Gas Chromatography Basics

What is the mobile phase in gas chromatography?

Which type of gas chromatography is most useful for the separation and analysis of gases like CH4 and CO2?

What is the stationary phase in gas liquid chromatography (GLC)?

What is the affinity of solutes in gas solid chromatography (GSC)?

How does gas chromatography achieve separation of components in a sample?

What is the mobile phase in liquid solid chromatography (LSC)?

What type of chromatography involves a stationary phase chemically and permanently held to a porous solid?

In which type of chromatography is the stationary phase a liquid held on a solid support as a thin coat?

What is the mobile phase in gas chromatography (G.C)?

Which type of chromatography involves a mobile phase that is not completely polar or nonpolar?

Gas Chromatography Overview

The mobile phase in gas chromatography is an inert gas, commonly helium or nitrogen, which helps transport the sample through the system.
Gas chromatography is especially effective for separating and analyzing gases like methane (CH4) and carbon dioxide (CO2).
The stationary phase in gas-liquid chromatography (GLC) consists of a liquid coated on an inert solid support, allowing interaction with the solutes.

Separation Mechanisms

In gas-solid chromatography (GSC), solutes exhibit different affinities for the stationary phase, primarily due to adsorption; a stronger affinity leads to slower movement through the column.
Gas chromatography separates components in a sample through a combination of partitioning and vaporization, with differing interactions in the stationary phase causing separation of compounds.

Liquid Chromatography Facts

The mobile phase in liquid-solid chromatography (LSC) moves through a stationary phase composed of a solid, which may interact with solutes differently based on their affinity.
In a specific type of chromatography, the stationary phase is chemically bonded and permanently fixed to a porous solid; this enhances stability and consistency during analysis.
In liquid chromatography, particularly in GLC, the stationary phase is a liquid that remains on a solid support as a thin film, optimizing separation efficacy.

Additional Chromatography Characteristics

A unique type of chromatography utilizes a mobile phase that is moderately polar, neither fully polar nor nonpolar, which can help in separating a broader range of analytes based on their polarity properties.

Test your knowledge about the fundamental principles of gas chromatography, a technique used for the separation of volatile substances in a gaseous mixture. Learn about the injection process, stationary and mobile phases, and the separation of sample components.