12 Questions
What is the purpose of the biomass reaction in a metabolic network reconstruction?
To simulate metabolites consumed during biomass production
How is growth incorporated into the reconstruction in FBA?
By including a biomass reaction that simulates metabolites consumed during biomass production
What defines a system of linear equations in FBA at steady state?
The flux through each reaction given by Sv = 0
What is the purpose of defining an objective function Z = cTv in FBA?
To predict the maximum growth rate
Which analytical technique is non-destructive?
Nuclear magnetic resonance spectroscopy
Which method is known for being demanding in sample treatment due to derivatisation procedures?
GC-MS Mass spectrometry
Which type of model is used in Metabolic Flux Analysis (MFA)?
Steady state model
Which method is an alternative to spectroscopy techniques and ensures efficiency, precision, accuracy, and high sensitivity?
GC-MS Mass spectrometry
Which type of NMR is the most common within NMR analytical techniques?
Proton NMR
What is the main problem associated with Metabolic Flux Analysis (MFA)?
Linear model
Which type of data does Metabolic Flux Analysis (MFA) calculate?
Non-measured extracellular fluxes
What is the focus of GC-MS Mass spectrometry-based methods in sample treatment?
Derivatisation procedures
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