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Evolution of Oxidative Metabolism

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90 Questions

What is the selective advantage created by the increase in atmospheric O2?

Capability to use O2 in energy-producing reactions

What is the energy yield of the complete oxidative breakdown of glucose to CO2 and H2O?

Equivalent to 36-38 molecules of ATP

What is the characteristic of thermacidophiles?

They live in hot environments with low pH values

What is the timeline for the evolution of all forms of life from the original prokaryotes?

3.5-4.0 billion years ago

What is the division of present-day prokaryotes?

Archaebacteria and Eubacteria

What is the likely sequence of events in the evolution of life on Earth?

Prokaryotes, oxidative metabolism, photosynthesis

What is believed to be the primary energy source utilized by the first photosynthetic bacteria?

H2S

What is a by-product of photosynthetic reactions that has a profound impact on the environment?

Free O2

What is the name of the molecule that is used as a source of energy to drive other metabolic reactions?

ATP

What is the name of the metabolic pathway that converts the energy in preformed organic molecules into ATP?

Glycolysis

What is thought to have evolved as a result of the abundance of oxygen in the Earth's atmosphere?

Oxidative metabolism

Approximately how many years ago did the first photosynthetic bacteria evolve?

3 billion years ago

What was the primary source of energy for the transformation of molecules into organic molecules, according to Aleksandr Ivanovich Oparin?

Ultraviolet radiation from the sun

What was the result of the introduction of oxygen into the atmosphere, with respect to Abiogenesis?

It made Abiogenesis impossible

What was the purpose of the Miller-Urey experiment?

To offer evidence that chemical evolution occurred

What was the composition of the primitive atmosphere, according to Oparin's revised hypothesis?

Carbon monoxide, carbon dioxide, nitrogen, hydrogen sulfide, and hydrogen

What was the result of the increase in oxygen in the atmosphere, with respect to the Earth's protection?

The formation of the ozone layer, which shielded the Earth from ultraviolet radiation

What stage of chemical evolution was characterized by the formation of simple organic substances, such as amino acids?

The stage of the primitive environment

What is the primary mechanism by which 'building block' molecules gave rise to macromolecules during chemical evolution?

Polycondensation

According to one theory, where might the 'building block' molecules have originated from?

A combination of the above

What is the term for the synthesis of biochemically important molecules from smaller molecules and chemical elements?

Chemical evolution

What is the name of the scientist who introduced the term 'chemical evolution' and is best known for unraveling the secrets of photosynthesis?

Melvin Calvin

What is thought to have formed the Sun and its planets from the disk-shaped cloud of dust?

All of the above

What is the term for the event that concentrated all matter in a single mass and then blew apart?

The Big Bang

What is the primary reason for the acquisition of aerobic bacteria by anaerobic cells according to the endosymbiotic theory?

To allow the cells to carry out oxidative metabolism

What is the current state of most genes from the original bacteria in the endosymbiotic theory?

Most genes have been incorporated into the nuclear genome of the cell

What is the characteristic shared by both mitochondria and chloroplasts according to the endosymbiotic theory?

They are both similar in size to bacteria and reproduce by dividing in two

What is the hypothesized origin of chloroplasts according to the endosymbiotic theory?

From photosynthetic bacteria

What was the primary advantage of the acquisition of photosynthetic bacteria by cells according to the endosymbiotic theory?

The ability to perform photosynthesis

What is the key factor that drove the selection of cells containing aerobic bacteria and photosynthetic bacteria during evolution?

The increase in atmospheric oxygen

In phospholipid molecules, the phosphate-containing head groups are water-insoluble.

False

The first cell is thought to have arisen by the enclosure of self-replicating DNA in a membrane composed of phospholipids.

False

RNA is capable of catalyzing the breakdown of nucleotides.

False

Phospholipid membranes are impermeable to nutrients and energy from the environment.

False

The bilayer formation of phospholipid membranes occurs with the phosphate-containing head groups on the inside and the hydrocarbon chains on the outside.

False

The first cell was able to self-reproduce and further evolve due to the enclosure of self-replicating RNA and associated molecules in a protein membrane.

False

The term 'chemical evolution' refers to the process of smaller molecules forming into macromolecules through polycondensation.

True

The Calvin cycle is a metabolic pathway that converts energy in preformed organic molecules into ATP.

False

The Big Bang theory proposes that the Earth and its planets were formed from a single, condensed mass of dust.

True

The formation of macromolecules from building block molecules occurred only in the lithosphere of young Earth.

