Gel Electrophoresis Quiz

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Questions and Answers

What is the main principle behind gel electrophoresis?

  • Separation of biomacromolecules based on density and volume
  • Separation of biomacromolecules based on size and charge (correct)
  • Separation of biomacromolecules based on weight and texture
  • Separation of biomacromolecules based on color and shape

How are nucleic acid molecules separated in gel electrophoresis?

  • By using chemical solvents
  • By heating the gel matrix
  • By applying an electric field (correct)
  • By adding magnetic particles

What is the phenomenon called when shorter molecules move faster and migrate farther than longer ones in gel electrophoresis?

  • Sieving (correct)
  • Precipitation
  • Condensation
  • Diffusion

In what field is gel electrophoresis used to separate proteins by charge or size?

<p>Clinical chemistry (C)</p> Signup and view all the answers

What determines the separation of nanoparticles in gel electrophoresis?

<p>The electric field applied (B)</p> Signup and view all the answers

What is the main principle behind gel electrophoresis?

<p>Separation of biomacromolecules based on size and charge (C)</p> Signup and view all the answers

How are nucleic acid molecules separated in gel electrophoresis?

<p>By applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances (B)</p> Signup and view all the answers

What determines the separation of nanoparticles in gel electrophoresis?

<p>The electric field applied to the gel (C)</p> Signup and view all the answers

What is the phenomenon called when shorter molecules move faster and migrate farther than longer ones in gel electrophoresis?

<p>Sieving (B)</p> Signup and view all the answers

How are proteins separated in gel electrophoresis?

<p>By the charge in agarose (D)</p> Signup and view all the answers

What is gel electrophoresis used to separate in clinical chemistry?

<p>Proteins by charge or size (D)</p> Signup and view all the answers

How are nucleic acid molecules separated in gel electrophoresis?

<p>By applying an electric field (D)</p> Signup and view all the answers

Why do shorter nucleic acid molecules move faster and migrate farther than longer ones in gel electrophoresis?

<p>Shorter molecules migrate more easily through the pores of the gel (D)</p> Signup and view all the answers

How are proteins separated in gel electrophoresis?

<p>By the charge in agarose (D)</p> Signup and view all the answers

Apart from biomacromolecules, what else can gel electrophoresis be used to separate?

<p>Nanoparticles (A)</p> Signup and view all the answers

Flashcards

Gel Electrophoresis Principle?

Separation of biomacromolecules based on their size and charge.

Nucleic Acid Separation?

By applying an electric field to move them through a gel matrix.

Gel Sieving?

Shorter molecules move faster and migrate farther than longer ones.

Clinical Chemistry Use?

Proteins by charge or size.

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Nanoparticle separation?

The electric field applied dictates nanoparticle movement.

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Why do shorter molecules move faster?

Shorter molecules migrate more easily through the pores of the gel.

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Nanoparticle separation?

The electric field applied.

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