Gene Regulation Overview

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Questions and Answers

Which molecule acts as a regulatory protein to inhibit gene expression?

  • enhancer
  • repressor (correct)
  • activator
  • transcription factor

Which molecule promotes the repression of gene expression by combining with a repressor?

  • metabolite
  • promoter
  • corepressor (correct)
  • inducer

What is the function of an inducer in gene regulation?

  • binds to RNA polymerase
  • alters repressor function (correct)
  • activates the transcription process
  • promotes degradation of mRNA

What would be the consequence of deactivating a regulatory gene in a repressible operon?

<p>permanent transcription of the structural gene (A)</p> Signup and view all the answers

Which of the following best describes a situation where the lac operon is likely to be transcribed?

<p>low levels of glucose and high levels of lactose (B)</p> Signup and view all the answers

What is the role of cAMP in bacterial gene regulation?

<p>activates transcription factors (B)</p> Signup and view all the answers

Which of the following repressions occurs when an operator is blocked by a repressor?

<p>negative control (C)</p> Signup and view all the answers

What effect does a corepressor have on a repressor and the associated operon?

<p>decreases transcription by enhancing repressor binding (D)</p> Signup and view all the answers

What is a potential effect of decreased chromatin condensation in eukaryotic gene expression?

<p>Inactivation of the gene (C)</p> Signup and view all the answers

Which method is uniquely employed by eukaryotes for gene expression regulation?

<p>Regulatory proteins binding to promoter sequences (A)</p> Signup and view all the answers

What is the primary function of RNA interference in eukaryotic cells?

<p>Destroying RNA with complementary sequences (C)</p> Signup and view all the answers

Which type of DNA is found in the noncoding regions of the human genome?

<p>Transcribed into small RNAs with biological functions (A)</p> Signup and view all the answers

What role does piRNA play during gamete formation?

<p>Reestablishing methylation patterns (B)</p> Signup and view all the answers

Which statement accurately characterizes siRNA?

<p>It is involved in RNA degradation. (B)</p> Signup and view all the answers

What effect does regulatory protein binding to promoter sequences have in eukaryotic gene expression?

<p>Transcriptional activation or repression (A)</p> Signup and view all the answers

Which characteristic distinguishes eukaryotic gene regulation from bacterial regulation?

<p>The presence of introns in genes (C)</p> Signup and view all the answers

What is the primary method to determine the amount of mRNA generated from a gene?

<p>Assess the amount of the mRNA generated (C)</p> Signup and view all the answers

Which method would be the most effective in analyzing alternative splicing of a gene?

<p>Isolating mRNA from the gene and analyzing the sequences (C)</p> Signup and view all the answers

What is a common mechanism for coordinating the expression of multiple, related genes in eukaryotic cells?

<p>Specific combinations of control elements for co-expressed genes (B)</p> Signup and view all the answers

DNA methylation and histone acetylation are examples of which type of biochemical processes?

<p>Epigenetic modifications (C)</p> Signup and view all the answers

Which role is typically associated with general transcription factors in eukaryotic cells?

<p>Recruiting other proteins to the promoter region (C)</p> Signup and view all the answers

Which of the following does NOT contribute to the regulation of eukaryotic gene expression?

<p>Direct modification of the coding sequence of the gene (A)</p> Signup and view all the answers

What is the main consequence of alternative splicing in gene expression?

<p>Production of multiple protein isoforms from a single gene (A)</p> Signup and view all the answers

Which of the following describes a common outcome of histone acetylation on gene activity?

<p>Enhanced accessibility of the DNA for transcription (C)</p> Signup and view all the answers

What is the role of tryptophan in the functioning of the repressor in bacteria?

<p>It causes the repressor to remain bound to the operator. (A)</p> Signup and view all the answers

How does an increase in intracellular cAMP influence the transcription of the lac operon?

<p>It promotes the binding of the cAMP receptor protein to the promoter. (B)</p> Signup and view all the answers

What is the effect of glucose on the lac operon?

