Gene Therapy and Vaccines Quiz
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Questions and Answers

What is the primary cause of chronic inflammation of the liver associated with hepatitis B?

  • Hepatitis B virus (correct)
  • Fatty liver disease
  • Bacterial infection
  • Alcohol consumption
  • What was the first hepatitis B vaccine made from?

  • Plasma from infected persons (correct)
  • Yeast cells
  • Recombinant DNA
  • Bacterial cultures
  • What is the main purpose of introducing the Multiple Drug Resistance (MDR) gene into bone marrow cells of cancer patients?

  • To reduce the incidence of cancer recurrence
  • To enhance the immune system's response to chemotherapy
  • To protect bone marrow cells from chemotherapy's toxic effects (correct)
  • To stimulate blood vessel formation in tumors
  • Which method was used to create the recombinant hepatitis B vaccine?

    <p>Inserting viral DNA into yeast cells (C)</p> Signup and view all the answers

    What is the potential advantage of the cholera vaccine produced from genetically engineered potatoes?

    <p>It can elicit an immune response when ingested (D)</p> Signup and view all the answers

    How does anti-angiogenesis gene therapy work as a cancer treatment?

    <p>By reducing blood vessel formation at the tumor site (D)</p> Signup and view all the answers

    What is the objective of therapeutic angiogenesis or VEGF gene therapy?

    <p>To restore and increase blood vessel formation in compromised tissues (D)</p> Signup and view all the answers

    What characterizes the TOMAVAC tomato-based vaccine against COVID-19?

    <p>It expresses the RBD subunit of the S1 protein (D)</p> Signup and view all the answers

    What distinguishes in vivo gene therapy from ex vivo gene therapy?

    <p>In vivo therapy administers genes directly into the body, while ex vivo therapy modifies cells in a lab (B)</p> Signup and view all the answers

    How are vitamins typically synthesized in living organisms?

    <p>Plants and microorganisms synthesize them (A)</p> Signup and view all the answers

    What is a key characteristic of medical-therapeutic agents derived from vitamins?

    <p>They are introduced as treatments beyond their nutritional roles (A)</p> Signup and view all the answers

    What does the vascular endothelial growth factor (VEGF) protein do in therapeutic angiogenesis?

    <p>It induces the formation of new blood vessels to improve oxygen delivery (B)</p> Signup and view all the answers

    What was one of the main challenges with the original method of hepatitis B vaccination?

    <p>High production cost (B)</p> Signup and view all the answers

    What is one key advantage of plant tissue culture techniques in biotechnology?

    <p>It allows for the mass production of identical plant specimens. (A)</p> Signup and view all the answers

    Which of the following describes a disadvantage of genetically modified crops?

    <p>Potential environmental effects and biodiversity loss. (D)</p> Signup and view all the answers

    In the context of plant biotechnology, what is the significance of growth media composition?

    <p>It influences the yield of phytochemicals produced. (B)</p> Signup and view all the answers

    What is one of the learning outcomes related to the enhancement of natural products?

    <p>To analyze methods and elicitation protocols. (B)</p> Signup and view all the answers

    Which methodology is important for the quantitative determination of bioactive metabolites?

    <p>Analytical methods specific to in vitro cultures. (A)</p> Signup and view all the answers

    Which of the following is an expected outcome from student presentations?

    <p>Development of team-working ability through group projects. (C)</p> Signup and view all the answers

    What aspect of plant biotechnology influences the growth regulators' effectiveness?

    <p>The specific growth media composition and environmental conditions. (D)</p> Signup and view all the answers

    How can computer technology benefit students studying plant biotechnology?

    <p>By facilitating access to relevant information and research. (B)</p> Signup and view all the answers

    What is a key benefit of recombinant human insulin compared to animal-sourced insulin?

    <p>It can cause fewer immune responses. (B)</p> Signup and view all the answers

    Which of the following is NOT a general application of medical/pharmaceutical biotechnology?

    <p>Cultivation of crops for food production (B)</p> Signup and view all the answers

    What process allows the introduction of the human insulin gene into bacteria for production?

    <p>Recombinant DNA technology (D)</p> Signup and view all the answers

    Which substance was the first manufactured recombinant pharmaceutical?

    <p>Recombinant human insulin (B)</p> Signup and view all the answers

    How does medical/pharmaceutical biotechnology aim to improve conventional pharmaceuticals?

    <p>By creating safer and more effective versions (B)</p> Signup and view all the answers

    What role do E. coli bacteria play in the production of pharmaceuticals?

    <p>They serve as a gene carrier for hormone production. (A)</p> Signup and view all the answers

    Which of the following is a direct application of pharmaceutical biotechnology?

