General Biology 2: Genetic Engineering Process

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Questions and Answers

Which of the following best describes the process of genetic engineering?

  • Studying the different segments of DNA without any modifications.
  • Observing natural variations in organisms over time.
  • Breeding organisms with desirable traits together.
  • Altering an organism's characteristics by directly manipulating its DNA. (correct)

Genetic engineering is the same as genetic modification.

True (A)

What is the primary function of a vector in genetic engineering?

A vector carries the desired gene into the host cell's nucleus.

Genetic engineering provides techniques to ______ DNA, either randomly or at specific sites.

<p>cut</p> Signup and view all the answers

Match the following terms with their corresponding descriptions:

<p>Recombinant DNA (rDNA) = DNA that combines genetic material from different sources. Vector = A carrier that delivers a gene into a host cell. Genetic modification = Alteration of an organism's genetic makeup.</p> Signup and view all the answers

What is the significant implication of shuffling genetic information between unrelated species?

<p>It can create organisms with desirable traits. (A), It creates opportunities for studying the function of different genes. (B), It poses potential risks to biodiversity. (C)</p> Signup and view all the answers

Genetic engineering is only used in the agricultural industry.

<p>False (B)</p> Signup and view all the answers

What is one example of a genetically modified organism (GMO) and its specific benefit?

<p>Examples: Golden Rice (increased Vitamin A content), BT Corn (resistance to insect pests), Insulin-producing bacteria (treatment of diabetes). Benefits can be anything like increased nutritional content, pest resistance, disease resistance, or production of therapeutic substances.</p> Signup and view all the answers

What is the primary purpose of introducing the genetically modified plasmid into bacteria or yeast cells?

<p>To allow the cells to produce insulin (B)</p> Signup and view all the answers

The process of genetic modification involves inserting the gene for human insulin into a ______ which is then introduced into bacterial or yeast cells.

<p>plasmid</p> Signup and view all the answers

Genetic engineering in its modern form has been around for over 50 years.

<p>False (B)</p> Signup and view all the answers

What is the key benefit of using large fermentation vessels to grow the genetically modified bacteria or yeast?

<p>This allows for the production of large quantities of insulin.</p> Signup and view all the answers

Match the following techniques with their descriptions:

<p>Bacterial transduction = Using phages (bacterial viruses) to transfer genetic material Traditional breeding = Deliberate crossing of varieties of species Selection of newborn = Choosing individuals with desirable traits based on environmental challenges Manipulation of bacterial conjugation = Interfering with the natural process of bacterial gene transfer</p> Signup and view all the answers

What is one social consequence of genetic engineering that makes the risk/benefit assessment complex?

<p>There are a variety of ethical and societal concerns surrounding genetic modification.</p> Signup and view all the answers

The deliberate encouragement of specific crosses in animal breeding is a recent practice, developed in the past few decades.

<p>False (B)</p> Signup and view all the answers

Which of the following techniques is NOT regarded as genetic modification in EU regulations?

<p>Manipulation of bacterial conjugation (B)</p> Signup and view all the answers

What is a hormone?

<p>A hormone is a chemical messenger produced by the body that regulates specific physiological processes.</p> Signup and view all the answers

Where in the body is insulin produced?

<p>Insulin is produced in the pancreas, specifically by the beta cells of the islets of Langerhans.</p> Signup and view all the answers

What is the function of insulin?

<p>Insulin's main function is to regulate blood glucose levels by facilitating the uptake of glucose from the bloodstream into cells for energy production.</p> Signup and view all the answers

Why are bacteria suitable for use in producing human insulin through genetic engineering?

<p>Bacteria are simple organisms with a relatively small genome, making genetic manipulation easier. (A), Bacteria reproduce rapidly, allowing for efficient production of the desired protein. (B), Bacteria can be grown in large quantities at low cost. (C), All of the above. (D)</p> Signup and view all the answers

The biomolecules used to extract a gene from a chromosome are _______ and _______.

