16 Questions
What does the term 'transcriptome' refer to?
The collection of all transcripts, including mRNA, rRNA, tRNA, and ncRNA
What is the main purpose of RNAseq (or RNA-seq)?
To obtain the transcriptional status of biological samples at a specific time
What types of reads are obtained from RNA-Seq?
Exonic Reads, Exon–Intron Junction Reads, Poly(A) Reads
What is the read length range for RNA-Seq reads?
25–450 bp
Which types of RNA are mainly studied using RNA-seq?
mRNA, Small RNA, Noncoding RNA (ncRNA), MicroRNAs
What is the purpose of transcriptome sequencing?
To rapidly and comprehensively obtain the transcriptional status of biological samples at a specific time
What is one of the crucial factors in RNAseq?
The number of sequenced reads for a given sample
What is recommended regarding outliers with over 30% disagreement in raw reads?
They should be discarded
What does the optimal library size depend on in RNAseq?
The complexity of the targeted transcriptome
Which type of reads are preferable to characterize poorly annotated transcriptomes in RNA-seq?
Longer reads
What is one of the quality-control checkpoints for raw reads in RNA-seq?
The overrepresented k-mers
What varies with effect size, sequencing depth, and number of replicates in RNA-seq?
Statistical power to detect differential expression
Which step involves obtaining raw reads, read alignment, and quantification in RNA-seq data acquisition?
Quality control
What is crucial for proper sequencing and subsequent analysis in RNA-seq?
The optimal library size
What should be homogeneous for samples in the same experiments in RNA-seq?
The overrepresented k-mers
Which one of these is true about RNA-seq?
Longer reads are not preferable for characterizing poorly annotated transcriptomes.
Test your understanding of RNA-seq data analysis with this quiz focused on the transcriptome and gene expression. This quiz covers topics discussed in Lecture 7 of BMD 427 Applied Bioinformatics by Marwa Amer, PhD.
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