    <p>Producing insulin (C)</p> Signup and view all the answers

    Which product is NOT typically produced by medical biotechnology?

    <p>Traditional antibiotics (B)</p> Signup and view all the answers

    What is the role of thrombolytic alteplase in medical biotechnology?

    <p>It converts plasminogen to the proteolytic enzyme plasmin. (D)</p> Signup and view all the answers

    Which ingredient is NOT typically found in vaccines?

    <p>Live pathogens (A)</p> Signup and view all the answers

    How do recombinant vaccines differ from conventionally produced vaccines?

    <p>Recombinant vaccines do not carry the risk of infectious contaminants. (D)</p> Signup and view all the answers

    What is necessary for the production of recombinant vaccines?

    <p>Identification and insertion of the target gene into a plasmid. (C)</p> Signup and view all the answers

    What is one benefit of using alteplase over other thrombolytics such as streptokinase?

    <p>It binds to fibrin with a greater affinity. (A)</p> Signup and view all the answers

    What type of microorganisms are typically used in the production of vaccines?

    <p>Nonvirulent microorganisms. (A)</p> Signup and view all the answers

    What triggers the immune system to produce specific antibodies in a vaccinated individual?

    <p>Active ingredients in the vaccine acting as antigens. (D)</p> Signup and view all the answers

    Which of the following steps is involved in the production of recombinant vaccines?

    <p>Introducing plasmids into other living organisms. (C)</p> Signup and view all the answers

    What is the primary purpose of the coli bacterial strain mentioned?

    <p>To produce vitamin B12 without hazardous waste (B)</p> Signup and view all the answers

    What is the first step in the Reichstein–Grüssner process for vitamin C production?

    <p>Hydrogenation of glucose to D-sorbitol (D)</p> Signup and view all the answers

    How is vitamin D primarily synthesized in the human body?

    <p>Through synthesis in the skin via UV light (A)</p> Signup and view all the answers

    What conversion occurs in the liver during vitamin D metabolism?

    <p>Hydroxylation of vitamin D to 25-hydroxyvitamin D (C)</p> Signup and view all the answers

    What role does the Actinomyces hyovaginalis bacterium play in vitamin D metabolism?

    <p>It transforms vitamin D3 into calcitriol (B)</p> Signup and view all the answers

    Which of the following best describes gene therapy?

    <p>Inserting genetic material to treat or cure disease (A)</p> Signup and view all the answers

    What is produced at the final step of the Reichstein–Grüssner process?

    <p>L-ascorbic acid (A)</p> Signup and view all the answers

    What is the second hydroxylation of vitamin D primarily responsible for?

    <p>Creating the active form, 1,25-dihydroxy vitamin D (A)</p> Signup and view all the answers

    Flashcards

    What is plant tissue culture?

    Plant tissue culture is a technique that involves growing plant cells, tissues, or organs in a controlled environment outside of their natural habitat.

    How can plant tissue culture be used for conservation?

    Plant tissue culture can be used to produce large numbers of identical plants, which is useful for propagating rare or endangered species.

    How can plant tissue culture be used for disease control?

    Plant tissue culture can be used to produce disease-free plants, which is important for agriculture and horticulture.

    What is the link between biotechnology and pharmaceutical development?

    Biotechnology can be used to develop new pharmaceuticals by using plant cells to produce valuable compounds.

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    What are genetically modified (GM) crops?

    Genetically modified (GM) crops are plants that have been genetically altered to improve their characteristics, such as yield, pest resistance, or nutrient content.

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    How can genetically modified (GM) crops improve agriculture?

    The use of genetically modified (GM) crops has the potential to improve food security and reduce reliance on pesticides.

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    What are some of the concerns surrounding genetically modified (GM) crops?

    Concerns about the potential environmental and health risks associated with genetically modified (GM) crops are being debated.

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    What is quantitative analysis of bioactive metabolites?

    Quantitative analysis of bioactive metabolites involves using techniques like chromatography and spectroscopy to measure the amounts of active compounds in plants.

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    Medical/Pharmaceutical Biotechnology

    Branch of medicine using cells or cell materials to create pharmaceutical and diagnostic products for treatment or disease prevention.

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    General Applications of Pharmaceutical Biotechnology

    Production of new drugs, safer or more effective versions of existing drugs, or cost-effective alternatives to existing drugs using biotechnology

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    Recombinant Human Insulin

    Using bacteria (like E. coli) to create human insulin, replacing animal-derived insulin.

