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Questions and Answers
What does the term 'transcriptome' refer to?
What is the main purpose of RNAseq (or RNA-seq)?
What types of reads are obtained from RNA-Seq?
What is the read length range for RNA-Seq reads?
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Which types of RNA are mainly studied using RNA-seq?
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What is the purpose of transcriptome sequencing?
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What is one of the crucial factors in RNAseq?
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What is recommended regarding outliers with over 30% disagreement in raw reads?
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What does the optimal library size depend on in RNAseq?
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Which type of reads are preferable to characterize poorly annotated transcriptomes in RNA-seq?
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What is one of the quality-control checkpoints for raw reads in RNA-seq?
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What varies with effect size, sequencing depth, and number of replicates in RNA-seq?
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Which step involves obtaining raw reads, read alignment, and quantification in RNA-seq data acquisition?
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What is crucial for proper sequencing and subsequent analysis in RNA-seq?
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What should be homogeneous for samples in the same experiments in RNA-seq?
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Which one of these is true about RNA-seq?
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