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Questions and Answers
What is the purpose of a flow cytometry analyzer?
What is the purpose of a flow cytometry analyzer?
- To separate and isolate particles with specified optical properties
- To analyze genome sizes
- To measure cellular products such as cytokines, proteins, and other factors
- To detect and measure physical and chemical characteristics of a population of cells or particles (correct)
When was the first fluorescence-based flow cytometry device developed?
When was the first fluorescence-based flow cytometry device developed?
- 1998
- 1978
- 1968 (correct)
- 1988
What is the main difference between flow cytometry analyzers and cell sorters?
What is the main difference between flow cytometry analyzers and cell sorters?
- Sorters provide quantifiable data from a sample
- Analyzers have a collection system, while sorters do not (correct)
- Analyzers can actively separate and isolate particles with specified optical properties at similar rates
- Sorters are used in basic research, while analyzers are used in clinical practice
What is used to overcome the fluorescent labeling limit in flow cytometry?
What is used to overcome the fluorescent labeling limit in flow cytometry?
What are cytometric bead array assays used to measure?
What are cytometric bead array assays used to measure?
What is the common name for impedance-based single-cell analysis systems?
What is the common name for impedance-based single-cell analysis systems?
What is the range of particles that modern flow cytometers are able to analyze per second?
What is the range of particles that modern flow cytometers are able to analyze per second?
In what fields does flow cytometry have applications?
In what fields does flow cytometry have applications?
What is the purpose of using labels, dyes, and stains in flow cytometry?
What is the purpose of using labels, dyes, and stains in flow cytometry?
What can be used in place of traditional fluorophores in flow cytometry?
What can be used in place of traditional fluorophores in flow cytometry?
Flashcards
Flow Cytometry Purpose
Flow Cytometry Purpose
To detect and measure physical and chemical characteristics of a population of cells or particles.
First Fluorescence Flow Cytometer
First Fluorescence Flow Cytometer
The first fluorescence-based flow cytometry device was developed in 1968.
Analyzers vs. Sorters
Analyzers vs. Sorters
Flow cytometry analyzers have a collection system, whereas cell sorters do not.
Overcoming Fluorescent Limit
Overcoming Fluorescent Limit
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Cytometric Bead Array Assays
Cytometric Bead Array Assays
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Impedance-based Analysis
Impedance-based Analysis
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Modern Flow Cytometer Speed
Modern Flow Cytometer Speed
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Applications of Flow Cytometry
Applications of Flow Cytometry
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Use of Labels and Dyes
Use of Labels and Dyes
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Quantum Dots in Flow Cytometry
Quantum Dots in Flow Cytometry
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Study Notes
Flow cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. It is used in basic research, clinical practice, and clinical trials. Flow cytometry analyzer is an instrument that provides quantifiable data from a sample. The first fluorescence-based flow cytometry device was developed in 1968. Modern flow cytometers are able to analyze many thousands of particles per second, in "real time" and, if configured as cell sorters, can actively separate and isolate particles with specified optical properties at similar rates. Flow cytometry cell sorters have a collection system unlike flow cytometry analyzers. The collection process starts when a sample is injected into a stream of sheath fluid that passes through the flow cell and laser intercepts.Flow cytometry is a technique used to identify cells or particles, and to measure their physical properties. Labels, dyes, and stains can be used for multi-parametric analysis. A wide range of fluorophores can be used as labels, and quantum dots can be used in place of traditional fluorophores. Isotope labeling is used to overcome the fluorescent labeling limit. Cytometric bead array assays are used to measure cellular products such as cytokines, proteins, and other factors. Impedance-based single-cell analysis systems are commonly known as Coulter counters. The technology has applications in molecular biology, pathology, immunology, virology, plant biology, marine biology, and medicine. Flow cytometry is used to measure the efficiency of T-cells in killing target cells in cancer such as leukemia. It has also been used to measure genome sizes.
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Description
Test your knowledge on flow cytometry with our quiz! This technique is used to detect and measure physical and chemical characteristics of cells or particles and has a wide range of applications in various fields. From basic research to clinical practice, flow cytometry provides quantifiable data from a sample and can even separate and isolate particles with specific optical properties. This quiz will cover topics such as fluorescent labeling, cytometric bead array assays, and the efficiency of T-cells in killing cancer cells. So, put your knowledge