Podcast
Questions and Answers
What is the term for a strain of bacteria that is temporarily partially diploid due to the insertion of F factors via conjugation?
What is the term for a strain of bacteria that is temporarily partially diploid due to the insertion of F factors via conjugation?
- Conjugated bacteria
- Haploid bacteria
- Merozygote (correct)
- Transgenic bacteria
In a partial diploid bacterial genotype with the alleles I-/I+, what is the resulting phenotype?
In a partial diploid bacterial genotype with the alleles I-/I+, what is the resulting phenotype?
- Wildtype phenotype (correct)
- Mixed phenotype
- Mutant phenotype
- Unknown phenotype
What type of mutation is the 'super repressor' described in the text?
What type of mutation is the 'super repressor' described in the text?
- Loss of function only
- Neutral mutation
- Dominant negative (correct)
- Gain of function only
Why can linear pieces of DNA added to bacteria not be replicated within bacterial cells?
Why can linear pieces of DNA added to bacteria not be replicated within bacterial cells?
What genetic element can be taken up by bacteria through conjugation, and is described as small circular pieces of DNA?
What genetic element can be taken up by bacteria through conjugation, and is described as small circular pieces of DNA?
In a merozygote with the genotype I+O+Z+Y+/IsO+Z+Y+, what is the resulting phenotype?
In a merozygote with the genotype I+O+Z+Y+/IsO+Z+Y+, what is the resulting phenotype?
Why won't a eukaryotic gene sequence be properly expressed in bacteria?
Why won't a eukaryotic gene sequence be properly expressed in bacteria?
What is a possible reason for the lack of colonies on a plate after attempting gene transformation?
What is a possible reason for the lack of colonies on a plate after attempting gene transformation?
What is the purpose of using X-gal when testing for the insertion of an external gene in a plasmid?
What is the purpose of using X-gal when testing for the insertion of an external gene in a plasmid?
Why do colonies remain white on an agar plate when the lacz gene is not disrupted?
Why do colonies remain white on an agar plate when the lacz gene is not disrupted?
What role does the antibiotic resistance gene play in identifying successful plasmid transformation?
What role does the antibiotic resistance gene play in identifying successful plasmid transformation?
What is the critical step in prokaryotic gene expression regulation?
What is the critical step in prokaryotic gene expression regulation?
What is the role of a repressor in a repressible operon like the trp operon?
What is the role of a repressor in a repressible operon like the trp operon?
What is the default state of an inducible operon like the lac operon?
What is the default state of an inducible operon like the lac operon?
What is the inducer of the lac operon in bacteria?
What is the inducer of the lac operon in bacteria?
In prokaryotic gene expression, what is the binding site for a repressor protein called?
In prokaryotic gene expression, what is the binding site for a repressor protein called?
Which type of genes encode enzymes for the synthesis of tryptophan in prokaryotes?
Which type of genes encode enzymes for the synthesis of tryptophan in prokaryotes?