False

The heat produced by the Big Bang led to the compaction of the Earth and the formation of its crust.

True

Melvin Calvin is best known for explaining the process of abiogenesis through the 'Calvin cycle'.

False

The Miller-Urey experiment used a warm flask representing the primordial Earth's atmosphere.

False

The Miller-Urey experiment successfully reproduced the conditions of primitive Earth.

False

The Miller-Urey experiment demonstrated the spontaneous synthesis of organic molecules in a week.

True

The Miller-Urey experiment used enzymes to assist in the synthesis of organic molecules.

False

The condenser in the Miller-Urey experiment was used to heat the atmosphere.

False

The Miller-Urey experiment proved that simple substances can give rise to the chemical building blocks of organisms.

False

Only proteins can direct their self-replication.

False

Protobionts grew and split, and their RNA sequences best suited to environmental parameters did not pass on to offspring.

False

Macromolecules capable of replicating themselves were not capable of evolution.

False

RNA synthesis occurs before protobiont synthesis in the stages of chemical evolution.

False

The diversity of prokaryotic cells came into existence due to natural selection operating on different RNAs.

True

The ability to replicate itself is not a characteristic of macromolecules that evolved into life.

False

Sydney Fox suggested that waves or rain in the primitive environment could’ve provided enough energetic force to form polymers abiotically on the cold, frozen surface of young Earth.

False

Protobionts are capable of reproducing and have all characteristics of living things.

False

RNA molecules were found to function as enzymes in cells by Thomas Cech and his associates in 1950.

False

Variations of RNA molecules could have been produced during replication without mutations and errors.

False

Protobionts are described to have impermeable and non-excitable membranes similar to those found in modern day cells.

False

The final stage of chemical evolution suggests that polymers developed the ability to reproduce and pass genetic information from one generation to the next.

True

In the phospholipid bilayer, the phosphate-containing head groups face inward, while the hydrocarbon chains face outward.

False

The ATP generated in glycolysis is used to drive other metabolic reactions.

True

Photosynthesis is thought to have evolved before glycolysis.

False

The evolution of oxidative metabolism is thought to have occurred in response to the abundance of oxygen in the Earth's atmosphere.

True

The bilayer formation of phospholipid membranes occurs spontaneously in aqueous environments.

True

The development of photosynthesis allowed cells to harness energy from preformed organic molecules.

False

In phospholipid molecules, the phosphate-containing head groups are hydrophilic.

True

Phospholipid membranes are selectively permeable to nutrients and energy from the environment.

True

The Calvin cycle is a metabolic pathway that converts light energy into ATP.

False

The Big Bang theory proposes that the Sun and its planets formed from a disk-shaped cloud of dust.

True

The Miller-Urey experiment demonstrated the spontaneous synthesis of organic molecules from inorganic substances.

True

RNA molecules can catalyze chemical reactions, including the breakdown of nucleotides.

True

Phospholipid molecules have phosphate-containing head groups that are soluble in water.

False

The first cell is thought to have arisen by the enclosure of self-replicating RNA in a protein membrane.

False

Protobionts grew and split, and their RNA sequences best suited to environmental parameters passed on to offspring.

True

Only proteins can direct their self-replication.

False

The bilayer formation of phospholipid membranes occurs with the phosphate-containing head groups on the outside and the hydrocarbon chains on the inside.

False

Nucleic acids cannot serve as templates for synthesis because of nonspecific base pairings between complementary nucleotides.

False

The complete breakdown of glucose to CO2 and H2O through oxidative metabolism yields energy equivalent to 2 molecules of ATP.

False

Phospholipid membranes are impermeable to nutrients and energy from the environment.

False

The bilayer formation of phospholipid membranes occurs with the phosphate-containing head groups on the outside and the hydrocarbon chains on the inside.

False

Oxidative metabolism is a less efficient way of generating energy from organic molecules compared to anaerobic glycolysis.

False

The evolution of oxidative metabolism occurred after the development of photosynthetic reactions.

False

All forms of life are theorized to have evolved from the original eukaryotic cells.

False

Mitochondria and chloroplasts both contain their own DNA that encodes some of their components.

True

The acquisition of aerobic bacteria would have provided cells with the ability to perform photosynthesis.

False

Most genes in the original bacterial DNA are still unique to the chloroplast and mitochondrial DNA.

False

Mitochondria are thought to have evolved from photosynthetic bacteria.

