Eukaryotic Organelles Overview
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Questions and Answers

What is the primary function of the lysosomes?

  • Break down substrates using hydrolytic enzymes (correct)
  • Produce ATP and metabolic processes
  • Synthesize ribosomal RNA
  • Facilitate protein translation
  • Which structure is primarily involved in the synthesis of ribosomal RNA?

  • Rough Endoplasmic Reticulum
  • Nucleus
  • Golgi apparatus
  • Nucleolus (correct)
  • What is the role of microtubules in the cell?

  • Create pathways for motor proteins and support cilia and flagella (correct)
  • Aid in cell-cell adhesion and maintain cytoskeletal integrity
  • Provide structural protection and aid in muscle contraction
  • Store calcium ions and synthesize lipids
  • Which organelle is involved in ATP production?

    <p>Mitochondria (C)</p> Signup and view all the answers

    What do kinesin motor proteins mediate?

    <p>Transport substrates away from the nucleus (B)</p> Signup and view all the answers

    What is the main function of epithelial tissue?

    <p>Cover the body and line cavities (D)</p> Signup and view all the answers

    Which of the following best describes the smooth endoplasmic reticulum (SER)?

    <p>Involved in lipid synthesis and detoxification (B)</p> Signup and view all the answers

    Which proteins are found in the intermediate filaments of the cytoskeleton?

    <p>Keratin and Desmin (B)</p> Signup and view all the answers

    What type of metabolism does an obligate anaerobe perform?

    <p>Anaerobic metabolism without oxygen (B)</p> Signup and view all the answers

    Which feature distinguishes Gram-positive bacteria from Gram-negative bacteria?

    <p>Gram-positive bacteria have a thick cell wall composed of peptidoglycan (A)</p> Signup and view all the answers

    What is the main function of plasmids in prokaryotes?

    <p>To carry extrachromosomal genetic material (B)</p> Signup and view all the answers

    Which process describes the transfer of genetic material from one bacterium to another via a bacteriophage?

    <p>Transduction (C)</p> Signup and view all the answers

    Which characteristic is true for both Archaea and Eukaryotes?

    <p>They start translation with methionine (B)</p> Signup and view all the answers

    Which of the following distinguishes eukaryotic cells from prokaryotic cells?

    <p>Presence of membrane-bound organelles (C)</p> Signup and view all the answers

    During glycolysis, what is the primary end product generated?

    <p>Pyruvate (D)</p> Signup and view all the answers

    In the lysogenic cycle, what happens to the viral DNA in the host cell?

    <p>It integrates into the host genome (A)</p> Signup and view all the answers

    What cellular structure is involved in chemical signaling and signal transduction?

    <p>Cell membrane (D)</p> Signup and view all the answers

    Which of the following is true regarding retroviruses?

    <p>They create complementary DNA from RNA using reverse transcriptase (D)</p> Signup and view all the answers

    Which statement best describes transposons?

    <p>They can insert or remove themselves from the genome (D)</p> Signup and view all the answers

    During which phase of the cell cycle does DNA replication occur?

    <p>S phase (B)</p> Signup and view all the answers

    What role do cyclins play in the cell cycle?

    <p>They regulate the activity of cyclin-dependent kinases (B)</p> Signup and view all the answers

    What is the primary function of the rough endoplasmic reticulum (ER)?

    <p>Protein synthesis (D)</p> Signup and view all the answers

    Flashcards

    Nucleus

    The control center of the eukaryotic cell, containing DNA organized into chromosomes. It is enclosed by a nuclear membrane with pores, and is the site of transcription (DNA to mRNA).

    Ribosome

    A cellular structure responsible for protein synthesis. In eukaryotes, ribosomes consist of two subunits: 40S and 60S.

    Mitochondria

    Powerhouse of the cell, responsible for energy production through ATP synthesis. Its inner membrane folds into cristae and contains enzymes for the electron transport chain.

    Lysosomes

    Cellular organelles containing hydrolytic enzymes that break down various substrates. They can trigger autolysis (self-destruction) of the cell.

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    Endoplasmic Reticulum (ER)

    A network of interconnected membranes involved in lipid synthesis and protein modification. There are two types: rough ER (RER) and smooth ER (SER).

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    Golgi Apparatus

    Stacked membrane-bound sacs that modify, package, and direct cellular products to their specific locations.

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    Microfilaments

    Thin protein filaments composed of actin, providing structural support, enabling muscle contraction, and forming the cleavage furrow during cytokinesis (cell division).