<p>enzymes, restriction</p> Signup and view all the answers

The same restriction enzyme must be used to extract the gene from a chromosome and open the loop of DNA in a bacterium for successful genetic engineering because it ensures that the gene is inserted into the bacterial DNA at the right location and orientation.

<p>True (A)</p> Signup and view all the answers

What substances should be added to a bioreactor to enable bacteria to grow?

<p>Nutrients, such as sugars and amino acids. (A), Oxygen. (B), Temperature control mechanisms. (C), All of the above. (D)</p> Signup and view all the answers

Give one advantage of using genetically engineered insulin compared to that extracted from pigs, sheep, or cattle.

<p>Genetically engineered insulin is less likely to cause allergic reactions in patients.</p> Signup and view all the answers

Match the sheep in the cloning figure with their role in the process:

<p>Dolly = Nuclear donor sheep A = Egg cell donor sheep B = Surrogate mother sheep</p> Signup and view all the answers

Post-zygotic barriers prevent further reproduction from occurring after fertilization and a ______ is formed.

<p>zygote</p> Signup and view all the answers

Reduced hybrid viability occurs when offspring are sterile but able to survive.

<p>False (B)</p> Signup and view all the answers

Which of the following is NOT a common example of post-zygotic barriers?

<p>Habitat isolation (B)</p> Signup and view all the answers

What is the main difference between allopatric and sympatric speciation? Explain.

<p>Allopatric speciation occurs when populations are geographically isolated, while sympatric speciation occurs in populations that live in the same geographic area.</p> Signup and view all the answers

Match the following examples of post-zygotic barriers with their descriptions:

<p>Reduced hybrid viability = The offspring fail to develop past the early embryonic stages or die. Reduced hybrid fertility = The offspring is viable but sterile. Hybrid breakdown = Offspring of the first generation are fertile, but subsequent generations become sterile.</p> Signup and view all the answers

What is the result of polyploidy in sympatric speciation?

<p>Formation of new species (B)</p> Signup and view all the answers

Allopatric speciation can only occur when there is a physical barrier separating populations.

<p>True (A)</p> Signup and view all the answers

What is a common example of sympatric speciation? Describe the process involved.

<p>A common example of sympatric speciation is the formation of new plant species through polyploidy. This happens when an individual has more than one set of chromosomes, which can lead to reproductive isolation from the original species and the formation of a new species.</p> Signup and view all the answers

What is the primary role of hormones in the body?

<p>Acting as messengers to control body activities (A)</p> Signup and view all the answers

Insulin is produced in the kidneys.

<p>False (B)</p> Signup and view all the answers

What is the function of insulin in the body?

<p>To reduce the concentration of glucose in the blood</p> Signup and view all the answers

The ______ enzyme is used to extract a gene from a chromosome.

<p>restriction</p> Signup and view all the answers

Why are bacteria considered suitable for insulin production?

<p>They have a faster reproduction rate than other organisms. (A)</p> Signup and view all the answers

Match the following sheep with their contribution in the cloning process:

<p>Sheep A = Source of the nucleus Sheep B = Provider of the egg cell</p> Signup and view all the answers

What key process led to the emergence of aerobic eukaryotic cells?

<p>Endosymbiosis (B)</p> Signup and view all the answers

A clone is created when two different organisms reproduce.

<p>False (B)</p> Signup and view all the answers

The Cambrian explosion occurred over a period of 100 million years.

<p>False (B)</p> Signup and view all the answers

Define evolution in a single sentence.

<p>Evolution is descent with modification or changes of traits in species over time.</p> Signup and view all the answers

What is the significance of transferring genetic materials from parents to offspring?

<p>It leads to the inheritance of traits from one generation to the next.</p> Signup and view all the answers

The first unicellular organisms were found in fossilized __________.

<p>stromatolites</p> Signup and view all the answers

Match the following events in Earth's history with their significance:

<p>Oxygen Revolution = Increase of O2 in the atmosphere Cambrian Explosion = Sudden appearance of various animal groups Endosymbiosis = Eukaryotic cells evolving from prokaryotes Mass Extinctions = Significant loss of biodiversity</p> Signup and view all the answers

What describes the cumulative changes in allele frequency of a population over generations?