False

The association between cells and aerobic bacteria was advantageous to the cells and allowed them to carry out oxidative metabolism.

True

Chloroplasts and mitochondria are similar in size to eukaryotic cells.

False

Study Notes

Evolution of Metabolic Pathways

  • There is debate on whether oxidative metabolism came before or after photosynthetic reactions
  • Debates for oxidative metabolism BEFORE photosynthesis:
    • Increase in atmospheric O2 created a strong selective advantage for organisms capable of using O2 in energy-producing reactions
    • O2 is a highly reactive molecule, and oxidative metabolism provided a mechanism for generating energy from organic molecules more efficiently than anaerobic glycolysis
    • Complete oxidative breakdown of glucose to CO2 and H2O yields energy equivalent to 36-38 molecules of ATP, compared to 2 ATP formed by anaerobic glycolysis

Evolution through Glycolysis and Photosynthesis

  • Glycolysis provided a mechanism for converting energy from preformed organic molecules (e.g., glucose) to ATP
  • Development of photosynthesis allowed cells to harness energy from sunlight, independent from glucose utilization
  • First photosynthetic bacteria, which evolved over 3 billion years ago, utilized H2S to convert CO2 to organic molecules
  • Use of H2O in photosynthetic reactions produced free O2, making it abundant in Earth's atmosphere
  • Release of O2 led to the development of oxidative metabolism

Prokaryotes

  • First living things on Earth were prokaryotic cells, which exist today as bacteria
  • Prokaryote fossils were first found in 3.4 million-year-old rock in Africa and even older rocks in Australia
  • Some prokaryotes were found to be photosynthetic!
  • All forms of life are theorized to have evolved from the original prokaryotes ~ 3.5-4.0 billion years ago
  • Prokaryotes are divided into two groups: Archaebacteria and Eubacteria
  • Archaebacteria live in extreme environments and were most prevalent in primitive Earth
  • Thermoacidophiles – live in hot sulfur springs (~ 80°C and pH value as low as 2)

Chemical Evolution

  • The term "chemical evolution" was introduced by Melvin Calvin
  • Refers to the synthesis of biochemically important molecules from smaller molecules and certain chemical elements
  • Describes chemical changes on the primitive Earth, giving rise to the first forms of life
  • Building block molecules (e.g., amino acids, fatty acids, or nucleobases) may have been synthesized in the atmosphere, hydrosphere, or lithosphere of young Earth
  • Another theory proposes that these molecules may not have been formed on Earth but brought from outer space by meteorites/comets

Stages of Chemical Evolution

  • Stage 1: Primitive environment
  • Stage 2: Accumulation of organic molecules
  • Stage 3: Protobiont synthesis
  • Stage 4: RNA synthesis
  • Natural selection, operating on different RNAs, would have brought about subsequent evolutionary development

The First Cell

  • Presumed to have arisen by the enclosure of self-replicating RNA in a membrane composed of phospholipids

  • Phospholipids are the basic components of all present-day biological membranes

  • Phospholipid membranes are amphipathic molecules (soluble in water and not soluble in water)

  • The first cell would have maintained self-replicating RNA and associated molecules as a unit, capable of self-reproduction and further evolution### Endo-symbiotic Theory

  • Mitochondria and chloroplasts are thought to have evolved from bacteria, supporting the endo-symbiotic theory.

  • Both mitochondria and chloroplasts are similar to bacteria in size and reproduce by dividing in two.

  • They contain their own DNA, which encodes some of their components.

Evolution of Mitochondria and Chloroplasts

  • Mitochondria evolved from aerobic bacteria, while chloroplasts evolved from photosynthetic bacteria.
  • The acquisition of aerobic bacteria was a natural selection process for anaerobic cells to acquire oxidative metabolism as the atmosphere became more oxygen dense.
  • The acquisition of photosynthetic bacteria provided nutritional independence for cells to perform photosynthesis, utilizing the Earth's ultraviolet rays.

Advantages of Endo-symbiosis

  • The associations between bacteria and cells were advantageous to both parties.
  • Cells containing aerobic (mitochondria) and photosynthetic (chloroplasts) were selected for during evolution.

Genetic Integration

  • Over time, most genes from the original bacteria became incorporated into the nuclear genome of the cell.
  • Few genes remain unique to chloroplast and mitochondrial DNA.

This quiz covers the evolution of oxidative metabolism and its relationship with the increase in atmospheric oxygen. It explores the selective advantages of organisms capable of using oxygen in energy-producing reactions.

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