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    Microtubules

    Hollow tubes made of tubulin, acting as tracks for motor proteins (kinesin and dynein), forming the structure of cilia and flagella, and organizing microtubules in the mitotic spindle during cell division.

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    Prokaryotes

    Unicellular organisms lacking a nucleus and membrane-bound organelles, with a single circular chromosome. They reproduce through binary fission.

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    Gram-positive Bacteria

    Bacteria with a thick cell wall composed of peptidoglycan and lipoteichoic acid, staining purple in Gram staining.

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    Gram-negative Bacteria

    Bacteria with a thin cell wall composed of peptidoglycan and an outer membrane containing phospholipids and lipopolysaccharides, staining pink-red in Gram staining.

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    Obligate Aerobe

    Bacteria that require oxygen for their metabolism and cannot survive without it.

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    Obligate Anaerobe

    Bacteria that cannot survive in the presence of oxygen and use anaerobic metabolism.

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    Facultative Anaerobe

    Bacteria that can survive with or without oxygen, switching between metabolic processes.

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    Aerotolerant Anaerobe

    Bacteria that can survive in the presence of oxygen but use anaerobic metabolism.

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    Transformation (Bacterial Genetics)

    Process where bacteria acquire genetic material from the environment and incorporate it into their genome.

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    Conjugation (Bacterial Genetics)

    Transfer of genetic material from one bacterium to another through a conjugation bridge, typically mediated by sex pili.

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    Transduction (Bacterial Genetics)

    Transfer of genetic material from one bacterium to another via a bacteriophage vector.

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    Lytic Cycle (Bacteriophage)

    The cycle in which a virus replicates within a host cell, producing new virions until the cell lyses.

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    Lysogenic Cycle (Bacteriophage)

    The cycle where the virus integrates into the host genome as a provirus or prophage, replicating alongside the cell.

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    Prions

    Infectious proteins that trigger misfolding of other proteins, leading to decreased solubility and increased resistance to degradation.

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    Viroids

    Plant pathogens that are small circles of complementary RNA, capable of turning off genes.

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    Archaea

    Prokaryotes similar to bacteria in many ways but also share some traits with eukaryotes.

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    Study Notes

    Eukaryotic Organelles

    • Nucleus: Organizes DNA into chromosomes, houses nuclear pores, site of transcription (DNA → RNA).
    • Nucleolus: Subsection of the nucleus where ribosomal RNA (rRNA) is produced.
    • Mitochondria: Site of metabolic processes and ATP production. Inner membrane folds (cristae) contain enzymes for the electron transport chain (ETC). Reproduce independently via binary fission. Can initiate apoptosis.
    • Lysosomes: Contain hydrolytic enzymes for substrate breakdown. Enzyme release leads to autolysis (cell self-destruction).
    • Endoplasmic Reticulum (ER): Series of interconnected membranes continuous with the nuclear envelope. Synthesizes lipids and modifies proteins.
    • Rough ER (RER): Studded with ribosomes; site of protein translation for secretion.
    • Smooth ER (SER): Synthesizes lipids, detoxifies, stores calcium ions.
    • Golgi Apparatus: Stacked membrane-bound sacs; modifies, packages, and directs cellular products to specific locations.
    • Peroxisomes: Contain hydrogen peroxide; break down long-chain fatty acids (beta-oxidation). Involved in phospholipid synthesis, and the pentose phosphate pathway.

    Cytoskeleton Proteins

    • Microfilaments: Composed of actin; provide structural support, cause muscle contractions via interactions with myosin, form the cleavage furrow during cytokinesis.
    • Microtubules: Composed of tubulin; create pathways for motor proteins (kinesin and dynein). Form cilia and flagella, and are part of the mitotic spindle's structure; centrioles (in centrosomes) organize microtubules.
    • Intermediate Filaments: Maintain and strengthen the cytoskeleton, anchor organelles, and facilitate cell-cell adhesion. Examples include keratin and desmin.

    Microtubular Motor Proteins

    • Kinesin: Facilitates anterograde transport (away from the nucleus).
    • Dynein: Facilitates retrograde transport (toward the nucleus).

    Epithelial and Connective Tissues

    • Epithelial Tissue: Covers/lines the body; functions in absorption, secretion, sensation, immunity, desiccation prevention. Parenchyma refers to the epithelial component of an organ (the functional parts).
    • Connective Tissue: Supports the body; provides a scaffold for epithelial cells. The stroma refers to the connective tissue component of an organ (the supportive structure). Examples: bone, cartilage, tendons, ligaments, adipose tissue, blood.

