Podcast
Questions and Answers
What prevents an mRNA from existing in the cell forever and therefore being translated forever?
What prevents an mRNA from existing in the cell forever and therefore being translated forever?
- Methylation of the mRNA
- Inhibition by microRNAs
- Binding to histones
- Degradation by ribonucleases (correct)
How can a microRNA stop translation of a specific mRNA, but not all mRNAs in the cell?
How can a microRNA stop translation of a specific mRNA, but not all mRNAs in the cell?
- By base pairing with the specific mRNA's 3' UTR (correct)
- By interacting with the general transcription factors
- By binding to the mRNA's promoter region
- By altering the mRNA's 5' UTR
What are the advantages or disadvantages for a eukaryote to regulate the amount or activity of a protein in a cell via transcriptional control vs. post-translational control?
What are the advantages or disadvantages for a eukaryote to regulate the amount or activity of a protein in a cell via transcriptional control vs. post-translational control?
- Transcriptional control allows for long-term regulation, while post-translational control provides rapid response. (correct)
- Post-translational control affects only specific proteins, while transcriptional control affects all proteins.
- Post-translational control allows for long-term regulation, while transcriptional control provides rapid response.
- Transcriptional control affects only specific proteins, while post-translational control affects all proteins.
In eukaryotes, why must transcription be complete before translation begins?
In eukaryotes, why must transcription be complete before translation begins?
If a strain of E. coli has a mutation in the operator of the trp operon that prevents binding by a repressor, will the operon be transcribed in the presence of tryptophan?
If a strain of E. coli has a mutation in the operator of the trp operon that prevents binding by a repressor, will the operon be transcribed in the presence of tryptophan?
If a strain of E. coli has a mutation in the operator of the trp operon, resulting in the repressor binding all the time even in the absence of tryptophan, what will happen?
If a strain of E. coli has a mutation in the operator of the trp operon, resulting in the repressor binding all the time even in the absence of tryptophan, what will happen?
If a strain of E. coli has a mutation so that no repressor protein is made, will transcription occur in the presence of lactose if glucose levels are low?
If a strain of E. coli has a mutation so that no repressor protein is made, will transcription occur in the presence of lactose if glucose levels are low?
What is the function of a microRNA in stopping translation of a specific mRNA?
What is the function of a microRNA in stopping translation of a specific mRNA?
What is the role of an operator in the regulation of gene expression?
What is the role of an operator in the regulation of gene expression?
What is the primary function of an enhancer in gene regulation?
What is the primary function of an enhancer in gene regulation?
In eukaryotes, why must transcription be complete before translation begins?
In eukaryotes, why must transcription be complete before translation begins?
If a strain of E. coli has a mutation in the operator of the trp operon that results in the repressor binding all the time even in the absence of tryptophan, what will happen?
If a strain of E. coli has a mutation in the operator of the trp operon that results in the repressor binding all the time even in the absence of tryptophan, what will happen?
Explain why alternative splicing can never change the order of exons in an mRNA transcript.
Explain why alternative splicing can never change the order of exons in an mRNA transcript.
What is the difference between a general transcription factor and a specific transcription factor?
What is the difference between a general transcription factor and a specific transcription factor?
What prevents an mRNA from existing in the cell forever and therefore being translated forever?
What prevents an mRNA from existing in the cell forever and therefore being translated forever?
How can a microRNA stop translation of a specific mRNA, but not all mRNAs in the cell?
How can a microRNA stop translation of a specific mRNA, but not all mRNAs in the cell?
What is the role of an operator in the regulation of gene expression?
What is the role of an operator in the regulation of gene expression?
In eukaryotes, why must transcription be complete before translation begins?
In eukaryotes, why must transcription be complete before translation begins?
If a strain of E. coli has a mutation in the operator of the trp operon that prevents binding by a repressor, will the operon be transcribed in the presence of tryptophan?
If a strain of E. coli has a mutation in the operator of the trp operon that prevents binding by a repressor, will the operon be transcribed in the presence of tryptophan?
If a strain of E. coli has a mutation in the operator of the trp operon that results in the repressor binding all the time even in the absence of tryptophan, explain what will happen in the presence and absence of tryptophan.
If a strain of E. coli has a mutation in the operator of the trp operon that results in the repressor binding all the time even in the absence of tryptophan, explain what will happen in the presence and absence of tryptophan.
Explain why alternative splicing can never change the order of exons in an mRNA transcript.
Explain why alternative splicing can never change the order of exons in an mRNA transcript.
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