Fluorescence Spectroscopy Quiz

<p>The same fluorescence spectrum is generally observed irrespective of the excitation wavelength</p> Signup and view all the answers

<p>Fluorescence intensity versus wavelength or wavenumber</p> Signup and view all the answers

<p>The nuclei does not have time to adjust</p> Signup and view all the answers

<p>Internal conversion</p> Signup and view all the answers

<p>Difference in physical property of a material when measured along different axes</p> Signup and view all the answers

<p>Rotational diffusion</p> Signup and view all the answers

<p>Emission spectrum of a donor overlapping with the absorption spectrum of an acceptor</p> Signup and view all the answers

<p>Constant illumination and observation</p> Signup and view all the answers

<p>Relationship between anisotropy and rotational diffusion</p> Signup and view all the answers

<p>Molecular information</p> Signup and view all the answers

<p>Distance between the donor and acceptor at which energy transfer efficiency is 50%</p> Signup and view all the answers

<p>Fluorophores that occur naturally</p> Signup and view all the answers

<p>Larger molecules rotate more slowly; smaller molecules rotate faster</p> Signup and view all the answers

<p>FCS is based on temporal fluctuations occurring in a small observed volume</p> Signup and view all the answers

<p>Transfer of energy between fluorophores</p> Signup and view all the answers

<p>Very high obtainable sensitivity</p> Signup and view all the answers

<p>Information about the spectral properties of proteins</p> Signup and view all the answers

<p>To provide spectral properties sensitive to a substance of interest</p> Signup and view all the answers

<p>A decrease in fluorescence intensity</p> Signup and view all the answers

<p>Excitation of fluorophores by the absorption of two or more photons</p> Signup and view all the answers

<p>Molecules emit from the S2 level</p> Signup and view all the answers

<p>Quenching constants and unquenched lifetime based on quencher concentration</p> Signup and view all the answers

<p>Decreases intensity, with collisional quenching occurring upon contact with other molecules</p> Signup and view all the answers

<p>Rapid absorption and longer emission timescales</p> Signup and view all the answers

<p>The directional dependence of fluorescence properties</p> Signup and view all the answers

<p>O2, halogens, amines, and electron-deficient molecules</p> Signup and view all the answers

<p>Measures emitted photons relative to absorbed photons</p> Signup and view all the answers