Gene Expression Analysis

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What is the primary goal of the GTEx project?

To identify genetic mutations associated with diseases
To catalog gene expression across different human tissues (correct)
To develop a new generation of microarrays
To study the mechanism of RNA-seq

What is the minimum recommended number of biological replicates for microarray analysis?

n≥5 per group
n≥3 per group (correct)
n≥2 per group
n≥4 per group

What is the purpose of Stage 3 in the microarray analysis process?

Experimental design
Microarray data analysis
RNA extraction and conversion
Hybridization to DNA arrays (correct)

What type of probes are used in microarray analysis?

Oligonucleotide probes (C) Signup and view all the answers

What is the purpose of clustering in microarray data analysis?

To find meaningful patterns in the data (B) Signup and view all the answers

What is the primary difference between microarray analysis and RNA-seq analysis?

The technology used to measure gene expression (C) Signup and view all the answers

What is a possible reason for weak correlations in gene expression studies?

All of the above (D) Signup and view all the answers

What percentage of the human genome is spanned by processed or primary transcripts, respectively?

62.1% and 74.7% (A) Signup and view all the answers

What is a characteristic of coding RNA transcripts compared to noncoding transcripts?

Coding RNA transcripts tend to be cytosolic (C) Signup and view all the answers

What is a type of quantitative trait that can be described for a given cell type and physiological state in an organism?

mRNA expression (B) Signup and view all the answers

What can impact mRNA expression, according to the text?

Variants in genomic DNA (A) Signup and view all the answers

What is the approximate percentage of annotated coding and noncoding transcripts that overlap small noncoding RNAs?

6% (B) Signup and view all the answers

What is the primary function of expression quantitative trait loci (eQTLs)?

To control expression levels of specific genes (C) Signup and view all the answers

What is the main difference between cis-eQTLs and trans-eQTLs?

Cis-eQTLs act on transcripts from neighboring genes, while trans-eQTLs act on distant genes (D) Signup and view all the answers

How do eQTLs affect transcription?

By altering the sequence of a transcription factor binding site (C) Signup and view all the answers

What is the focus of the field of functional genomics?

The high-throughput study of gene expression in all organisms (C) Signup and view all the answers

What is the main advantage of functional genomics over traditional gene expression studies?

It allows for the study of the expression of entire genomes (C) Signup and view all the answers

What is the ultimate goal of studying gene expression in functional genomics?

To understand the transcriptional program of entire genomes (A) Signup and view all the answers