Food Tests: Starch, Glucose, Protein and Lipids

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Questions and Answers

In the Benedict's test, what does a green/yellow color indicate after heating the sample in a water bath?

  • Medium concentration of glucose
  • Absence of glucose
  • Low concentration of glucose (correct)
  • High concentration of glucose

If a food sample remains orange/brown after adding iodine solution, what can you conclude?

  • The sample contains a high concentration of starch.
  • The sample contains a low concentration of starch.
  • The sample contains a moderate amount of glucose.
  • The sample does not contain starch. (correct)

What elements are present in both proteins and lipids?

  • Carbon, Hydrogen, Oxygen (correct)
  • Hydrogen, Oxygen, Sulphur
  • Carbon, Hydrogen, Nitrogen
  • Carbon, Oxygen, Nitrogen

Which of the following is the correct procedure for the emulsion test for lipids?

<p>Add ethanol to the sample, shake, then add water and mix. (B)</p> Signup and view all the answers

What does a purple color indicate in the Biuret's test?

<p>Presence of protein (B)</p> Signup and view all the answers

What is the purpose of using ethanol in the emulsion test for lipids?

<p>To dissolve the lipid molecules. (D)</p> Signup and view all the answers

A food sample is tested using Biuret's solution, and the solution remains blue. What conclusion can be drawn?

<p>The sample does not contain protein. (A)</p> Signup and view all the answers

Which of the following best describes the function of lipids in living organisms?

<p>Providing insulation and protecting organs (B)</p> Signup and view all the answers

Flashcards

Test for Starch

Uses iodine solution; turns blue/black for starch.

Benedict’s Test

Tests for glucose; colors range from blue to brick red.

Protein Structure

Proteins are polymers made from amino acids.

Testing for Protein

Biuret’s test; turns purple if protein is present.

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Lipid Structure

Lipids consist of glycerol and three fatty acids.

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Emulsion Test

Tests for lipids; cloudy white solution indicates lipids.

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Glucose Concentration

Color changes in Benedict’s test indicate glucose levels: green/yellow, orange, brick red.

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Elements in Proteins

Proteins contain carbon, hydrogen, oxygen, nitrogen, and sulfur.

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Study Notes

Food Testing - Starch and Glucose

  • Benedict's test detects simple sugars like glucose

    • Prepare a food sample
    • Add 5ml of food sample to a test tube
    • Add 5ml of Benedict's solution using a syringe and mix
    • Place the test tube in a water bath at 80°C for 10 minutes
    • Record the colour change after 10 minutes
      • Negative result (no glucose) - blue
      • Low glucose concentration - green/yellow
      • Medium glucose concentration - orange
      • High glucose concentration - brick red
  • Iodine test detects starch

    • Prepare the food sample
    • Place a small sample on a spotting tile
    • Add 2-3 drops of iodine solution to the sample
    • Observe the colour change
      • Negative result (no starch) - orange/brown
      • Positive result - blue/black

Food Testing - Protein and Lipids

  • Biuret's test detects protein

    • Prepare 5ml of food sample
    • Add 5ml of Biuret's solution
    • Mix
    • Observe the colour change
      • Negative result - solution remains blue
      • Positive result - solution turns purple
  • Emulsion test detects lipids

    • Place a small spatula of food sample in a test tube
    • Add 5ml of ethanol and shake (dissolves sample)
    • Add 5ml of water and mix
    • Observe the result
      • Positive result - solution turns cloudy white
      • Negative result - solution remains clear

Food Testing - General

  • Food preparation for testing:
    • Prepare the food sample (e.g. bread)
    • Place the sample into a mortar and add a small amount of water
    • Grind the food using a pestle
    • Add 5ml of water and mix
    • Fold a filter paper and place it into a funnel
    • Filter the food sample into a small beaker.

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Protein & Lipid Tests PDF

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