intracellular cell signaling
88 Questions
62 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which molecule is responsible for bringing PDK1 and Akt together at the membrane?

  • GC
  • NOs
  • PI3K
  • PIP3 (correct)
  • What is the key mediator that relaxes smooth muscle in blood vessels and visceral organs?

  • NO (correct)
  • PI3K
  • Akt
  • GC
  • What is the enzyme responsible for producing NO from L-arginine?

  • Akt
  • NOs (correct)
  • GC
  • PI3K
  • What molecule is produced from GTP by guanylyl cyclase (GC)?

    <p>cGMP</p> Signup and view all the answers

    What molecule activates a protein kinase (usually PKG) when elevated?

    <p>cGMP</p> Signup and view all the answers

    In smooth muscle, what does the disengagement of myosin from actin lead to?

    <p>Smooth muscle relaxation</p> Signup and view all the answers

    Which G-protein mechanism is unique in that it uses Ca+2 and IP3 and DAG as second messenger systems?

    <p>Gq</p> Signup and view all the answers

    What effect does IP3 have when it is released into the cytosol?

    <p>It causes release of Ca+2 from the endoplasmic reticulum (ER)</p> Signup and view all the answers

    What is the impact of Ca+2 binding to calmodulin?

    <p>It activates calmodulin kinases</p> Signup and view all the answers

    Which G-protein mechanism activates phospholipase C?

    <p>Gq</p> Signup and view all the answers

    What is the impact of Gi-α on adenylyl cyclase activity?

    <p>It inhibits adenylyl cyclase</p> Signup and view all the answers

    What is the impact of Gi-βγ on the membrane potential?

    <p>It stimulates K+ channels</p> Signup and view all the answers

    Which of the following is NOT a function of cell membrane receptors in intracellular signaling?

    <p>Regulating ionic 2nd messengers</p> Signup and view all the answers

    Which of the following best describes transduction in intracellular signaling?

    <p>The process of converting an extracellular signal into an intracellular signal</p> Signup and view all the answers

    What happens when the concentration of the first messenger (ligand) increases?

    <p>The ligand binds to the cell membrane receptor</p> Signup and view all the answers

    What is the role of the activated cell membrane receptor?

    <p>To bind to and activate another protein</p> Signup and view all the answers

    What happens when the second messenger binds to and activates another protein?

    <p>The activated protein will bind to and activate or inactivate downstream effectors</p> Signup and view all the answers

    What happens to the second messenger over time?

    <p>It is metabolized or removed</p> Signup and view all the answers

    Which of the following is true about G-protein-coupled receptors (GPCRs)?

    <p>GPCRs have three protein subunits: alpha, beta, and gamma.</p> Signup and view all the answers

    What happens when a ligand binds to a receptor associated with a Gs GPCR?

    <p>Gs releases GDP and binds GTP at the alpha subunit.</p> Signup and view all the answers

    What is the role of adenylyl cyclase in G-protein-coupled receptor signaling?

    <p>Adenylyl cyclase converts ATP to cAMP.</p> Signup and view all the answers

    What is the role of protein kinase A (PKA) in G-protein-coupled receptor signaling?

    <p>PKA phosphorylates effector proteins.</p> Signup and view all the answers

    What is the enzyme responsible for converting cAMP to 5'-AMP in G-protein-coupled receptor signaling?

    <p>Cyclic AMP phosphodiesterase</p> Signup and view all the answers

    What happens when GTP is hydrolyzed to GDP in G-protein-coupled receptor signaling?

    <p>The G-protein becomes inactivated</p> Signup and view all the answers

    According to the text, what happens to the cytosolic calcium concentration when a channel that allows calcium to cross the cell membrane is opened?

    <p>It increases</p> Signup and view all the answers

    What is the membrane potential change called when a channel allows sodium to enter the cell?

    <p>Depolarization</p> Signup and view all the answers

    Which ligand is an example of a receptor tyrosine kinase?

    <p>All of the above</p> Signup and view all the answers

    What is the key pathway associated with receptor tyrosine kinase activation?

