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Questions and Answers
What types of antibiotics is the agent sensitive to?
What types of antibiotics is the agent sensitive to?
The agent is sensitive to streptomycin, tetracycline, chloramphenicol, kanamycin, and novobiocin.
At what temperature and duration can the agent be killed by heat?
At what temperature and duration can the agent be killed by heat?
The agent can be killed by heat at 55˚C for 15 minutes.
What hygienic measures can help prevent infections caused by the agent?
What hygienic measures can help prevent infections caused by the agent?
Properly heated municipal water provision, boiling or chemically treating water, and proper sewage disposal.
Why should seafood not be eaten raw regarding food safety?
Why should seafood not be eaten raw regarding food safety?
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How do foodborne viruses primarily transmit infections?
How do foodborne viruses primarily transmit infections?
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What is the size range of foodborne viruses?
What is the size range of foodborne viruses?
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Where do viruses replicate, and why is it significant?
Where do viruses replicate, and why is it significant?
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What type of contamination leads to infectious hepatitis?
What type of contamination leads to infectious hepatitis?
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What are the two major types of diseases caused by Salmonella in humans?
What are the two major types of diseases caused by Salmonella in humans?
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Describe the general symptoms of Salmonella infection.
Describe the general symptoms of Salmonella infection.
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What is the optimum growth temperature range for Salmonella?
What is the optimum growth temperature range for Salmonella?
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Where are Salmonella naturally found?
Where are Salmonella naturally found?
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What toxins are produced by Clostridium perfringens, and what are their effects?
What toxins are produced by Clostridium perfringens, and what are their effects?
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What types of foods are typically associated with Salmonella infections?
What types of foods are typically associated with Salmonella infections?
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How does Clostridium perfringens survive in food?
How does Clostridium perfringens survive in food?
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What environmental conditions inhibit the multiplication of Salmonella?
What environmental conditions inhibit the multiplication of Salmonella?
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What are the main characteristics of Yersinia enterocolitica?
What are the main characteristics of Yersinia enterocolitica?
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Describe the habitat of Yersinia enterocolitica.
Describe the habitat of Yersinia enterocolitica.
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What symptoms are associated with yersiniosis and how long do they last?
What symptoms are associated with yersiniosis and how long do they last?
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What food items are commonly associated with Yersinia enterocolitica infections?
What food items are commonly associated with Yersinia enterocolitica infections?
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How are Vibrio spp. primarily transmitted to humans?
How are Vibrio spp. primarily transmitted to humans?
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What is the significance of the Kanagawa phenomenon in Vibrio infections?
What is the significance of the Kanagawa phenomenon in Vibrio infections?
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What are the symptoms of gastroenteritis caused by Kanagawa-positive Vibrio strains?
What are the symptoms of gastroenteritis caused by Kanagawa-positive Vibrio strains?
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Where can Kanagawa-positive Vibrio strains be isolated from?
Where can Kanagawa-positive Vibrio strains be isolated from?
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What is foodborne disease and how does it differ from food intoxication?
What is foodborne disease and how does it differ from food intoxication?
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Identify two ways pathogens can be transmitted through food.
Identify two ways pathogens can be transmitted through food.
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What role do insects play in the transmission of foodborne pathogens?
What role do insects play in the transmission of foodborne pathogens?
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Describe how contaminated wastewater can lead to the transmission of pathogens.
Describe how contaminated wastewater can lead to the transmission of pathogens.
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What is the first stage of transmission for a pathogen to a new host?
What is the first stage of transmission for a pathogen to a new host?
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Name one type of nonbacterial agent of foodborne disease and provide an example.
Name one type of nonbacterial agent of foodborne disease and provide an example.
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What are fomites and how can they contribute to foodborne disease transmission?
What are fomites and how can they contribute to foodborne disease transmission?
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List two bacterial agents commonly associated with foodborne disease.
List two bacterial agents commonly associated with foodborne disease.
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What is the primary etiologic agent of Toxoplasmosis?
What is the primary etiologic agent of Toxoplasmosis?
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How are sporulated and unsporulated oocysts related to Toxoplasmosis?
How are sporulated and unsporulated oocysts related to Toxoplasmosis?
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Identify the definitive hosts for Toxoplasma gondii.
Identify the definitive hosts for Toxoplasma gondii.
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What is Sarcocystosis and its etiologic agent?
