Gene Expression in Bacteria Transformation Quiz
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Questions and Answers

What is the term for a strain of bacteria that is temporarily partially diploid due to the insertion of F factors via conjugation?

  • Conjugated bacteria
  • Haploid bacteria
  • Merozygote (correct)
  • Transgenic bacteria

In a partial diploid bacterial genotype with the alleles I-/I+, what is the resulting phenotype?

  • Wildtype phenotype (correct)
  • Mixed phenotype
  • Mutant phenotype
  • Unknown phenotype

What type of mutation is the 'super repressor' described in the text?

  • Loss of function only
  • Neutral mutation
  • Dominant negative (correct)
  • Gain of function only

Why can linear pieces of DNA added to bacteria not be replicated within bacterial cells?

<p>Linear DNA lacks ORI (D)</p> Signup and view all the answers

What genetic element can be taken up by bacteria through conjugation, and is described as small circular pieces of DNA?

<p>F- factors (B)</p> Signup and view all the answers

In a merozygote with the genotype I+O+Z+Y+/IsO+Z+Y+, what is the resulting phenotype?

<p>Constitutive phenotype (C)</p> Signup and view all the answers

Why won't a eukaryotic gene sequence be properly expressed in bacteria?

<p>Bacteria lack splicesomes to remove introns from the gene sequence. (C)</p> Signup and view all the answers

What is a possible reason for the lack of colonies on a plate after attempting gene transformation?

<p>The transformation process was unsuccessful. (C)</p> Signup and view all the answers

What is the purpose of using X-gal when testing for the insertion of an external gene in a plasmid?

<p>X-gal confirms if a gene insert disrupts the lacz gene within the plasmid. (B)</p> Signup and view all the answers

Why do colonies remain white on an agar plate when the lacz gene is not disrupted?

<p>The lacz gene produces a blue pigment under specific circumstances. (C)</p> Signup and view all the answers

What role does the antibiotic resistance gene play in identifying successful plasmid transformation?

<p>Antibiotic resistance genes ensure only genetically modified bacteria survive and grow on plates. (A)</p> Signup and view all the answers

What is the critical step in prokaryotic gene expression regulation?

<p>Transcription initiation (B)</p> Signup and view all the answers

What is the role of a repressor in a repressible operon like the trp operon?

<p>It binds to the operator to inhibit transcription (C)</p> Signup and view all the answers

What is the default state of an inducible operon like the lac operon?

<p>Off (B)</p> Signup and view all the answers

What is the inducer of the lac operon in bacteria?

<p>IPTG (D)</p> Signup and view all the answers

In prokaryotic gene expression, what is the binding site for a repressor protein called?

<p>Operator (C)</p> Signup and view all the answers

Which type of genes encode enzymes for the synthesis of tryptophan in prokaryotes?

<p>Repressible genes (B)</p> Signup and view all the answers

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