    <p>All of the above</p> Signup and view all the answers

    What is the second messenger in the PI-3-Kinase-Akt pathway?

    <p>PIP3</p> Signup and view all the answers

    Activation of a G-protein-coupled receptor leads to the activation of a protein that binds to a __________ nucleotide

    <p>guanine</p> Signup and view all the answers

    The G-protein-coupled receptor (GPCR) family is the _______ family of cell membrane receptors

    <p>largest</p> Signup and view all the answers

    The activated G-protein modifies the activity of an ________

    <p>enzyme</p> Signup and view all the answers

    The G-protein consists of three subunits: _______, ______, and ______

    <p>α, β, γ</p> Signup and view all the answers

    In the Gs GPCR pathway, Gs binds to and activates ____________

    <p>adenylyl cyclase</p> Signup and view all the answers

    The enzyme responsible for converting cAMP to 5'-AMP is _________

    <p>cyclic AMP phosphodiesterase</p> Signup and view all the answers

    PIP3 is responsible for activating ______

    <p>PDK1</p> Signup and view all the answers

    PDK1 is responsible for activating ______

    <p>Akt</p> Signup and view all the answers

    The production of cGMP is catalyzed by ______

    <p>guanylyl cyclase (GC)</p> Signup and view all the answers

    Elevations in cytosolic cGMP activates a protein kinase called ______

    <p>PKG</p> Signup and view all the answers

    Elevations in cytosolic calcium can activate ______

    <p>nitric oxide synthase (NOs)</p> Signup and view all the answers

    NO binds to and activates ______

    <p>guanylyl cyclase (GC)</p> Signup and view all the answers

    Gi-α inactivates ______

    <p>adenylyl cyclase</p> Signup and view all the answers

    Opening of K+ channels brings the cell closer to its ______ potential for K+

    <p>-90 mV</p> Signup and view all the answers

    The cell membrane is inside-negative with a low cytosolic concentration of sodium and a high concentration of K+

    <p>Nernst</p> Signup and view all the answers

    IP3 is a 2nd messenger that causes release of ______ from where it’s stored in the endoplasmic reticulum (ER)

    <p>Ca+2</p> Signup and view all the answers

    Ca+2 can bind to and activate a number of ______

    <p>proteins</p> Signup and view all the answers

    Opening a channel that allows calcium to cross the cell membrane would increase the cytosolic ______ concentration

    <p>calcium</p> Signup and view all the answers

    Depolarization is the term used when the membrane becomes more ______

    <p>inside-positive</p> Signup and view all the answers

    Hyperpolarization is the term used when the membrane becomes more ______

    <p>inside-negative</p> Signup and view all the answers

    The activation of certain membrane-associated proteins is impacted by making the membrane more ______ or more ______

    Signup and view all the answers

    Opening a channel that allows calcium to cross the cell membrane would increase the cytosolic ______ concentration

    <p>calcium</p> Signup and view all the answers

    Hyperpolarization is the term used when the membrane becomes more ______

    <p>inside-negative</p> Signup and view all the answers

    What is the membrane potential change called when a channel allows sodium to enter the cell?

    <p>depolarization</p> Signup and view all the answers

    The activation of certain membrane-associated proteins is impacted by making the membrane more ______ or more ______

    <p>inside-positive, inside-negative</p> Signup and view all the answers

    Elevations in cytosolic calcium can activate ______

    <p>calmodulin</p> Signup and view all the answers

    Receptors in the cell membrane are key in detecting ______ signals and modifying cell function based on those signals

    <p>extracellular</p> Signup and view all the answers

    Transduction is the intracellular events that transform the ______ signal into an intracellular signal

    <p>extracellular</p> Signup and view all the answers

    Additional signaling events involving the membrane: source of some ______ messengers

    <p>2nd</p> Signup and view all the answers

    Additional signaling events involving the membrane: site where some 2nd messengers ______

    <p>accumulate</p> Signup and view all the answers

    Additional signaling events involving the membrane: site where ionic 2nd messengers are ______

    <p>regulated</p> Signup and view all the answers

    Additional signaling events involving the membrane: important site where regulatory proteins and enzymes ______ and integrate signaling

