Finding Protein Sequence and Gene Coding Region Tutorial
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Questions and Answers

What is the recommended starting point for finding a protein sequence and gene coding region?

Begin the search in Gene not Protein

Where can the coding sequence for a gene be found in the GenBank record?

CDS in The Features section

What is the purpose of making multiple protein sequence alignments with tools like clustalw?

Identify sequence positions where specific amino acids really matter for the structural integrity or function of a given protein

What is PIR (Protein Information Resource) known for?

<p>Offering useful protein analysis tools and databases</p> Signup and view all the answers

What is the main difference in terms of gene density between prokaryotes and eukaryotes?

<p>One gene per 1000 base pair (prokaryotes) versus one gene per 100,000 base pair (eukaryotes)</p> Signup and view all the answers

How do prokaryotic and eukaryotic genomes differ in shape?

<p>Prokaryotes have a single, circular DNA molecule, whereas eukaryotes have multiple linear pieces of DNA called chromosomes</p> Signup and view all the answers

What percentage of the human genome codes for proteins?

<p>Less than 5 percent</p> Signup and view all the answers

Explain the difference between gene sequences and mature mRNA sequences in eukaryotes.

<p>Gene sequences aren't collinear with mature mRNA and protein sequences; only exons are retained in mature mRNA</p> Signup and view all the answers

In what way are protein sequences derived in eukaryotes when compared to prokaryotes?

<p>Protein sequences in eukaryotes are derived by translating the longest ORF, while in prokaryotes, genes exhibit more than one mRNA form</p> Signup and view all the answers

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