Formation of Germ Layers and Fertilization

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Questions and Answers

What is a result of fertilization in terms of chromosome number?

  • The zygote has a triploid chromosome number.
  • The diploid chromosome number is reduced to 23.
  • The germ layers are formed without any change in chromosome number.
  • The diploid chromosome number is restored to 46. (correct)

What determines the sex of the offspring at fertilization?

  • The type of spermatozoon that fertilizes the ovum. (correct)
  • The number of chromosomes in the spermatozoon.
  • The history of the parents' genetic traits.
  • The age of the parents at the time of fertilization.

How many chromosomes are present in a zygote if it is fertilized by a Y-bearing spermatozoon?

  • 46 + X + Y
  • 44 + Y
  • 44 + X + Y (correct)
  • 22 + Y chromosomes

During DNA replication, what structure is formed?

<p>Two identical chromatids for each chromosome. (D)</p> Signup and view all the answers

Which statement about chromosome behavior during cell division is correct?

<p>One chromosome from each pair moves to opposite ends of the spindle. (D)</p> Signup and view all the answers

What is the chromosomal composition of a girl offspring?

<p>44 + X + X chromosomes. (A)</p> Signup and view all the answers

What is the origin of the chromosomes in each pair?

<p>One chromosome derives from the mother and the other from the father. (C)</p> Signup and view all the answers

What process begins after fertilization in the ovum?

<p>Cleavage. (A)</p> Signup and view all the answers

What is the term for the fertilized ovum when it consists of 16 cells?

<p>Morula (B)</p> Signup and view all the answers

Which two germ layers are formed first in the embryonic disc?

<p>Ectoderm and endoderm (A)</p> Signup and view all the answers

What structure forms the membrane called the chorion?

<p>Somatopleuric mesoderm and trophoblast (D)</p> Signup and view all the answers

What separates the inner cell mass from the trophoblast in the morula stage?

<p>Fluid (A)</p> Signup and view all the answers

Which cavity appears first on the ectodermal side of the embryonic disc?

<p>Amniotic cavity (B)</p> Signup and view all the answers

What is the name of the area in the embryonic disc that is not separated by mesoderm?

<p>Prochordal plate (A)</p> Signup and view all the answers

What occurs to the morula as fluid increases from the uterine cavity?

<p>It takes the shape of a cyst. (A)</p> Signup and view all the answers

What do the cells of the primitive streak contribute to in the embryonic development?

<p>Development of the mesoderm (D)</p> Signup and view all the answers

At what stage is the embryo typically placed inside the uterus?

<p>8-cell stage (D)</p> Signup and view all the answers

At what stage does the morula develop into a blastocyst?

<p>After cleavage (C)</p> Signup and view all the answers

What are the two types of spermatozoa in terms of their chromosome composition?

<p>22 + X and 22 + Y (D)</p> Signup and view all the answers

Which pole of the blastocyst is defined by the attachment of the inner cell mass?

<p>Embryonic pole (B)</p> Signup and view all the answers

What is the cavity inside the blastocyst called?

<p>Blastocoele (C)</p> Signup and view all the answers

Which layer of cells becomes flattened as the morula develops into a blastocyst?

<p>Trophoblast (A)</p> Signup and view all the answers

What prevents the embryo from adhering to the uterine epithelium during its travel?

<p>Zona pellucida (D)</p> Signup and view all the answers

What percentage of trials result in successful implantation of the embryo?

<p>20% (A)</p> Signup and view all the answers

What is the term used to describe the process of the ovum dividing into smaller cells?

<p>Cleavage (D)</p> Signup and view all the answers

What stage follows the '3-cell' stage in the cleavage process?

<p>4-cell stage (A)</p> Signup and view all the answers

What is the structure that the morula resembles due to its appearance?

<p>Mulberry (C)</p> Signup and view all the answers

What is the outer layer of cells surrounding the inner cell mass in the morula called?

<p>Trophoblast (B)</p> Signup and view all the answers

What is one of the primary hormones administered to women to stimulate follicle growth before ovulation?

<p>Gonadotropins (A)</p> Signup and view all the answers

What is the name of the cluster of cells formed after 16 divisions of the ovum?

<p>Morula (D)</p> Signup and view all the answers

In vitro fertilization involves which of the following processes?

<p>Extraction of the ovum and fertilization outside the body (B)</p> Signup and view all the answers

What does the inner cell mass of the morula develop into?

<p>Embryo (B)</p> Signup and view all the answers

What is the role of growth factors in the context of stem cell culture?

<p>They help maintain stem cells in an undifferentiated state. (A)</p> Signup and view all the answers

Which type of mesoderm is formed as a result of cells from the epiblast migrating during development?