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    General Applications of Biotechnology

    Biotechnology application that involves the production of hormones, genes, antibiotics, vaccines, interferons, vitamins, and immunological proteins. It can also be used for antenatal diagnosis.

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    Human Recombinant Insulin Production

    An example of a hormone produced by recombinant DNA technology, where the human insulin gene is inserted into bacteria, leading to bacteria producing human insulin.

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    Pharmaceutical Biotechnology and Enzyme Production

    Production of enzymes using biotechnology. These enzymes can be used in various applications.

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    Pharmaceutical Biotechnology and Vaccine Production

    Production of vaccines using biotechnological techniques. This aims to prevent or treat diseases.

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    Pharmaceutical Biotechnology and Vitamins Production

    Production of vitamins using biotechnology. These vitamins are essential for health.

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    Recombinant protein production

    The process of using genetic engineering to modify organisms, often bacteria, to produce large amounts of specific proteins of interest, such as enzymes or antibodies.

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    Amylase

    A type of enzyme that breaks down starch into simpler sugars, used in various industries like food and brewing.

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    Thrombolytic alteplase

    An enzyme that dissolves blood clots, often used to treat heart attacks, strokes, and pulmonary emboli.

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    Vaccination

    The process of inducing immunity against a specific disease by introducing a weakened or inactive form of the pathogen or its components.

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    Recombinant vaccine

    A type of vaccine produced using genetic engineering techniques, where the antigen (the part of the pathogen that triggers an immune response) is produced in a different organism, like bacteria or yeast.

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    Antigen

    A substance that stimulates the immune system to produce specific antibodies.

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    Antibodies

    Proteins produced by the immune system in response to antigens.

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    Recombinant vaccine production for hepatitis B

    A method of producing vaccines using yeast cells that have been genetically modified to express a specific viral antigen. This approach offers advantages like consistency of antigen production and reduced risks associated with traditional methods involving human blood products.

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    Edible vaccines

    A type of vaccine that is delivered orally through food, potentially eliminating the need for injections, improving accessibility, and reducing costs.

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    Potato-based cholera vaccine

    In this approach, the genetic material of a potato plant is modified to produce an antigen, allowing the potato to act as a carrier of the vaccine.

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    Tomato-based COVID-19 vaccine (TOMAVAC)

    This technique involves modifying tomato plants to express the RBD subunit of the SARS-CoV-2 spike protein, making the tomatoes a source of vaccine when eaten.

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    Viral entry mechanism through the spike protein

    This approach utilizes a virus's spike protein to attach to a host cell, triggering an immune response to combat the virus.

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    Vitamins - essential micronutrients

    Essential micronutrients, required by living organisms in small amounts, that play critical roles in metabolism. They are synthesized by microorganisms or plants, not by animals.

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    Medical applications of vitamins

    Increasingly, vitamins are being used not only as supplements to support nutrition but also as therapeutic agents for specific medical conditions.

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    Biotechnology in vitamin production

    Biotechnology, specifically genetic engineering, is used in the production of vitamins, leveraging the ability of microorganisms or plant cells to synthesize these essential compounds.

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    In vivo gene therapy

    A type of gene therapy where the therapeutic gene is directly delivered into the patient's body, often using a vector like a virus.

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    Ex vivo gene therapy

    A type of gene therapy where cells are removed from the patient, modified with the therapeutic gene in a lab, and then returned to the patient.

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    Anti-angiogenesis gene therapy

    A gene therapy strategy that aims to prevent or treat cancer by stopping the growth of blood vessels that supply tumors with nutrients.

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    Therapeutic angiogenesis

    A gene therapy strategy that aims to stimulate the growth of blood vessels to treat conditions like sensory neuropathy or critical limb ischemia.

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    Multidrug resistance (MDR) gene therapy

    A gene therapy approach that introduces the MDR gene into bone marrow cells to protect them from the toxic effects of chemotherapy drugs, making high-dose chemotherapy safer and improving recovery.

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    What is the significance of the coli bacterial strain mentioned?

    A bacterial strain that produces high levels of vitamin B12 while reducing harmful waste and making the manufacturing process more cost-effective and eco-friendly. This strain offers a reliable alternative for commercial partners when vitamin B12 costs become excessive.

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    Explain the industrial process for producing Vitamin C.

    Reichstein-Grüssner process is the industrial production of vitamin C, starting with glucose. This involves hydrogenation to D-sorbitol, oxidation to L-sorbose using Acetobacter suboxydans or Gluconobacter oxydans, and finally chemical conversion to L-ascorbic acid.

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    Explain the synthesis of vitamin D.

