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Formaldehyde Fixative and Pigments Quiz

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Questions and Answers

What is the most commonly used fixative mentioned in the text?

Formaldehyde 10% neutral buffered formalin (correct)

Glutaraldehyde

Mercuric Chloride

Zenker solution

What type of pigment does Mercuric Chloride produce?

Mercury pigment (correct)

Yellow pigment

Dark brown pigment

Black acid hematin / formalin pigment

How can the black acid hematin / formalin pigment be removed?

Treat with iodine followed by sodium thiosulfate

Washing with 50-70% alcohol

Use Orth's Solution

Alcoholic picric acid (correct)

Which fixative is considered a post fixative in the text?

Bouin solution Signup and view all the answers

What is the main purpose of using Phosphotungstic acid-hematoxytin (PTAH) for muscle tissues?

To stain cross-striations Signup and view all the answers

Why can Glutaraldehyde not be used on Schiff techniques like PAS?

It causes a false positive reaction Signup and view all the answers

Which type of microscopy is considered a good screening method for the formalin pigment mentioned in the text?

Polarization microscopy Signup and view all the answers

What is the main purpose of using Bouin's solution as a fixative according to the text?

To stain tissues with trichrome stain Signup and view all the answers

Which of the following fixatives is mentioned in the text as producing a $\text{mercury pigment}$ that is difficult to remove?

Mercuric chloride Signup and view all the answers

Why is Glutaraldehyde mentioned as not being suitable for use with Schiff techniques like PAS?

Glutaraldehyde causes a false positive reaction with the Schiff reagent Signup and view all the answers

What is the main purpose of using Phosphotungstic acid-hematoxylin (PTAH) staining on muscle tissues according to the text?

To preserve the cross-striations in muscle tissue Signup and view all the answers

Which fixative is mentioned in the text as a 'post fixative' that is used after an initial fixation with another agent?

Bouin's solution Signup and view all the answers