<p>Evolution (B)</p> Signup and view all the answers

Evolution occurs within a single generation.

<p>False (B)</p> Signup and view all the answers

What impact did the two-year drought have on the beetle population's size?

<p>The size of the beetles decreased compared to previous generations.</p> Signup and view all the answers

Flashcards

Genetic Engineering

The direct manipulation of DNA to alter an organism's characteristics.

Recombinant DNA (rDNA)

DNA that has been artificially created by combining DNA from different sources.

Isolation of DNA

The process of extracting DNA from an organism for study.

Insertion of DNA

The technique of placing DNA into another organism’s genome.

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Genetic Vectors

Tools, often plasmids or viruses, used to deliver foreign DNA into a host cell.

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Expression of Traits

When the inserted genes are activated in the modified organism.

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Species Barrier

The natural limit to gene transfer between different species.

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DNA Splicing

The process of cutting DNA and inserting sequences from other DNA.

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Insulin

A hormone produced by the pancreas that regulates blood sugar levels.

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Pancreas

An organ that produces insulin and digestive enzymes in the body.

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Bacteria in insulin production

Bacteria can be genetically modified to produce human insulin effectively.

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Restriction enzyme

An enzyme that cuts DNA at specific sequences to extract genes.

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Bioreactor

A vessel that provides a controlled environment for biological reactions, such as bacterial growth.

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Clone

An organism that is genetically identical to another organism.

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Ethical concerns of genetic engineering

Significant issues related to morality and consequences of modifying animals genetically.

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Genetically Modified Plasmid

A plasmid that has been altered to contain desired genes, such as the human insulin gene.

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Insulin Production

Bacteria or yeast cells that rapidly divide and produce insulin, facilitated by genetically modified plasmids.

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Fermentation Vessels

Large containers used to grow genetically modified bacteria or yeast, providing necessary nutrients for rapid division.

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Insulin Purification

The process of filtering the fermentation mixture to extract and clean insulin for distribution.

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Transgenesis

A form of genetic engineering where genes are transferred from one organism to another to obtain desired traits.

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Reproductive Techniques

Methods of artificial interference in breeding processes to shape traits in microorganisms, plants, and animals.

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Bacterial Conjugation

The process by which bacteria transfer genetic material through direct cell-to-cell contact.

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Environmental Challenges Selection

The process of selecting traits in newborns based on environmental pressures, often seen in historical contexts.

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Oxygen Revolution

A significant increase of O2 in the atmosphere that impacted anaerobic organisms.

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Endosymbiosis

The process where prokaryotic cells engulfed mitochondria, leading to the evolution of eukaryotes.

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Cambrian Explosion

A rapid diversification of life that occurred around 535-525 million years ago.

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Heritable Traits

Genetic characteristics that can be passed from generation to generation.

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Evolution

Descent with modification; changes in traits or allele frequencies in populations over time.

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Allele Frequency

The ratio of a specific allele to the total number of alleles in a gene pool.

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Natural Selection

The process whereby organisms better adapted to their environment tend to survive and produce more offspring.

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Population Genetics

The study of how gene frequencies in a population change over time.

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Post-zygotic barriers

Factors that prevent offspring from developing or reproducing after fertilization.

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Reduced Hybrid Viability

Offspring may fail to develop fully or survive to maturity.

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Reduced Hybrid Fertility

Hybrids may be viable but sterile, unable to reproduce.

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Hybrid Breakdown

Fertile first-generation hybrids may produce sterile offspring in later generations.

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Allopatric Speciation

Speciation occurring when populations are geographically isolated.

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Sympatric Speciation

Speciation occurring in overlapping populations within the same area.

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Polyploidy

Condition in which an organism has extra sets of chromosomes.

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Examples of Speciation

Various ways species can form, either through isolation or shared environments.

