17 Questions
What is the term for a strain of bacteria that is temporarily partially diploid due to the insertion of F factors via conjugation?
Merozygote
In a partial diploid bacterial genotype with the alleles I-/I+, what is the resulting phenotype?
Wildtype phenotype
What type of mutation is the 'super repressor' described in the text?
Dominant negative
Why can linear pieces of DNA added to bacteria not be replicated within bacterial cells?
Linear DNA lacks ORI
What genetic element can be taken up by bacteria through conjugation, and is described as small circular pieces of DNA?
F- factors
In a merozygote with the genotype I+O+Z+Y+/IsO+Z+Y+, what is the resulting phenotype?
Constitutive phenotype
Why won't a eukaryotic gene sequence be properly expressed in bacteria?
Bacteria lack splicesomes to remove introns from the gene sequence.
What is a possible reason for the lack of colonies on a plate after attempting gene transformation?
The transformation process was unsuccessful.
What is the purpose of using X-gal when testing for the insertion of an external gene in a plasmid?
X-gal confirms if a gene insert disrupts the lacz gene within the plasmid.
Why do colonies remain white on an agar plate when the lacz gene is not disrupted?
The lacz gene produces a blue pigment under specific circumstances.
What role does the antibiotic resistance gene play in identifying successful plasmid transformation?
Antibiotic resistance genes ensure only genetically modified bacteria survive and grow on plates.
What is the critical step in prokaryotic gene expression regulation?
Transcription initiation
What is the role of a repressor in a repressible operon like the trp operon?
It binds to the operator to inhibit transcription
What is the default state of an inducible operon like the lac operon?
Off
What is the inducer of the lac operon in bacteria?
IPTG
In prokaryotic gene expression, what is the binding site for a repressor protein called?
Operator
Which type of genes encode enzymes for the synthesis of tryptophan in prokaryotes?
Repressible genes
Test your knowledge on gene expression in bacteria transformation process. Learn about the importance of pre-spliced transcripts, plasmid presence, and using X-gal to detect inserted genes. Understand the significance of introns, splicesomes, and antibiotic resistance genes.
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