Metabolic Network
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Questions and Answers

What is the purpose of the biomass reaction in a metabolic network reconstruction?

  • To simulate metabolites consumed during biomass production (correct)
  • To represent the flow of metabolites in and out of the cell
  • To convert the reconstruction into a mathematical model
  • To define a system of linear equations

How is growth incorporated into the reconstruction in FBA?

  • By including a biomass reaction that simulates metabolites consumed during biomass production (correct)
  • By forming a matrix labeled S with each row representing a metabolite and each column representing a reaction
  • By using exchange reactions to represent the flow of metabolites in and out of the cell
  • By defining an objective function Z = cTv to predict the maximum growth rate

What defines a system of linear equations in FBA at steady state?

  • The incorporation of growth into the reconstruction
  • The flux through each reaction given by Sv = 0 (correct)
  • The use of exchange reactions to represent metabolite flow
  • The conversion of reconstruction into a mathematical model

What is the purpose of defining an objective function Z = cTv in FBA?

<p>To predict the maximum growth rate (A)</p> Signup and view all the answers

Which analytical technique is non-destructive?

<p>Nuclear magnetic resonance spectroscopy (C)</p> Signup and view all the answers

Which method is known for being demanding in sample treatment due to derivatisation procedures?

<p>GC-MS Mass spectrometry (D)</p> Signup and view all the answers

Which type of model is used in Metabolic Flux Analysis (MFA)?

<p>Steady state model (C)</p> Signup and view all the answers

Which method is an alternative to spectroscopy techniques and ensures efficiency, precision, accuracy, and high sensitivity?

<p>GC-MS Mass spectrometry (D)</p> Signup and view all the answers

Which type of NMR is the most common within NMR analytical techniques?

<p>Proton NMR (A)</p> Signup and view all the answers

What is the main problem associated with Metabolic Flux Analysis (MFA)?

<p>Linear model (B)</p> Signup and view all the answers

Which type of data does Metabolic Flux Analysis (MFA) calculate?

<p>Non-measured extracellular fluxes (C)</p> Signup and view all the answers

What is the focus of GC-MS Mass spectrometry-based methods in sample treatment?

<p>Derivatisation procedures (C)</p> Signup and view all the answers

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