Gene Therapy: Principles and Applications

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What is the primary goal of gene therapy?

To make a beneficial protein to compensate for abnormal genes

To introduce a new gene into the body to help fight a disease

To use drugs or surgery to treat a disorder

To replace a mutated gene that causes disease with a healthy copy of the gene (correct)

What is the current status of gene therapy?

It has been proven to be completely safe and effective

It has been abandoned due to its risks

It is still under study to ensure its safety and effectiveness (correct)

It is widely used to treat many diseases

What is the role of viruses in gene therapy?

They are used to make beneficial proteins

They are used to deliver new genes into cells (correct)

They are used to replace mutated genes

They are used to inactivate faulty genes

What type of diseases is gene therapy currently being tested for?

Diseases that have no other cures Signup and view all the answers

What is the purpose of introducing a new gene into the body?

To make a beneficial protein to fight a disease Signup and view all the answers

What is the function of the normal copy of the gene introduced through gene therapy?

To restore the function of a necessary protein Signup and view all the answers

What is the primary role of collagenase in treating skin ulcers and burns?

Breaking and removing dead skin Signup and view all the answers

What is the primary source of lytic enzymes?

Bacteriophage Signup and view all the answers

What is the primary function of chitinase?

Hydrolyzing chitin Signup and view all the answers

What is the primary factor influencing the immune response to non-viral DNA complexes?

Dose of the complex Signup and view all the answers

What is the primary target of most antimicrobials?

Chitin Signup and view all the answers

What is the effect of infection and inflammation on cytochrome P450 enzymes?

Reduced expression and function Signup and view all the answers

What is the primary application of lysozyme?

Treating viral infections Signup and view all the answers

What is the current status of gene therapy as a treatment option?

Under study to determine its safety and efficacy Signup and view all the answers

What is the primary concern that has led to efforts to develop new treatments?

Cancer Signup and view all the answers

What is the role of the RES in the immune response to non-viral DNA complexes?

Recognition of large particulates Signup and view all the answers

What is a potential consequence of using high doses of cationic liposomes in gene therapy?

Acute inflammation and tissue damage Signup and view all the answers

What is a potential health risk associated with gene therapy?

Cancer Signup and view all the answers

What is the primary reason Naked DNA is ineffective in gene transfer?

Susceptibility to nuclease degradation in the systemic circulation Signup and view all the answers

What is the primary mechanism of liposomal gene delivery?

Formation of organized structures with DNA through positive charge interaction Signup and view all the answers

What is the function of the spacer in a cationic lipid?

To vary the length of the lipid structure Signup and view all the answers

What is the primary advantage of microinjection in gene transfer?

Direct delivery of genetic material to the cytoplasm or nucleus Signup and view all the answers

What is the primary factor affecting the interaction of liposomes with DNA?

pH, charge, and lipid structure Signup and view all the answers

What is the primary limitation of early physical methods for gene transfer?

Rudimentary and severe techniques Signup and view all the answers

What is the primary difference between the primary response and the response after repeated infection?

The primary response is slower and weaker Signup and view all the answers

What is the main purpose of traditional active immunization?

To prevent infectious diseases Signup and view all the answers

What is the crucial factor in designing a new vaccine?

The understanding of the mechanisms of protective immunity Signup and view all the answers

What is required to prevent tetanus?

A high blood titer of antibody against tetanus toxin Signup and view all the answers

What is the role of adjuvants in vaccine design?

To enhance the immune response Signup and view all the answers

What is the main difference between active and passive immunization?

Active immunization is used for prevention, while passive immunization is used for therapy Signup and view all the answers