What is Sarcocystosis and its etiologic agent?
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What are the implications of congenital Toxoplasmosis infections?
What are the implications of congenital Toxoplasmosis infections?
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What is the causative agent of human trichinellosis?
What is the causative agent of human trichinellosis?
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Describe the lifecycle stage of Trichinella spiralis post-infection.
Describe the lifecycle stage of Trichinella spiralis post-infection.
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How do well-adapted parasites differ in relation to their hosts?
How do well-adapted parasites differ in relation to their hosts?
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What are the two main types of infections caused by tapeworms, and what distinguishes them?
What are the two main types of infections caused by tapeworms, and what distinguishes them?
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Identify the two species of tapeworms mentioned and their corresponding animal hosts.
Identify the two species of tapeworms mentioned and their corresponding animal hosts.
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What is the role of the scolex in tapeworms, and what specific feature does Taenia solium have?
What is the role of the scolex in tapeworms, and what specific feature does Taenia solium have?
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Describe the significance of Capillaria philipiensis and its primary host.
Describe the significance of Capillaria philipiensis and its primary host.
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Explain the mode of transmission for Anisakis simplex and its definitive hosts.
Explain the mode of transmission for Anisakis simplex and its definitive hosts.
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What are mycotoxins, and which fungi produce aflatoxins?
What are mycotoxins, and which fungi produce aflatoxins?
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Identify a mycotoxin that exhibits fungistatic activity and its effects on plants.
Identify a mycotoxin that exhibits fungistatic activity and its effects on plants.
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What is Ochratoxin A, and which organism is it isolated from?
What is Ochratoxin A, and which organism is it isolated from?
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Study Notes
Food Microbiology
- Foodborne disease is any illness resulting from contaminated food, pathogenic bacteria, viruses, parasites, or chemical or natural toxins
- Bacterial agents of foodborne disease include:
- Staphylococcus
- Clostridium
- Listeria
- Salmonella
- Shigella
- Escherichia coli
- Vibrio, Yersinia and Campylobacter
Outline of Foodborne Disease
-
Definition of terms
-
Transmission of pathogens
- Person to person contact (Measles, mumps, tuberculosis)
- By food (chicken skin contaminated with Salmonella)
- By contaminated wastewater (drinking water contaminated by human or animal feces)
- By insects (House flies spreading Salmonella and E. coli O157)
- By fomites (non-living objects like barbed wire causing tetanus)
-
Host invasion
-
Pathogenesis
-
Bacterial agents of foodborne disease
- Staphylococcal intoxication (staphylococcal enterotoxicosis, enterotoxin produced by Staphylococcus aureus)
- Botulism (neurotoxin produced by Clostridium botulinum)
- Salmonellosis (endotoxin of Salmonella spp.)
- Clostridium perfringens illness (enterotoxin released during sporulation of Clostridium perfringens type A)
- Bacillus cereus gastroenteritis (exoenterotoxin released during lysis of Bacillus cereus)
- Enteropathogenic Escherichia coli infection (several stereotypes of E. coli, some invasive and some enterotoxigenic)
- Yersiniosis
- Shigellosis
- Vibrio parahaemolyticus
-
Nonbacterial agents of foodborne disease
- Parasites
- Viruses
- Molds
Foodborne Disease: Bacterial Intoxication
-
Staphylococcal Intoxication
- Caused by ingestion of enterotoxins of Staphylococcus aureus
- Termed as enterotoxin, causing gastroenteritis/intestinal inflammation
- Staphylococcus aureus characteristics: Gram-positive cocci, occur in bunches, nonmotile, noncapsular, nonsporulating
- Facultatively anaerobic
- Preferred conditions/growth of Staphylococcus aureus:
- Ferments carbohydrates
- Causes proteolysis by extracellular proteolytic enzymes
- Temperature range of 7 to 48°C, optimally around 20-37°C
- Water activity (Aw): 0.86
- pH: 4.8
- High salt and sugar (15% concentration); presence of NO2
- Habitat: naturally present in the nose, throat, skin, and hair. Can be present in infections like cuts and facial acne
- Toxin production: produce 7 distinct enterotoxins