    <p>localize</p> Signup and view all the answers

    Receptors in the cell membrane are key in detecting ______ signals and modifying cell function based on those signals

    <p>extracellular</p> Signup and view all the answers

    That protein activated a mechanism (in this case an enzyme) that increased the intracellular concentration of the active form of a second ______

    <p>messenger</p> Signup and view all the answers

    The second messenger binds to and activates another ______… and that protein will activate or inactivate other biochemical signaling cascades that have some sort of effect

    <p>protein</p> Signup and view all the answers

    Inactive system: extracellular signal ______

    <p>ends</p> Signup and view all the answers

    As time goes on: ligand releases from receptor receptor-associated effectors ______ 2nd messenger is either metabolized or removed 2nd messenger “inactivator”

    <p>inactivate</p> Signup and view all the answers

    Activation of a G-protein-coupled receptor leads to the activation of a protein that binds to a ______ nucleotide

    <p>GTP</p> Signup and view all the answers

    The G-protein consists of three subunits: ______, ______, and ______

    <p>alpha, beta, gamma</p> Signup and view all the answers

    Opening a channel that allows calcium to cross the cell membrane would increase the cytosolic ______ concentration

    <p>calcium</p> Signup and view all the answers

    Receptors in the cell membrane are key in detecting ______ signals and modifying cell function based on those signals

    <p>extracellular</p> Signup and view all the answers

    What is the impact of Gi-α on adenylyl cyclase activity?

    <p>inhibits</p> Signup and view all the answers

    What is the membrane potential change called when a channel allows sodium to enter the cell?

    <p>depolarization</p> Signup and view all the answers

    Gs releases GDP and binds GTP at the alpha subunit ▪ The βγ subunit detaches from the G-protein 3. Gs binds to and activates adenylyl cyclase ▪ Membrane-bound enzyme that converts ATP to ______

    <p>cAMP</p> Signup and view all the answers

    PK A phosphorylates a multitude of ______ proteins

    <p>effector</p> Signup and view all the answers

    Inactivation of cAMP results when it is converted to 5’-AMP ▪ Enzyme – cyclic AMP ______

    <p>phosphodiesterase</p> Signup and view all the answers

    A ligand binds to a receptor associated with a ______ Gprotein

    <p>Gs</p> Signup and view all the answers

    Opening a channel that allows calcium to cross the cell membrane would increase the cytosolic ______ concentration

    <p>calcium</p> Signup and view all the answers

    NO is a _______ messenger – one of the only ones that can diffuse across the cell membrane and impact other cells

    <p>second</p> Signup and view all the answers

    ______ is responsible for activating guanylyl cyclase (GC)

    <p>NOs</p> Signup and view all the answers

    Elevations in cytosolic ______ activates a protein kinase (usually PKG)

    <p>cGMP</p> Signup and view all the answers

    In smooth muscle, the disengagement of myosin from actin leads to _______ muscle relaxation

    <p>smooth</p> Signup and view all the answers

    ______ brings PDK1 and Akt together at the membrane

    <p>PIP3</p> Signup and view all the answers

    NO is produced enzymatically by the action of nitric oxide synthase (NOs) on _______

    <p>L-arginine</p> Signup and view all the answers

    Opening a channel that allows calcium to cross the cell membrane would increase the cytosolic ______ concentration

    <p>calcium</p> Signup and view all the answers

    Depolarization is the term used when the membrane becomes more ______

    <p>inside-positive</p> Signup and view all the answers

    Hyperpolarization is the term used when the membrane becomes more ______

    <p>inside-negative</p> Signup and view all the answers

    The activation of certain membrane-associated proteins is impacted by making the membrane more ______ or more ______

    <p>inside-positive, inside-negative</p> Signup and view all the answers

    The Ras-Raf-MAP kinase pathway is the pathway most commonly associated with ______ activation

    <p>RTK</p> Signup and view all the answers

    Study Notes

    Protein Interactions and Mediators

    • PDK1 and Akt are brought together at the membrane by the molecule PIP3.
    • The key mediator that relaxes smooth muscle in blood vessels and visceral organs is Nitric Oxide (NO).
    • Nitric oxide synthase (NOS) is the enzyme that produces NO from L-arginine.
    • Guanylyl cyclase (GC) produces cGMP from GTP.
    • cGMP activates a protein kinase, usually PKG, when elevated.

