Podcast
Questions and Answers
What are the primary aims of Forest Biotechnology?
The main aims of Forest Biotechnology include recovering and maintaining natural resources, improving ecosystem services, and using tree species more efficiently and sustainably.
Which branches of biotechnology can Forest Biotechnology be associated with?
Forest Biotechnology can be associated with Green, White, Grey, and Red Biotechnologies.
How does biotechnology integrate science and engineering?
Biotechnology applies science and engineering to utilize living organisms, or their parts and products in both natural and modified forms.
What distinguishes modern biotechnology from traditional biotechnology?
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What are the criteria for an ideal model plant in tree biotechnology?
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Why are Populus and Salix preferred over Arabidopsis as model plants for tree biotechnology?
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What is a significant drawback of using Arabidopsis as a model for biotechnological research in trees?
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What was a pivotal moment in the history of modern biotechnology?
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What benefits do PGPR provide to in vitro plants?
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Name two other microorganisms, besides rhizobacteria, that are considered Plant Growth Promoting Microorganisms.
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Which tree species was first replicated using somatic embryogenesis and in what decade?
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What characterizes a synthetic seed?
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What role do antioxidants like ascorbic and citric acid play in micropropagation?
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Explain the process involved in haploid technology.
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Why is the development of doubled haploid plants advantageous in tree breeding?
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What are the main limitations of using Arabidopsis as a model tree species?
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List the first step in the somatic embryogenesis protocol.
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How do plant-growth promoting yeasts contribute to plant health?
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Explain why the domestication of tree species is vital for bioeconomy.
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What is the main difference between somatic and zygotic embryos?
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Define what is meant by an 'embling' in the context of somatic embryogenesis.
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What is the role of mycorrhizal fungi in plant growth?
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List the main steps involved in the domestication process of agroforestry trees.
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Describe the difference between direct and indirect micropropagation.
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Why is tree cloning beneficial for conservation projects?
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What are the stages involved in micropropagation by axillary budding?
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Why is frequent subculturing important in the micropropagation process?
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What is the role of auxins and cytokinins during shoot multiplication in micropropagation?
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What are some environmental factors that can help reduce phenolic exudates in explants?
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How can some rhizobacteria (PGPR) benefit in vitro plants?
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Describe adventitious budding in the context of micropropagation.
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What is the significance of selecting a mother donor plant in micropropagation?
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What in vitro protocol can be used to maintain genetic fidelity with respect to the mother plant?
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Describe one method to increase genetic variability in a species using in vitro culture.
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What is somaclonal variation and how can it occur?
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What significance did Taxol have in the medical field upon its discovery?
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What advancements were made in the production of Taxol from plant cell cultures?
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List two biotech products that can be derived from a callus culture.
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What is protoplast fusion, and why is it used in biotechnology?
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How can in vitro selection be utilized to improve stress tolerance in plants?
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What role do growth regulators, particularly auxins, play in the induction of adventitious buds?
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Why is it important to wash and sterilize explants before inducing bud formation?
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What is the purpose of acclimatizing in vitro plants before planting them in soil?
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Identify two factors, besides genotype, that can affect the success of in vitro culture.
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What are the visual characteristics of a vitrified plant, and what condition leads to this phenomenon?
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Describe the phenomenon of phenolic exudation and its potential impact on in vitro cultures.
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Explain the difference between axillary budding and adventitious budding in terms of stability and multiplication rates.
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What adjustments must be made to light and humidity during the acclimatization process?
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Study Notes
Forest Biotechnology Aims
- Aims to achieve sustainable development goals through bioeconomy systems.
- Improves ecosystem services and uses tree parts/products more sustainably.
- Uses technologies to efficiently utilize tree species (cells, tissues, organs or products like primary/secondary metabolites).
Biotechnology Definition & Types
- Biotechnology applies science and engineering to utilize living organisms, their parts, or products.
- Traditional biotechnology has been used for centuries (e.g., food fermentation).
- Modern biotechnology includes newer techniques like genetic engineering and cell fusion.
- Modern biotechnology began around 1973 with recombinant DNA.
Model Plants for Tree Biotechnology
- Arabidopsis thaliana is a good model for herbaceous plants due to small genome size, fast life cycle, transformability, and wide distribution.
- Populus and Salix (trees) are preferable models for biotechnology due to their smaller genomes (450-550 Mbp).
Disadvantages of Arabidopsis for Biotech Trees
- Arabidopsis lacks secondary wood production.
- It isn't a long-lived organism.
- It lacks a dormancy period.
- It has lower crown complexity.
Domestication Importance in Bioeconomy
- Bioeconomy utilizes renewable resources for food, materials, and energy.
- Domestication enhances wood and derivative production.
- It helps mitigate climate change, recover ecosystem services, and reduce pressure on natural forests.
- It allows for genetic, ecological, and pedoclimatic studies on different species.
Domestication Process Steps
- Exploration and collection of species.
- Evaluation of traits.
- Tree breeding.
- Propagation techniques.
- Germoplasm modification.
- Germoplasm distribution.
- Utilization and marketing.
Tree Cloning in Conservation
- Cloning preserves genetic material in both situ and ex situ (outside natural habitat) collections.
- Preserves genetic diversity.
Micropropagation by Axillary Budding
- Selecting a suitable mother plant and explant.
- Sterilizing the explant (e.g., 20-minute wash, 1-2% sodium hypochlorite or 0.1% mercuric chloride, and distilled water rinse)
- Culture initiation and establishment.
- Shoot multiplication (balancing auxins and cytokinins).
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Description
Explore the key concepts of forest biotechnology and its role in achieving sustainable development goals. This quiz covers the definitions, types of biotechnology, and model plants used in tree biotechnology. Learn how these technologies can improve ecosystem services and utilize tree resources more efficiently.