Flow Cytometry Quiz

Flow cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. It is used in basic research, clinical practice, and clinical trials. Flow cytometry analyzer is an instrument that provides quantifiable data from a sample. The first fluorescence-based flow cytometry device was developed in 1968. Modern flow cytometers are able to analyze many thousands of particles per second, in "real time" and, if configured as cell sorters, can actively separate and isolate particles with specified optical properties at similar rates. Flow cytometry cell sorters have a collection system unlike flow cytometry analyzers. The collection process starts when a sample is injected into a stream of sheath fluid that passes through the flow cell and laser intercepts.Flow cytometry is a technique used to identify cells or particles, and to measure their physical properties. Labels, dyes, and stains can be used for multi-parametric analysis. A wide range of fluorophores can be used as labels, and quantum dots can be used in place of traditional fluorophores. Isotope labeling is used to overcome the fluorescent labeling limit. Cytometric bead array assays are used to measure cellular products such as cytokines, proteins, and other factors. Impedance-based single-cell analysis systems are commonly known as Coulter counters. The technology has applications in molecular biology, pathology, immunology, virology, plant biology, marine biology, and medicine. Flow cytometry is used to measure the efficiency of T-cells in killing target cells in cancer such as leukemia. It has also been used to measure genome sizes.