Formaldehyde Fixative and Pigments Quiz

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Questions and Answers

What is the most commonly used fixative mentioned in the text?

  • Formaldehyde 10% neutral buffered formalin (correct)
  • Glutaraldehyde
  • Mercuric Chloride
  • Zenker solution

What type of pigment does Mercuric Chloride produce?

  • Mercury pigment (correct)
  • Yellow pigment
  • Dark brown pigment
  • Black acid hematin / formalin pigment

How can the black acid hematin / formalin pigment be removed?

  • Treat with iodine followed by sodium thiosulfate
  • Washing with 50-70% alcohol
  • Use Orth's Solution
  • Alcoholic picric acid (correct)

Which fixative is considered a post fixative in the text?

<p>Bouin solution (C)</p> Signup and view all the answers

What is the main purpose of using Phosphotungstic acid-hematoxytin (PTAH) for muscle tissues?

<p>To stain cross-striations (B)</p> Signup and view all the answers

Why can Glutaraldehyde not be used on Schiff techniques like PAS?

<p>It causes a false positive reaction (C)</p> Signup and view all the answers

Which type of microscopy is considered a good screening method for the formalin pigment mentioned in the text?

<p>Polarization microscopy (A)</p> Signup and view all the answers

What is the main purpose of using Bouin's solution as a fixative according to the text?

<p>To stain tissues with trichrome stain (B)</p> Signup and view all the answers

Which of the following fixatives is mentioned in the text as producing a $\text{mercury pigment}$ that is difficult to remove?

<p>Mercuric chloride (C)</p> Signup and view all the answers

Why is Glutaraldehyde mentioned as not being suitable for use with Schiff techniques like PAS?

<p>Glutaraldehyde causes a false positive reaction with the Schiff reagent (A)</p> Signup and view all the answers

What is the main purpose of using Phosphotungstic acid-hematoxylin (PTAH) staining on muscle tissues according to the text?

<p>To preserve the cross-striations in muscle tissue (D)</p> Signup and view all the answers

Which fixative is mentioned in the text as a 'post fixative' that is used after an initial fixation with another agent?

<p>Bouin's solution (C)</p> Signup and view all the answers

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