    Vitamin D is produced in the skin by UV light exposure or through dietary intake. Skin's 7-dehydrocholesterol reacts with UVB radiation to form previtamin D3. In the liver, vitamin D is converted to 25-hydroxyvitamin D (calcidiol). Finally, in the kidney, the active form 1,25-dihydroxyvitamin D (calcitriol) is produced.

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    How can Actinomyces hyovaginalis be used in Vitamin D production?

    Actinomyces hyovaginalis is a bacterium capable of converting vitamin D3 into calcitriol. This process offers a potential solution for producing active vitamin D3 for individuals with impaired kidney function, who struggle to produce it naturally.

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    What is Gene Therapy?

    Gene therapy involves delivering genetic material into cells to treat or prevent diseases. This can involve repairing defective genes or making tumors more vulnerable to other therapies. The delivery can be achieved through different methods.

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    How can gene therapy be delivered?

    Gene therapy can be delivered into cells in two main ways.

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    Study Notes

    Biotechnological Production of Herbal Drugs [PPC505]

    • Syllabus covers advising, registration, biotechnology, plant tissue culture, plant genetic manipulation, pharmaceutical applications, student presentations and exams.
    • Weeks 1-15 are outlined, detailing specific topics and dates for lectures, midterm exams, and final exams.
    • Learning outcomes include describing biotechnology types and importance, recognizing plant tissue culture principles, illustrating production concepts for pharmaceuticals, demonstrating the relationship between growth media and yield, describing genetically modified crops, and explaining different analytical methods for bioactive metabolites.
    • Further learning outcomes include assessment of experimental schemes, analysis of methods and elicitation protocols for natural product production, interpretation of transgenic plant protocols, development of team-working and presentation skills, and computer technology use.
    • The agenda focuses on plant biotechnology and pharmaceutical applications of biotechnology.
    • General applications of modern biotechnology in the pharmaceutical field, encompassing creation of new pharmaceuticals, safer versions of existing pharmaceuticals, and cost-effective production of similar substances.
    • Recombinant human insulin is highlighted as a prime example, contrasting it with animal insulin's differences .
    • Modern biotechnology involves hormone, gene, antibiotic, vaccine, interferon, vitamin production, as well as antenatal diagnosis for preventing genetic diseases.
    • Specific applications include pharmaceutical biotechnology and hormone production, enzyme production, vaccine production, vitamin production, and gene therapy.
    • Human recombinant insulin is the first human hormone produced through recombinant DNA technology, involving gene insertion into plasmids and transferring them into bacteria (E. coli or Saccharomyces cerevisiae).
    • Insulin lispro is a genetically modified human insulin with peptide structure modifications affecting its action.
    • Erythropoietin (EPO) regulates red blood cell production and is used for anemia treatment.
    • Human growth hormone (hGH) is vital for growth, and recombinant hGH has replaced the use of cadaver-derived hormone.
    • Amylase has various industrial and digestive applications, involving recombinant DNA technology.
    • Thrombolytic enzyme (Alteplase) dissolves blood clots in the circulatory system.
    • Vaccination is an effective disease control tool mimicking natural specific defense mechanism for pathogen resistance.
    • Vaccines use killed microorganisms, non-virulent microorganisms, and microbial components.
    • Recombinant vaccines are produced by inserting the gene into suitable carriers and expressing/purifying the material.
    • Hepatitis B is a significant global health concern
    • Genetically engineered potatoes for cholera vaccine production (TOMAVAC) illustrate edible vaccines like a tomato based edible vaccine for Covid-19.
    • Vitamins are essential micronutrients for metabolism in living organisms, synthesized naturally and can also be produced through biotechnology.
    • Vitamin production through chemical synthesis has environmental concerns whereas microbial methods are safer and more sustainable.
    • Vitamins B12 and C are produced via fermentation from microorganisms like Pseudomonas denitrificans, Propionibacterium freudenreichii and Acetobacter suboxydans/Gluconobacter oxydans respectively, requiring various purifying steps.
    • Vitamin D is synthesized in skin using UV light or ingested from dietary sources.
    • Gene therapy involves inserting genetic material into cells for disease prevention, control, or cure; two main methods exist: in vivo and ex vivo.
    • Multidrug resistance (MDR) gene therapy for cancer and anti-angiogenesis gene therapy to decrease tumor angiogenesis are highlighted.
    • Future lectures will include student presentations about plant biotechnology.

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    Test your knowledge on gene therapy techniques and vaccine development, including topics such as hepatitis B vaccines and anti-angiogenesis strategies in cancer treatment. This quiz delves into the scientific principles behind various medical therapies and biotechnology innovations. Explore the advances in genetic engineering and their implications for health.

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