<p>Extra-embryonic mesoderm (D)</p> Signup and view all the answers

What is the advantage of using stem cells that are genetically identical to the patient?

<p>Elimination of immune response against transplanted tissues. (B)</p> Signup and view all the answers

Which of the following cells can stem cells differentiate into?

<p>Various types of adult cells, e.g., neurons and blood cells. (C)</p> Signup and view all the answers

Where can stem cells besides embryonic sources be isolated from?

<p>Adult tissues such as bone marrow and brain. (A)</p> Signup and view all the answers

What is the main purpose of therapeutic stem cell cloning?

<p>To produce genetically matched tissues for patients. (A)</p> Signup and view all the answers

What is the consequence of introducing a patient's cell nucleus into an embryonic stem cell?

<p>It allows the stem cell to produce tissues compatible with the patient. (C)</p> Signup and view all the answers

How do embryonic stem cells maintain their undifferentiated state during in vitro culture?

<p>Through exposure to specific growth factors. (C)</p> Signup and view all the answers

What is a significant drawback of using adult stem cells in research?

<p>They are difficult to culture in laboratories. (B)</p> Signup and view all the answers

What ethical concern is raised regarding stem cell research involving human embryos?

<p>It is seen as a violation of natural laws. (D)</p> Signup and view all the answers

What event marks the beginning of gastrulation in embryonic development?

<p>Establishment of the primitive streak. (A)</p> Signup and view all the answers

At which stage of pregnancy is the embryo referred to as a morula?

<p>At day 4. (A)</p> Signup and view all the answers

Which teratogenic influence during the pre-organogenesis period is likely to lead to the embryo's death?

<p>Environmental toxins. (A)</p> Signup and view all the answers

During which week of pregnancy does the bilaminar disc form?

<p>2nd week. (C)</p> Signup and view all the answers

What is the significance of the prochordal plate and primitive streak being seen at day 14?

<p>Marks the onset of gastrulation. (C)</p> Signup and view all the answers

What are congenital anomalies most often attributed to during the embryonic period?

<p>Teratogens. (D)</p> Signup and view all the answers

Flashcards

Fertilization location

Fertilization of an ovum occurs in the ampulla of the uterine tube.

Morula formation

The fertilized ovum undergoes cell divisions (cleavage), and when it has 16 cells, it's a morula.

Blastocyst formation

A morula develops into a blastocyst, containing an inner cell mass and trophoblast.

Germ layers

An embryonic disc with ectoderm, endoderm, and mesoderm arises from the inner cell mass, forming the three germ layers.

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Amniotic cavity

A cavity develops on the ectodermal side of the embryonic disc, forming the amniotic cavity.

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Yolk sac

A cavity forms on the endodermal side of the disc, called the yolk sac.

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Chorion formation

The trophoblast and underlying mesoderm create the chorion membrane.

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Mesoderm formation

Cells from the primitive streak differentiate into mesoderm, the middle germ layer between the ectoderm and endoderm.

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Chromosome Replication

DNA replication creates a second chromatid, making each chromosome double-stranded, ready for division.

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Diploid Chromosome Number

The full set of chromosomes, restored to 46 after fertilization of egg and sperm.

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Sex Determination (Fertilization)

The sex of a child is decided at fertilization. X-bearing sperm leads to a girl; Y-bearing, to a boy.

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Chromosome Pairs (In Humans)

Human cells have 23 pairs of chromosomes. One chromosome from each pair comes from each parent.

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Mitosis

The process where each chromosome splits into two, each containing identical genetic material

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Spindle Formation

The spindle is a structure that forms in the cell during division to help move chromosomes.

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Cleavage

The fertilized egg dividing into multiple cells. This is a key step after fertilization.

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X-bearing Sperm

Sperm containing an X chromosome resulting in female offspring.

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Daughter cells

Cells produced after a parent cell divides.

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Zona pellucida

A protective layer surrounding the oocyte or the embryo in the early stages of development.

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Morula

A solid ball of cells formed during early embryonic development, resembling a mulberry.

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In vitro fertilization

Fertilization of an egg outside the body.

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Embryoblast

The inner cell mass of the blastocyst, which will develop into the embryo.

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Trophoblast

The outer layer of cells in a blastocyst that will form the placenta and other extra-embryonic tissues.

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Test-tube baby

A baby conceived using in vitro fertilization (IVF).

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Embryo Development Medium

The environment where fertilization and early embryo development occur.

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8-cell Stage

Embryo placed inside the uterus after reaching this eight-celled stage during development.

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Implantation Success Rate

Only about 20% of embryo transfers result in successful implantation.

