Gene Expression in Bacteria Transformation Quiz
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Questions and Answers

What is the term for a strain of bacteria that is temporarily partially diploid due to the insertion of F factors via conjugation?

  • Conjugated bacteria
  • Haploid bacteria
  • Merozygote (correct)
  • Transgenic bacteria
  • In a partial diploid bacterial genotype with the alleles I-/I+, what is the resulting phenotype?

  • Wildtype phenotype (correct)
  • Mixed phenotype
  • Mutant phenotype
  • Unknown phenotype
  • What type of mutation is the 'super repressor' described in the text?

  • Loss of function only
  • Neutral mutation
  • Dominant negative (correct)
  • Gain of function only
  • Why can linear pieces of DNA added to bacteria not be replicated within bacterial cells?

    <p>Linear DNA lacks ORI</p> Signup and view all the answers

    What genetic element can be taken up by bacteria through conjugation, and is described as small circular pieces of DNA?

    <p>F- factors</p> Signup and view all the answers

    In a merozygote with the genotype I+O+Z+Y+/IsO+Z+Y+, what is the resulting phenotype?

    <p>Constitutive phenotype</p> Signup and view all the answers

    Why won't a eukaryotic gene sequence be properly expressed in bacteria?

    <p>Bacteria lack splicesomes to remove introns from the gene sequence.</p> Signup and view all the answers

    What is a possible reason for the lack of colonies on a plate after attempting gene transformation?

    <p>The transformation process was unsuccessful.</p> Signup and view all the answers

    What is the purpose of using X-gal when testing for the insertion of an external gene in a plasmid?

    <p>X-gal confirms if a gene insert disrupts the lacz gene within the plasmid.</p> Signup and view all the answers

    Why do colonies remain white on an agar plate when the lacz gene is not disrupted?

    <p>The lacz gene produces a blue pigment under specific circumstances.</p> Signup and view all the answers

    What role does the antibiotic resistance gene play in identifying successful plasmid transformation?

    <p>Antibiotic resistance genes ensure only genetically modified bacteria survive and grow on plates.</p> Signup and view all the answers

    What is the critical step in prokaryotic gene expression regulation?

    <p>Transcription initiation</p> Signup and view all the answers

    What is the role of a repressor in a repressible operon like the trp operon?

    <p>It binds to the operator to inhibit transcription</p> Signup and view all the answers

    What is the default state of an inducible operon like the lac operon?

    <p>Off</p> Signup and view all the answers

    What is the inducer of the lac operon in bacteria?

    <p>IPTG</p> Signup and view all the answers

    In prokaryotic gene expression, what is the binding site for a repressor protein called?

    <p>Operator</p> Signup and view all the answers

    Which type of genes encode enzymes for the synthesis of tryptophan in prokaryotes?

    <p>Repressible genes</p> Signup and view all the answers

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