    Prokaryotic Cells (Bacteria & Archaea)

    • Prokaryotic Characteristics: Lacks membrane-bound organelles, single circular DNA, divides via binary fission. Electron transport chain (ETC) occurs on the cell membrane.
    • Gram-Positive Bacteria: Thick peptidoglycan cell wall, lipoteichoic acid; stain purple in Gram staining.
    • Gram-Negative Bacteria: Thin peptidoglycan layer, outer membrane with phospholipids and lipopolysaccharides; stain pink-red in Gram staining.
    • Aerobes, Anaerobes, Facultative Anaerobes, Aerotolerant Anaerobes: Classification based on oxygen requirement for metabolism.

    Prokaryotic Genetics

    • Plasmids: Extrachromosomal DNA; may carry antibiotic resistance or virulence genes. Episomes can integrate into the bacterial chromosome.
    • Transposons: Genetic elements that can move (insert or remove) themselves within the genome (found in both prokaryotes and eukaryotes).
    • Bacterial Genetic Recombination Methods:
    • Transformation: Uptake of environmental DNA.
    • Conjugation: Transfer of DNA between bacteria via a conjugation bridge (sex pilus), often involving plasmids with sex factors in the donor.
    • Transduction: Transfer of DNA using a bacteriophage as a vector.

    Viruses and Subviral Particles

    • Viral Genomes: Can be DNA or RNA, single-stranded or double-stranded.

    • Single-Stranded RNA Viruses: Categories include positive-sense (directly translated) and negative-sense (requires complementary strand synthesis by RNA replicase before translation).

    • Retroviruses: Single-stranded RNA genomes; use reverse transcriptase to synthesize DNA from RNA, which is then integrated into the host genome.

    • RNA Polymerase: Enzyme that synthesizes RNA from DNA during transcription.

    • Bacteriophage Life Cycles:

    • Lytic Cycle: Virus produces new virions (viral particles), leading to cell lysis; virulent bacteria.

    • Lysogenic Cycle: Virus integrates into host genome (provirus or prophage) and replicates with the host cell; provirus may leave the genome.

    • Prions: Infectious proteins that cause misfolding of other proteins, impacting solubility and degradation.

    • Viroids: Plant pathogens, small circular RNA molecules that can turn off genes and cause metabolic/structural changes.

    Prokaryotes vs. Archaea vs. Eukaryotes vs. Viruses

    • Archaea: Similar to Bacteria (prokaryotic features) but also similar to eukaryotes (starting translation with methionine, similar RNA polymerases).
    • Bacteria, Archaea, Eukaryotes, Viruses: Comparison table provided distinguishing their key characteristics (cell type, nucleus, organelles, cell wall, cellular division, chromosome type, cell membrane, and ribosomes).

    Other Cellular Processes

    • Cell Membrane: Phospholipid bilayer, proteins, cholesterol, carbohydrates; passive and active transport mechanisms (diffusion, osmosis, facilitated diffusion, primary/secondary active transport). Endocytosis (phagocytosis, pinocytosis), exocytosis.
    • Cell Cycle: Stages (G1, S, G2, M phase) including checkpoints, cyclins, and cyclin-dependent kinases (CDKs). Phases of mitosis and meiosis comparison.
    • Signal Transduction: Ligand binding to receptors (e.g., G-protein coupled receptors, receptor tyrosine kinases), second messengers (e.g., cAMP, calcium ions, IP3), signal amplification, differing types of cellular signaling (autocrine, paracrine, endocrine, juxtacrine).
    • Metabolism: Glycolysis, citric acid cycle (Krebs cycle), oxidative phosphorylation; role of electron transport chains and chemiosmosis in ATP production in mitochondria.
    • Gene Expression: DNA transcription to RNA; RNA processing (splicing, capping, polyadenylation); mRNA translation to proteins (role of ribosomes, tRNAs, codons); post-translational modifications and regulation (epigenetics, enhancers, silencers).

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    Description

    This quiz covers the major organelles found in eukaryotic cells, including their functions and characteristics. You'll explore the roles of the nucleus, mitochondria, lysosomes, endoplasmic reticulum, and Golgi apparatus. Test your knowledge on how these organelles contribute to cellular processes and the overall function of the cell.

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