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Hormone

A chemical substance secreted by endocrine glands that acts as a messenger to control body activities.

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Cloning

The process of creating a genetically identical organism from a cell.

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Fused Cell

A cell formed by combining the nucleus of one cell with the egg cell of another.

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Nucleus Removal

The act of taking the nucleus out of an egg cell before cloning.

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Genetic Transfer

The process of passing genetic material from parents to offspring during reproduction.

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Study Notes

General Biology 2

  • Course title: General Biology 2
  • Quarter: 3
  • Module: 1-4

Module 1: Genetic Engineering Process

  • Genetic engineering manipulates an organism's DNA to change characteristics.
  • Recombinant DNA technology alters the genetic makeup.
  • The process isolates DNA from one organism, inserts it into another, ensuring the inserted genes are expressed.
  • Techniques cut DNA—randomly or at specific sites.
  • Vectors (plasmids or viruses) carry the gene into the host cell.
  • Shotgun technique shoots genes into cells for insertion.
  • The recipient cell needs a "promoter" (signal) for the gene to function.
  • Viral promoters are frequently used to regulate gene expression in plants.
  • Viral DNA integrates into host cell DNA, multiplying and infecting new cells.
  • Genetic engineering methods ensure gene insertion for new species traits.
  • This process frequently uses a control element for function and insertion for a given species.
  • The process is not precise or predictable.

Module 1: Example of Genetic Engineering (Insulin)

  • Insulin regulates blood sugar.
  • Type 1 diabetes involves insulin production problems needing treatment.
  • Genetic engineering produces human-like insulin from yeast or bacteria.
  • A plasmid (circular DNA) is extracted from bacteria/yeast.
  • Using restriction enzymes, a DNA section is cut.
  • The human insulin gene is inserted into the plasmid.
  • The plasmid is put into a new bacteria/yeast cell.
  • The bacteria multiply rapidly, producing insulin.
  • The insulin is purified and packaged for distribution.
  • "Humulin" insulin was the first genetically engineered insulin product for human use in 1982.

Module 2: Mechanism of Evolution

  • Evolution: Heritable trait changes over time in species.
  • Modern organisms descend from a common ancestor.
  • Geological time scale categorizes Earth's history.
  • Epochs, periods, eras, and eons constitute the scale.
  • The earliest life existed about 3.5 billion years ago.
  • Fossils document life's evolution.
  • Significant historical events like mass extinctions define scale units.
  • The fossil record shows biodiversity changes over time.
  • Selective forces (natural selection, etc.) drive evolutionary changes.

Module 2: Mechanisms of Evolution

  • Mutation: nucleotide sequence changes in DNA, creating new alleles.
  • Genetic drift: Random changes in allele frequency, mainly in small populations (founder and bottleneck effects).
  • Natural selection: organisms adapt to environmental pressures. Organisms with beneficial traits survive more effectively.
  • Artificial selection: Human-driven breeding to create desired traits in plants and animals.

Module 3: Speciation and Development of Evolutionary Thought

  • Species: Population of organisms that have the potential to interbreed.
  • Speciation: Formation of new species.
  • Reproductive isolation leads to speciation.
  • Prezygotic barriers: prevent fertilization (habitat, temporal, behavioral, mechanical, gametic isolation).
  • Postzygotic barriers: prevent development of fertile offspring (reduced viability, reduced fertility, hybrid breakdown).
  • Allopatric speciation: Geographic isolation leads to separate species evolution.
  • Sympatric speciation: Speciation occurring within the same geographic area.

Module 4: Evidences of Evolution

  • Biogeography: Geographical distribution of organisms and the conditions affecting their presence.
  • Fossils: Preserved remains of past organisms, showing evolutionary sequences.
  • Homology: Similar characteristics resulting from common ancestry (anatomical structures, DNA, proteins).
  • Vestigial structures: Remnants of previously functional structures in organisms, suggesting evolutionary changes.
  • Embryology: Developmental similarities among various organisms, suggesting shared ancestry.

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