Podcast
Questions and Answers
What is the purpose of leaving a gap between the lyophilizing machine and stopper?
What is the purpose of leaving a gap between the lyophilizing machine and stopper?
- To accelerate the evaporation of water
- To prevent the formation of ice during freezing
- To increase the pressure inside the machine
- To accommodate water molecules during lyophilization (correct)
Why is it necessary to lower the temperature and pressure to create a vacuum during lyophilization?
Why is it necessary to lower the temperature and pressure to create a vacuum during lyophilization?
- To prevent the formation of ice crystals
- To maintain the stability of the protein
- To create ice without forming a liquid first (correct)
- To accelerate the evaporation of water
What happens to the pH when using a phosphate buffer at lower temperatures during lyophilization?
What happens to the pH when using a phosphate buffer at lower temperatures during lyophilization?
- The pH increases due to higher solubility of phosphate salt at lower temperatures
- The pH decreases due to lower solubility of phosphate salt at lower temperatures (correct)
- The pH remains constant regardless of temperature
- The pH becomes acidic due to the formation of ice crystals
Why is it advised not to use a phosphate buffer for unstable molecules during lyophilization?
Why is it advised not to use a phosphate buffer for unstable molecules during lyophilization?
What is the likely stress encountered by protein molecules during freezing and drying that could cause degradation?
What is the likely stress encountered by protein molecules during freezing and drying that could cause degradation?
What is the likely outcome to the residual water content in the powder using a drying gas with high temperature and low humidity at a high aspirator flow?
What is the likely outcome to the residual water content in the powder using a drying gas with high temperature and low humidity at a high aspirator flow?
What method can be used to detect aggregation in proteins?
What method can be used to detect aggregation in proteins?
Which spectroscopic method provides insights into aromatic residues, disulfide bonds, and tertiary structures?
Which spectroscopic method provides insights into aromatic residues, disulfide bonds, and tertiary structures?
What does ionic exchange HPLC separate proteins based on?
What does ionic exchange HPLC separate proteins based on?
What does Size Exclusion HPLC detect in proteins?
What does Size Exclusion HPLC detect in proteins?
What is the primary purpose of spray freeze drying?
What is the primary purpose of spray freeze drying?
What is the likely effect of phosphate buffers on pH at lower temperatures?
What is the likely effect of phosphate buffers on pH at lower temperatures?
What is the likely impact of phosphate buffers on stability for unstable molecules?
What is the likely impact of phosphate buffers on stability for unstable molecules?
What is the primary function of a phosphate buffer?
What is the primary function of a phosphate buffer?
What is the purpose of spray drying gas with high temperature and low humidity?
What is the purpose of spray drying gas with high temperature and low humidity?
What is the likely outcome of increasing aspirator air flow in the spray drying process?
What is the likely outcome of increasing aspirator air flow in the spray drying process?
