First Year Experimental Physiology Quiz
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Questions and Answers

How can ion conductances across the nerve fiber membrane be altered experimentally?

They can be the result of electrical current applied through electrodes.

What is the stimulus artifact and why is it significant in nerve conduction studies?

The stimulus artifact is a short period of irregular deflection of the CRO baseline caused by current leakage, indicating the moment the stimulus is applied.

What is the relationship between the velocity of a nerve impulse and action potentials?

The velocity is directly related to the sum of all the action potentials occurring in the individual neurons of the whole nerve.

What else does the nerve conduction velocity reflect besides action potentials?

<p>It reflects changes in ion conductances across the nerve fiber membrane.</p> Signup and view all the answers

What does the latent period represent in nerve conduction studies?

<p>The latent period is an iso potential period following the stimulus artifact that represents the time taken for the impulse to travel from the stimulating electrodes to the recording electrodes.</p> Signup and view all the answers

Which axon characteristic is most dependent on its diameter?

<p>The conduction velocity of its action potential.</p> Signup and view all the answers

Define the firing level in the context of depolarization.

<p>The firing level is the membrane potential at which the rate of depolarization changes from slow to rapid, specifically at -55 mV in nerves.</p> Signup and view all the answers

How does myelination influence nerve conduction velocity?

<p>In myelinated axons, conduction velocity increases as the distance between nodes of Ranvier increases.</p> Signup and view all the answers

What are the components of the spike potential during depolarization?

<p>The spike potential consists of a slow phase and a rapid phase that occurs after reaching the firing level.</p> Signup and view all the answers

What occurs during the repolarization phase of the spike potential?

<p>Repolarization includes a slow phase followed by after-hyperpolarization.</p> Signup and view all the answers

What is the firing level in nerves during depolarization?

<p>-55 mv</p> Signup and view all the answers

Describe the composition of the spike potential.

<p>Spike potential consists of rapid depolarization and rapid repolarization.</p> Signup and view all the answers

What distinguishes saltatory conduction from conduction in unmyelinated fibers?

<p>Saltatory conduction occurs along myelinated fibers, allowing impulses to jump between nodes of Ranvier.</p> Signup and view all the answers

What is the significance of the nodes of Ranvier in myelinated nerve fibers?

<p>Nodes of Ranvier allow for high permeability to Na, facilitating the rapid propagation of nerve impulses.</p> Signup and view all the answers

How is nerve conduction velocity calculated in the example provided?

<p>NCV is calculated using the formula: distance (in mm) / Δ latency (in msec).</p> Signup and view all the answers

What electrodes are used to measure the conduction velocity of the median nerve?

<p>The active electrode is placed on the thenar eminence, and the reference electrode is on the distal phalanx of the thumb.</p> Signup and view all the answers

What are the two components of after potential?

<p>Negative after potential (after depolarization) and positive after potential (hyperpolarization).</p> Signup and view all the answers

What happens immediately after a point in a nerve fiber becomes depolarized?

<p>A repolarization wave starts as the point soon repolarizes after the depolarization wave.</p> Signup and view all the answers

Define a nerve.

<p>A nerve is a collection of nerve cell axons outside the central nervous system.</p> Signup and view all the answers

What classification exists for nerve fibers based on conduction velocity?

<p>Nerve fibers are classified according to their conduction velocity.</p> Signup and view all the answers

How can nerve conduction velocity be measured?

<p>Nerve conduction velocity is measured by the time taken for an impulse to travel between two points along the nerve.</p> Signup and view all the answers

What is the unit of measurement for nerve conduction velocity?

<p>The unit of measurement for nerve conduction velocity is meters per second (m/s).</p> Signup and view all the answers

What instruments are used to study nerve conduction velocity?

<p>Instruments used include a Cathode Ray Oscilloscope (CRO), microelectrodes, and an amplifier.</p> Signup and view all the answers

What does the stimulus artifact indicate in the results?

<p>The stimulus artifact indicates the moment of application of the stimulus on the CRO screen.</p> Signup and view all the answers

What is the latent period in nerve conduction testing?

<p>The latent period is the iso potential period following the stimulus artifact before potential changes appear.</p> Signup and view all the answers

What causes the stimulus artifact?

<p>The stimulus artifact is caused by current leakage from the stimulating electrodes to the recording electrodes.</p> Signup and view all the answers

What determines the compound action potential in a nerve trunk?

<p>The placement of two electrodes within the nerve trunk.</p> Signup and view all the answers

What are the three main waves analyzed in the compound action potential?

<p>$A$, $B$, and $C$.</p> Signup and view all the answers

How is group $A$ of nerve fibers further subdivided?

<p>Into $Aa$, $Aβ$, $Ay$, and $Aδ$.</p> Signup and view all the answers

What is the relationship between the diameter of a nerve fiber and its conduction velocity?

<p>Larger diameter fibers have greater conduction velocity.</p> Signup and view all the answers

What condition may lead to decreased nerve conduction velocity (NCV)?