<p>Glucose presence leads to decreased concentrations of cAMP. (B)</p> Signup and view all the answers

What happens to the cAMP receptor protein (CRP) as extracellular glucose levels rise?

<p>CRP detaches from the lac promoter, reducing transcription. (A)</p> Signup and view all the answers

In what way does the binding of the cAMP receptor protein (CRP) promote the lac operon transcription?

<p>By facilitating binding to the CRP-binding site near the promoter. (B)</p> Signup and view all the answers

What is the consequence of a yeast mutant with histones resistant to acetylation?

<p>The mutant has decreased accessibility of DNA for transcription. (A)</p> Signup and view all the answers

Which statement best describes the relationship between lactose and the lac operon?

<p>Lactose prevents the lac repressor from inhibiting transcription. (A)</p> Signup and view all the answers

What would be the expected outcome if RNA polymerase cannot open the DNA strands at the lac operon?

<p>Transcription of the lac operon would be inhibited. (C)</p> Signup and view all the answers

Which characteristic accurately describes tumor-suppressor genes?

<p>They encode proteins that help prevent uncontrolled cell growth. (B)</p> Signup and view all the answers

What function do the normal products of BRCA1 and BRCA2 serve?

<p>They participate in repair of DNA damage. (C)</p> Signup and view all the answers

What effect do mutant forms of Ras protein typically have in tumors?

<p>They lead to excessive cell division. (C)</p> Signup and view all the answers

Which type of cancer is primarily associated with mutations in the APC gene?

<p>Colorectal cancer. (A)</p> Signup and view all the answers

What role do tumor-suppressor genes generally perform in cell regulation?

<p>They help in controlling and inhibiting cell growth. (A)</p> Signup and view all the answers

What is the primary outcome of deregulated Ras protein activity?

<p>Increased cell signaling for growth. (B)</p> Signup and view all the answers

In what capacity do tumor-suppressor genes interact with proto-oncogenes?

<p>They serve to inhibit proto-oncogene activation. (C)</p> Signup and view all the answers

What is a significant characteristic of cancer that results from genetic changes in cell cycle control?

<p>It involves mutations in specific genes regulating cell division. (C)</p> Signup and view all the answers

What is the role of the MyoD protein in cellular differentiation?

<p>It causes undifferentiated fat cells to become muscle cells. (C)</p> Signup and view all the answers

Which statement best describes cytoplasmic determinants?

<p>They are substances from the maternal egg that guide early development. (B)</p> Signup and view all the answers

Why does the introduction of MyoD into neurons fail to produce muscle cells?

<p>MyoD is not effective in cells that are already highly differentiated. (C)</p> Signup and view all the answers

What type of gene expression is primarily influenced by the bicoid gene in Drosophila?

<p>Body patterning during early embryonic development. (D)</p> Signup and view all the answers

What distinguishes cytoplasmic determinants from other types of regulatory molecules in embryos?

<p>They have a maternal origin and influence early development. (A)</p> Signup and view all the answers

How does MyoD influence gene expression in fat cells compared to neurons?

<p>Fat cells recognize MyoD due to their less differentiated state. (B)</p> Signup and view all the answers

What characterizes the differentiated state of cells?

<p>It is often very unstable and can lead to cell death. (C)</p> Signup and view all the answers

Which of the following options correctly explains the role of MyoD in muscle differentiation?

<p>MyoD is a transcription factor that activates muscle-specific gene expression. (B)</p> Signup and view all the answers

Flashcards

Lac Operon Repressor Binding

The lac operon repressor binds to the operator region of the DNA, preventing RNA polymerase from transcribing the lac genes when lactose is absent.

Glucose Breakdown and Lac Operon

Breakdown of glucose inhibits lac operon transcription by reducing intracellular cAMP levels, causing the CRP to detach from the promoter, thus weakening transcription.

