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12 Questions

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Metabolic Network

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Questions and Answers

What is the purpose of the biomass reaction in a metabolic network reconstruction?

To simulate metabolites consumed during biomass production (correct)

To represent the flow of metabolites in and out of the cell

To convert the reconstruction into a mathematical model

To define a system of linear equations

How is growth incorporated into the reconstruction in FBA?

By including a biomass reaction that simulates metabolites consumed during biomass production (correct)

By forming a matrix labeled S with each row representing a metabolite and each column representing a reaction

By using exchange reactions to represent the flow of metabolites in and out of the cell

By defining an objective function Z = cTv to predict the maximum growth rate

What defines a system of linear equations in FBA at steady state?

The incorporation of growth into the reconstruction

The flux through each reaction given by Sv = 0 (correct)

The use of exchange reactions to represent metabolite flow

The conversion of reconstruction into a mathematical model

What is the purpose of defining an objective function Z = cTv in FBA?

To predict the maximum growth rate Signup and view all the answers

Which analytical technique is non-destructive?

Nuclear magnetic resonance spectroscopy Signup and view all the answers

Which method is known for being demanding in sample treatment due to derivatisation procedures?

GC-MS Mass spectrometry Signup and view all the answers

Which type of model is used in Metabolic Flux Analysis (MFA)?

Steady state model Signup and view all the answers

Which method is an alternative to spectroscopy techniques and ensures efficiency, precision, accuracy, and high sensitivity?

GC-MS Mass spectrometry Signup and view all the answers

Which type of NMR is the most common within NMR analytical techniques?

Proton NMR Signup and view all the answers

What is the main problem associated with Metabolic Flux Analysis (MFA)?

Linear model Signup and view all the answers

Which type of data does Metabolic Flux Analysis (MFA) calculate?

Non-measured extracellular fluxes Signup and view all the answers

What is the focus of GC-MS Mass spectrometry-based methods in sample treatment?

Derivatisation procedures Signup and view all the answers