RNA Interference Quiz

RNA interference limits the level of transcript in two ways: by suppressing transcription (Transcriptional gene silencing) and degrading the RNA produced (post-transcriptional gene silencing).

Andrew Z. Fire and Craig C. Mello discovered the process of RNA interference in the cells of the nematode.

RNA interference is the process in which gene expression is inhibited by RNA molecules that neutralize targeted mRNA molecules. It is an evolutionarily conserved mechanism triggered by double-stranded RNA that uses the gene's own DNA sequence to turn it off, known as gene silencing.

<p>The steps involved in the mechanism of RNA interference include dicing long double-stranded RNA into small fragments with the help of an enzyme called Dicer, passing the small fragments through the RNA-induced silencing complex, unwinding the duplex, activating the RNA, promoting RNA degradation and inhibiting translation, binding the siRNA to the Argonaute protein, removing one of the strands from the double-stranded siRNA, and the remaining strand binding to the mRNA target sequences. The Argonaute protein either cleaves the mRNA or recruits other factors to regulate the target sequence.</p> Signup and view all the answers

<p>The siRNA, or small interfering RNA, is involved in promoting RNA degradation and inhibiting translation by binding to the Argonaute protein and targeting mRNA sequences for cleavage or regulation.</p> Signup and view all the answers

<p>Dicer dices long double-stranded RNA into small fragments known as siRNA or small interfering RNA.</p> Signup and view all the answers

<p>The RNA-induced silencing complex promotes RNA degradation and inhibits translation of RNA.</p> Signup and view all the answers

<p>The siRNA binds to Argonaute protein, and one of the strands from the double-stranded siRNA is removed, allowing the remaining strand to bind to the mRNA target sequences.</p> Signup and view all the answers

<p>The Argonaute protein either cleaves the mRNA or recruits other factors to regulate the target sequence.</p> Signup and view all the answers

<p>RNA interference limits the level of transcript by suppressing transcription (transcriptional gene silencing) and degrading the RNA produced (post-transcriptional gene silencing).</p> Signup and view all the answers