Extracellular Glucose and Lac Operon

An increase in extracellular glucose weakens the binding of CRP to the lac promoter, reducing the rate of transcription of the lac operon.

CRP and Positive Regulation

CRP (cAMP receptor protein) positively regulates the lac operon by binding to the CRP-binding site and stimulating lac operon transcription.

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Acetylation Resistant Histones

Yeast mutants with histones resistant to acetylation experience decreased levels of gene expression.

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Repressor protein

A protein produced by a regulatory gene that binds to an operator to block transcription.

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Operator

A segment of DNA where a repressor protein binds, controlling transcription.

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Inducer

A molecule that binds to a repressor, causing it to change shape and no longer bind to the operator.

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Corepressor

A molecule that binds to a repressor, causing it to change shape and bind to the operator, thereby stopping transcription.

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Lac operon

A group of genes in bacteria that are involved in lactose metabolism.

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Regulatory gene

A gene that codes for a protein involved in controlling the expression of other genes.

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Mutation in regulatory gene (repressible operon)

A change in a regulatory gene that results in continuous transcription of the structural gene.

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Conditions for lac operon transcription

Transcription of the lac operon is most likely to occur when lactose is present and glucose is scarce.

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MyoD protein effect on cell differentiation

The MyoD protein triggers differentiation of fat cells into muscle cells, but has no effect on neurons due to different sets of required genes activated.

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Differentiation instability

Differentiated states often revert easily.

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Cytoplasmic determinants role

Cytoplasmic determinants are 'maternal' components in the egg affecting development.

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Drosophila bicoid gene product function

The bicoid gene product in Drosophila establishes the anterior-posterior axis during development.

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Eukaryotic gene expression regulation

The control of gene expression in eukaryotic cells, which occurs at multiple stages, including transcription, mRNA processing, and translation

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Tumor Suppressor Genes

Genes that produce proteins preventing uncontrolled cell growth.

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Alternative splicing

A process where a single gene can produce multiple protein isoforms from a single primary transcript by varying the exons included in the mature mRNA.

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BRCA1 and BRCA2

Tumor suppressor genes involved in repairing DNA damage.

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mRNA amount

In a gene, a determinant factor for how much protein is produced.

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Ras Protein (tumors)

Forms of Ras protein in tumors lead to excessive cell division.

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APC gene

A gene involved in colorectal cancer predisposition.

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Alternative Splicing Detection

Determining if a gene undergoes alternative splicing by comparing mRNA sequences.

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Cancer Cell Cycle Control

Cancer is caused by genetic changes affecting the mechanisms regulating cell division.

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Coordinating gene expression

Multiple related genes being expressed together, regulated by combinations of control elements.

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Epigenetic phenomena

Heritable changes in gene expression that do not involve changes to the underlying DNA sequence, such as DNA methylation and histone modification.

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General transcription factors function

Proteins that bind to the DNA and assemble the transcription initiation complex, allowing RNA polymerase to bind the promoter and begin transcription.

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Eukaryotic Gene Expression Regulation

Eukaryotes control gene activity through methods beyond those used by bacteria, including RNA splicing and chromatin remodeling.

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RNA Interference (RNAi)

A process where introduced double-stranded RNA destroys RNA molecules with complementary sequences.

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Non-coding DNA

Non-protein-coding DNA regions in the genome, often transcribed into small RNAs.

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piRNA

A type of small RNA involved in re-establishing methylation patterns during gamete formation.

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siRNA

Short interfering RNA; specific small interfering RNAs that can be involved in controlling gene expression.

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Chromatin Remodeling

Processes affecting the structure of chromatin (DNA-protein complex) to control gene access.

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Histone Acetylation

The enzymatic modification affecting the chromatin structure affecting gene expression. Histone acetylation is used by eukaryotes to regulate gene expression.

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Study Notes

Gene Regulation

  • Gene regulation is the process of controlling when and how genes are expressed.
  • This process is important for all organisms.

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