18 Questions
What is the primary function of the fluidics component in a flow cytometer?
To direct a stream of fluid through the laser beam
Which type of scatter is most indicative of cell size?
Forward scatter
What is the purpose of adding fluorescent antibodies to a patient's sample in flow cytometry?
To detect the presence of antigens on cells
What is the term for the expression of antigens on cells, which can be detected by flow cytometry?
Immunophenotype
What is the wavelength of light emitted by fluorophores in flow cytometry?
Variable wavelength depending on the fluorophore
What is the term for the pulse of photon emission detected by the detection system as a fluorescing cell passes through the interrogation point?
Event
What is the primary principle of flow cytometry?
To analyze cell antigens on viable cells
What is the purpose of monoclonal antibodies in flow cytometry?
To attach to cell antigens and facilitate fluorescent detection
What is the term for a cluster of antibodies that recognize the same antigen?
Cluster of differentiation
What is the primary application of flow cytometry in the diagnosis of acute leukemia?
To identify specific cell antigens and immunophenotype
What is the term for the analysis of cell antigens on viable cells?
Flow cytometry
What is the purpose of forward scatter and side scatter in flow cytometry?
To measure cell size and granularity
What is the characteristic of monoblasts in acute monoblastic leukemia?
Large size with abundant cytoplasm and small granules
Which of the following is a characteristic of Acute Promyelocytic Leukemia (APML)?
High SS (very granular) and CD34 negative
What is the immunophenotype of normal early B lymphoblasts?
CD34+/CD10+/CD19+
Which of the following is a characteristic of Acute Myeloid Leukemia (AML) with t(8;21)?
Presence of CD19 aberrant B cell marker
What is the role of CD34 in flow cytometry?
To identify blast cells
What is the characteristic of abnormal B lymphocytes in flow cytometry?
Presence of CD34 and CD19
Understand the basics of flow cytometry, including its components such as fluidics, laser, and detection systems. Learn how it works, including the principles of hydrodynamic focussing and the detection of forward scatter, side scatter, and fluorescence.
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