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Flow Cytometry: Principles and Components

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Flow Cytometry: Principles and Components

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Questions and Answers

What is the primary function of the fluidics component in a flow cytometer?

To detect fluorescence in cells

To amplify the laser beam

To direct a stream of fluid through the laser beam (correct)

To analyze the data collected by the detection system

Which type of scatter is most indicative of cell size?

Forward scatter (correct)

Backward scatter

Fluorescence

Side scatter

What is the purpose of adding fluorescent antibodies to a patient's sample in flow cytometry?

To amplify the laser beam

To analyze the data collected by the detection system

To detect the presence of antigens on cells (correct)

To remove unbound antibodies

What is the term for the expression of antigens on cells, which can be detected by flow cytometry?

Immunophenotype Signup and view all the answers

What is the wavelength of light emitted by fluorophores in flow cytometry?

Variable wavelength depending on the fluorophore Signup and view all the answers

What is the term for the pulse of photon emission detected by the detection system as a fluorescing cell passes through the interrogation point?

Event Signup and view all the answers

What is the primary principle of flow cytometry?

To analyze cell antigens on viable cells Signup and view all the answers

What is the purpose of monoclonal antibodies in flow cytometry?

To attach to cell antigens and facilitate fluorescent detection Signup and view all the answers

What is the term for a cluster of antibodies that recognize the same antigen?

Cluster of differentiation Signup and view all the answers

What is the primary application of flow cytometry in the diagnosis of acute leukemia?

To identify specific cell antigens and immunophenotype Signup and view all the answers

What is the term for the analysis of cell antigens on viable cells?

Flow cytometry Signup and view all the answers

What is the purpose of forward scatter and side scatter in flow cytometry?

To measure cell size and granularity Signup and view all the answers

What is the characteristic of monoblasts in acute monoblastic leukemia?

Large size with abundant cytoplasm and small granules Signup and view all the answers

Which of the following is a characteristic of Acute Promyelocytic Leukemia (APML)?

High SS (very granular) and CD34 negative Signup and view all the answers

What is the immunophenotype of normal early B lymphoblasts?

CD34+/CD10+/CD19+ Signup and view all the answers