Engineering Process
- Genetic engineering manipulates an organism's DNA to change characteristics.
- Recombinant DNA technology alters the genetic makeup.
- The process isolates DNA from one organism, inserts it into another, ensuring the inserted genes are expressed.
- Techniques cut DNA—randomly or at specific sites.
- Vectors (plasmids or viruses) carry the gene into the host cell.
- Shotgun technique shoots genes into cells for insertion.
- The recipient cell needs a "promoter" (signal) for the gene to function.
- Viral promoters are frequently used to regulate gene expression in plants.
- Viral DNA integrates into host cell DNA, multiplying and infecting new cells.
- Genetic engineering methods ensure gene insertion for new species traits.
- This process frequently uses a control element for function and insertion for a given species.
- The process is not precise or predictable.
Module 1: Example of Genetic Engineering (Insulin)
- Insulin regulates blood sugar.
- Type 1 diabetes involves insulin production problems needing treatment.
- Genetic engineering produces human-like insulin from yeast or bacteria.
- A plasmid (circular DNA) is extracted from bacteria/yeast.
- Using restriction enzymes, a DNA section is cut.
- The human insulin gene is inserted into the plasmid.
- The plasmid is put into a new bacteria/yeast cell.
- The bacteria multiply rapidly, producing insulin.
- The insulin is purified and packaged for distribution.
- "Humulin" insulin was the first genetically engineered insulin product for human use in 1982.
Module 2: Mechanism of Evolution
- Evolution: Heritable trait changes over time in species.
- Modern organisms descend from a common ancestor.
- Geological time scale categorizes Earth's history.
- Epochs, periods, eras, and eons constitute the scale.
- The earliest life existed about 3.5 billion years ago.
- Fossils document life's evolution.
- Significant historical events like mass extinctions define scale units.
- The fossil record shows biodiversity changes over time.
- Selective forces (natural selection, etc.) drive evolutionary changes.
Module 2: Mechanisms of Evolution
- Mutation: nucleotide sequence changes in DNA, creating new alleles.
- Genetic drift: Random changes in allele frequency, mainly in small populations (founder and bottleneck effects).
- Natural selection: organisms adapt to environmental pressures. Organisms with beneficial traits survive more effectively.
- Artificial selection: Human-driven breeding to create desired traits in plants and animals.
Module 3: Speciation and Development of Evolutionary Thought
- Species: Population of organisms that have the potential to interbreed.
- Speciation: Formation of new species.
- Reproductive isolation leads to speciation.
- Prezygotic barriers: prevent fertilization (habitat, temporal, behavioral, mechanical, gametic isolation).
- Postzygotic barriers: prevent development of fertile offspring (reduced viability, reduced fertility, hybrid breakdown).
- Allopatric speciation: Geographic isolation leads to separate species evolution.
- Sympatric speciation: Speciation occurring within the same geographic area.
Module 4: Evidences of Evolution
- Biogeography: Geographical distribution of organisms and the conditions affecting their presence.
- Fossils: Preserved remains of past organisms, showing evolutionary sequences.
- Homology: Similar characteristics resulting from common ancestry (anatomical structures, DNA, proteins).
- Vestigial structures: Remnants of previously functional structures in organisms, suggesting evolutionary changes.
- Embryology: Developmental similarities among various organisms, suggesting shared ancestry.
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