- Disease/symptoms of intoxication: Gastroenteritis (2-4 hours after consumption, lasts for 1-2 days)
- Food association: Protein-rich foods, temperature-abused foods, foods handled extensively (ham, corned beef, salami, bacon, barbecued meat, salads, baking products)
-
Botulism
- Results from consumption of food containing the potent toxin botulin of Clostridium botulinum
- Botulin is a neurotoxin creating neurological symptoms
- Clostridium botulinum characteristics
- Gram-positive rods (single cell or small chains)
- Motile
- Obligate anaerobes
- Single terminal spores that are heat resistant (killed at 115°C)
- Growth requirements/conditions: sensitive to low pH, low Aw, and moderately high salt; strains can be proteolytic or nonproteolytic
- Habitat: soil, sewage, mud, sediments; intestinal contents of animals and fishes
-
Other bacterial intoxication details (list of diseases and necessary data are in the provided text)
Foodborne Disease: Bacterial Infection
-
Salmonellosis
- Cause of disease in humans, animals, and birds
- Two major types of diseases: gastroenteritis and typhoid fever
- Caused by eating food contaminated with Salmonella
- Salmonella details/characteristics (list of necessary data are in the provided text)
- Disease/symptoms of Salmonella: Abdominal cramps, diarrhea, nausea, vomiting, chills, fever and prostration (symptoms appear generally at 24-36 hours, and last for 2-3 days)
- Food association: Foods of animal origin (beef, chicken, turkey, pork, eggs, milk and products made from them)
-
Clostridium perfringens illness
- Cause of food poisoning
- Bacterial details (list of necessary data are in the provided text)
- Disease/symptoms of Clostridium perfringens illness:
- Food association: herbs, spices, processed foods (especially those that are incompletely cooked)
-
Bacillus cereus gastroenteritis
- Bacterial details (list of necessary data are in the provided text)
- Disease/symptoms details (list of necessary data are in the provided text)
- Food association: Dried herbs and spices, meat products, soups, vegetables, and sauces
-
Escherichia coli infection
- Bacterial characteristics, Pathogenic types of Escherichia coli (list of necessary data are in the provided text)
- Disease/symptoms details (list of necessary data are in the provided text)
-
Shigellosis
- Bacterial characteristics, Pathogenic types of Shigella (list of necessary data are in the provided text)
- Disease/symptoms details ( list of necessary data are in the provided text)
-
Yersiniosis
- Bacterial characteristics (list of necessary data are in the provided text)
- Disease/symptoms details (list of necessary data are in the provided text)
-
Vibrio parahaemolyticus
- History
- Characteristics of Vibrio parahaemolyticus (list of necessary data are in the provided text)
- Disease/symptoms
- Natural habitat
- Food association
- Prevention & control methods
-
Vibrio cholerae
- History
- Symptoms
- Characteristics of Vibrio cholerae, and methods for prevention and control
-
Viral Infections
- food borne infection caused by ingestion of food containing viruses (survive in gut and attack intestinal flora / bacteriophages / animal viruses).
- FB Virus Transmission (oral-fecal route)
- Infectious hepatitis & Poliomyelitis
- Other viral infections details (list of necessary data are in the provided text)
-
Parasite Infections
- Toxoplasmosis
- Caused by the protozoan Toxoplasma gondii
- Definitive hosts: felids
- Intermediate hosts: Various warm-blooded animals
- Infection details
- Sarcocystosis
- Caused by coccidian parasites
- Involves sarcocysts in striated muscles of different animals
- Infection details
- Trichinosis
- Caused by the parasite worm of the Phylum Nematoda belonging to the genus Trichinella
- Infection involves Trichinella spiralis
- Infection details
- Taeniasis/cysticercosis
- Caused by larval and adult tapeworms
- Infection details / characteristics of Taenia saginata & T solium
- Other parasite infections details (list of necessary data are in the provided text)
- Toxoplasmosis
-
Mold Infections
- Mycotoxins are fungal metabolites
- Aflatoxin - produced by Aspergillus flavus and A. parasiticus
- Patulin
- exhibits strong fungistatic activity and toxic to seeds and seedlings (sugar beet, corn, wheat)
- Ochratoxin - toxic metabolite isolated from Aspergillus ochraceus
- Luteoskyrin
- produced by Penicillium islandicum
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Description
Test your knowledge on foodborne diseases, their transmission, and prevention methods. This quiz covers key pathogens like Salmonella and Clostridium perfringens, focusing on their characteristics and the risks associated with food safety. Enhance your understanding of how to maintain hygiene and prevent infections related to foodborne viruses.