<p>Demyelination of neurons.</p> Signup and view all the answers

Which type of nerve fibers are myelinated somatic nerves classified under?

<p>Group $A$.</p> Signup and view all the answers

What is a common symptom of Guillain-Barré syndrome?

<p>Weakness or tingling sensation in the legs.</p> Signup and view all the answers

What happens during carpal tunnel syndrome?

<p>The median nerve becomes compressed at the wrist.</p> Signup and view all the answers

Which nerve fibers are considered unmyelinated?

<p>Group $C$ fibers.</p> Signup and view all the answers

What is a common cause of sciatic nerve problems?

<p>A bulging or ruptured spinal disk.</p> Signup and view all the answers

What is the definition of a nerve?

<p>A nerve is a collection of nerve cell axons outside the central nervous system.</p> Signup and view all the answers

What is the significance of the stimulus artifact in a nerve conduction velocity test?

<p>The stimulus artifact indicates the point at which the stimulus is applied on the CRO screen.</p> Signup and view all the answers

How can nerve conduction velocity be measured?

<p>By measuring the latent period and knowing the distance between the electrodes.</p> Signup and view all the answers

What units are used to measure nerve conduction velocity?

<p>Nerve conduction velocity is measured in meters per second (m/s) or milliseconds (ms).</p> Signup and view all the answers

What equipment is used to record nerve impulses during the test?

<p>A Cathode Ray Oscilloscope (CRO) is used to record electrical changes.</p> Signup and view all the answers

What does the latent period represent in nerve conduction studies?

<p>The latent period represents the time taken for the impulse to travel from the stimulating electrodes to the recording electrodes.</p> Signup and view all the answers

What are microelectrodes used for in nerve conduction tests?

<p>Microelectrodes are inserted into the nerve cells to record electrical impulses.</p> Signup and view all the answers

What is the classification of nerve fibers based on?

<p>Nerve fibers are classified according to their conduction velocity.</p> Signup and view all the answers

Study Notes

Experimental Physiology Overview

  • Changes in ion conductance across nerve membranes can occur through applied electrical currents.
  • Nerve conduction velocity (NCV) is directly influenced by action potentials from individual neurons and their ion conductance.

Axon Characteristics

  • Conduction velocity of action potentials is most dependent on axon diameter; larger diameters yield faster transmission.
  • Myelination increases conduction velocity, especially over longer distances between Nodes of Ranvier.
  • Resting and action potentials relate to ion channel density and Na+/K+ pump activity, not diameter.

Experimental Procedure for Nerve Conduction

  • Insert two microelectrodes (one inside, one outside) into a neuron's axon; connect to a cathode ray oscilloscope (CRO) for recording.
  • Stimulate the nerve and observe electrical changes.

Key Observations

  • Stimulus Artifact: Immediate irregular deflection on CRO, indicating stimulus application and caused by current leakage.
  • Latent Period: Iso-potential phase before potential changes; indicates impulse conduction time and can be used to measure NCV.

Action Potential Phases

  • Depolarization: Comprises a slow initial phase and a rapid firing phase, starting at -55 mV.
  • Repolarization: Involves both slow and rapid phases.
  • Spike Potential: Defined by rapid depolarization followed by rapid repolarization, with after-potentials resulting in hyperpolarization or depolarization.

Impulse Conduction Mechanisms

  • Unmyelinated Fibers: Nerve impulses propagate as continuous waves of action potentials; repolarization follows depolarization in the same direction.
  • Myelinated Fibers: Utilize saltatory conduction, where impulses jump between Nodes of Ranvier, accelerating transmission.

Measuring Nerve Conduction Velocity

  • To assess median nerve conduction, electrodes are placed on corresponding muscle and bony landmarks for measurement.
  • Calculate NCV using latency differences and physical distance between stimulation points.

Compound Action Potential

  • Detected using multiple electrodes; consists of different fiber groups (A, B, C), categorized by diameter, myelination, and conduction rate.
  • Larger diameters correspond to higher conduction velocities, with decreased amplitudes indicating potential neuronal degeneration.

Nerve Fiber Classification

  • Ia: Muscle spindle, Aa fibers
  • Ib: Golgi tendon organ, Aa fibers
  • II: Muscle spindle and touch receptors, Aβ fibers
  • III: Pain and cold receptors, Aδ fibers
  • IV: Pain and temperature receptors, C fibers

Clinical Importance

  • NCV tests help diagnose conditions like Guillain-Barré syndrome, carpal tunnel syndrome, herniated disks, and sciatic nerve issues.
  • Decreased NCV is often indicative of nerve damage such as compression or demyelination conditions.

Summary of Nerve Conduction Tests

  • Utilizes CRO, microelectrodes, and amplifiers to measure electrical impulses in nerves.
  • Latent periods and changes in compound action potentials provide insights into nerve function and health.

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Test your knowledge on the fundamentals of experimental physiology with multiple choice questions. Explore concepts such as ion conductances, nerve impulse velocity, and their implications in nerve conduction. Perfect for first-year students aiming to solidify their understanding of physiological principles.

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