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Ovum Chromosome Content

Each egg cell (ovum) contains 22 chromosomes plus an X chromosome.

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Spermatozoa Types

Sperm cells are categorized into two types based on their chromosome content (22+X or 22+Y).

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Blastocyst Poles

The blastocyst has an embryonic (animal) pole where the inner cell mass attaches, and an abembryonic pole.

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Zona Pellucida Role

The zona pellucida prevents the embryo from sticking to uterine tissues while it travels to the uterus.

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Primitive Streak

A thickened band of cells that forms on the dorsal surface of the embryonic disc during gastrulation. It is crucial for establishing the body axis and initiating development of the mesoderm.

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Intra-embryonic Mesoderm

The middle germ layer that arises from the primitive streak during gastrulation. It will give rise to various tissues and organs in the developing embryo, including muscle, bone, and blood.

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Extra-embryonic Mesoderm

Mesoderm that arises outside the embryo, specifically from the connecting stalk. It contributes to the development of structures like the amnion and chorion, supporting the growing embryo.

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Connecting Stalk

A structure attaching the embryo to the placenta. It contains blood vessels and serves as a conduit for nutrient exchange and waste removal.

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Extra-embryonic Coelom

The cavity that forms within the extra-embryonic mesoderm, surrounding the amnion and yolk sac. It plays a role in separation and protection of these structures.

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Therapeutic Stem Cell Cloning

A technique where the nucleus of a patient's cell is introduced into an embryonic stem cell, creating cells genetically identical to the patient. These cells can then differentiate into various cell types for treating diseases.

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Stem Cell Differentiation

The process where stem cells, with potential to become various cell types, develop into specific types of cells like neurons, muscle cells, or blood cells, depending on the environmental factors.

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Adult Stem Cells

Stem cells found in some adult tissues like bone marrow, brain, and skeletal muscle, with limited potential to differentiate into different cell types, compared to embryonic stem cells.

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Pre-organogenesis period

The first two weeks of pregnancy where the embryo develops but no organs are formed.

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Bilaminar disc

The embryo starts as a flat, two-layered structure with an outer ectoderm layer and an inner endoderm layer.

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Gastrulation

The process where the bilaminar disc transforms into a three-layered structure called the trilaminar disc.

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Trilaminar disc

The three-layered embryo structure formed during gastrulation, consisting of ectoderm, mesoderm, and endoderm.

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Ectoderm

The outer layer of the trilaminar disc that forms the skin, hair, and nervous system.

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Mesoderm

The middle layer of the trilaminar disc that forms muscles, bones, and the circulatory system.

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Endoderm

The inner layer of the trilaminar disc that forms the lining of the digestive tract and lungs.

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Congenital anomalies

Birth defects that occur during embryonic development, often due to teratogens.

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Study Notes

Formation of Germ Layers

  • Fertilization occurs in the ampulla of the uterine tube
  • Fertilized ovum divides (cleavage)
  • 16 cells form morula
  • Morula develops into blastocyst with inner cell mass and trophoblast
  • Inner cell mass multiplies and forms an embryonic disc
  • Embryonic disc has ectoderm and endoderm
  • Mesoderm forms between ectoderm and endoderm
  • Amniotic cavity forms on ectodermal side
  • Yolk sac develops on endodermal side
  • Extra-embryonic mesoderm separates cavities from trophoblast
  • Extra-embryonic coelom splits extra-embryonic mesoderm into somatopleuric and splanchnopleuric layers
  • Chorion develops from trophoblast and somatopleuric mesoderm
  • Amnion forms from cells of amniotic cavity
  • Prochordal plate appears near one edge of embryonic disc
  • Primitive streak forms on embryonic disc
  • Primitive streak cells form mesoderm
  • Cloacal membrane forms caudal to primitive disc
  • This is made of ectoderm and endoderm

Fertilization

  • Primary oocyte matures within ovarian follicle
  • Becomes secondary oocyte during ovulation
  • Secondary oocyte enters fallopian tube
  • Sperm fertilizes oocyte in ampulla
  • Sperm penetrates zona pellucida
  • Nuclei of ovum and sperm fuse (pronuclei)
  • 46 chromosomes restored
  • Sex determined by sperm type (X or Y)
  • Ovum divides into cells (cleavage)

Cleavage

  • Two-celled stage forms
  • 3-celled stage, 4-celled stage, etc
  • Morula Forms
  • Blastocyst forms with blastocoele and inner cell mass
  • Blastocyst implants in uterine wall
  • Zona pellucida disappears
  • Trophoblast (outer cell layer) sticks to uterine wall
  • Additional nutrition available from uterine secretions and yolk

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