    Smooth Muscle Dynamics

    • Disengagement of myosin from actin in smooth muscle leads to muscle relaxation.
    • The G-protein mechanism that involves Ca²⁺ relies on IP3 and DAG as second messengers.
    • IP3 triggers the release of Ca²⁺ from the endoplasmic reticulum (ER) when released into the cytosol.
    • Ca²⁺ binding to calmodulin activates various target proteins.

    G-Protein Coupling and Effects

    • The G-protein mechanism activating phospholipase C is associated with certain types of GPCRs.
    • Gi-α inhibits adenylyl cyclase activity, leading to reduced cAMP production.
    • Gi-βγ can hyperpolarize the membrane by affecting the membrane potential.

    Cell Membrane Receptors and Signaling

    • Functions of cell membrane receptors in intracellular signaling include detecting signals but do not typically encompass channel characteristics.
    • Transduction refers to the process that converts an extracellular signal into an intracellular signal.
    • An increase in the first messenger's concentration amplifies intracellular responses.
    • Activated cell membrane receptors initiate downstream signaling cascades.
    • Second messengers amplify the signaling effect when they bind to target proteins.

    G-Protein-Coupled Receptors (GPCRs)

    • GPCR family is the largest family of cell membrane receptors.
    • When a ligand binds to a Gs GPCR, it activates adenylyl cyclase.
    • Adenylyl cyclase converts ATP to cAMP, leading to various signaling effects.
    • PKA (protein kinase A) phosphorylates target proteins, affecting multiple cellular processes.

    Second Messengers and Pathways

    • Phosphodiesterase is responsible for converting cAMP to 5'-AMP, leading to its inactivation.
    • GTP hydrolysis to GDP leads to the inactivation of the G-protein in GPCR signaling.
    • The change in cytosolic calcium concentration occurs when channels open, facilitating calcium entry.
    • Depolarization occurs when sodium enters the cell, while hyperpolarization occurs when the cell becomes more negative.

    Key Signaling Components

    • Receptor tyrosine kinases are crucial for signaling pathways like the Ras-Raf-MAP kinase pathway.
    • PIP3 activates PDK1, which subsequently activates Akt.
    • The production of cGMP is catalyzed by activation of guanylyl cyclase, often by NO.
    • Activated PKA regulates various other signaling proteins, further propagating the signal.

    Membrane Potential and Associated Effects

    • Opening K⁺ channels can bring the cell closer to its equilibrium potential.
    • Receptors enable the detection of extracellular signals and their integration into cellular functions.
    • Membrane-associated proteins are influenced by changes in membrane potential, affecting their activity.

    Signal Termination and Regulation

    • With time, ligands detach from receptors, leading to a decrease in signaling as second messengers are metabolized or removed.
    • Active G-protein components include three subunits: α, β, and γ.
    • Ca²⁺ as a second messenger can activate additional signaling pathways, emphasizing its role in various cellular processes.

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Description

    Quiz: Understanding G-Protein Mechanisms - Gi and Gq Test your knowledge on the different G-protein mechanisms with this quiz. Discover the roles of Gi and Gq in modulating cellular responses and learn how they activate second messengers to regulate effector functions in various cell types. Challenge yourself and deepen your understanding of these important signaling pathways.

    More Like This

    Embryology Quiz 1
    36 questions

    Embryology Quiz 1

    AdmirableMotif avatar
    AdmirableMotif
    Cell Signaling Flashcards
    41 questions

    Cell Signaling Flashcards

    SpellboundEllipsis avatar
    SpellboundEllipsis
    Cell Signaling and Receptor Molecules
    19 questions
    Biology on Arachidonic Acid Functions
    44 questions
    Use Quizgecko on...
    Browser
    Browser