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Questions and Answers
What is the purpose of developing alternative procedures to replace animal testing?
To reduce the number of animals used in testing and to provide alternative methods for regulatory authorities.
What is the recommended alternative procedure to rabbit blood sugar bioidentity assay?
High-performance liquid chromatography (HPLC).
What is the current trend in reducing the number of animals used in testing insulin?
The number of mice used per sample has been reduced five-fold.
Why is the rabbit blood sugar method considered problematic?
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What is the goal of developing a functional cell-based assay?
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Study Notes
Alternative to Animal Testing
- Developing alternative procedures to replace animal testing aims to reduce animal suffering and promote humane treatment.
Replacing Rabbit Blood Sugar Bioidentity Assay
- The recommended alternative procedure to rabbit blood sugar bioidentity assay is a cell-based assay.
Trend in Reducing Animal Testing in Insulin
- The current trend is to reduce the number of animals used in testing insulin, shifting towards more humane and efficient methods.
Limitations of Rabbit Blood Sugar Method
- The rabbit blood sugar method is considered problematic due to animal welfare concerns and potential variability in results.
Goal of Functional Cell-Based Assay
- The goal of developing a functional cell-based assay is to create a reliable, efficient, and animal-free method for testing insulin.
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Description
Quiz: Evolution of Insulin Testing Methods and Animal Reduction Efforts Discover the advancements in insulin testing and the Wellcome Foundation's efforts to minimize animal usage over the past 30 years. Learn about the progressive methods that have already decreased the number of mice used per sample five-fold.
