12 Questions
What is the most commonly used fixative mentioned in the text?
Formaldehyde 10% neutral buffered formalin
What type of pigment does Mercuric Chloride produce?
Mercury pigment
How can the black acid hematin / formalin pigment be removed?
Alcoholic picric acid
Which fixative is considered a post fixative in the text?
Bouin solution
What is the main purpose of using Phosphotungstic acid-hematoxytin (PTAH) for muscle tissues?
To stain cross-striations
Why can Glutaraldehyde not be used on Schiff techniques like PAS?
It causes a false positive reaction
Which type of microscopy is considered a good screening method for the formalin pigment mentioned in the text?
Polarization microscopy
What is the main purpose of using Bouin's solution as a fixative according to the text?
To stain tissues with trichrome stain
Which of the following fixatives is mentioned in the text as producing a $\text{mercury pigment}$ that is difficult to remove?
Mercuric chloride
Why is Glutaraldehyde mentioned as not being suitable for use with Schiff techniques like PAS?
Glutaraldehyde causes a false positive reaction with the Schiff reagent
What is the main purpose of using Phosphotungstic acid-hematoxylin (PTAH) staining on muscle tissues according to the text?
To preserve the cross-striations in muscle tissue
Which fixative is mentioned in the text as a 'post fixative' that is used after an initial fixation with another agent?
Bouin's solution
Test your knowledge on formaldehyde as a fixative and pigments that may be produced. Learn about polarized microscopy for screening, prevention methods, and removal techniques. Also, identify the staining method for muscle cross-striations.
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