Eukaryotic Cell Structure Quiz
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Questions and Answers

What is the primary role of the smooth endoplasmic reticulum?

  • Protein synthesis
  • Ribosome production
  • Lipid synthesis (correct)
  • DNA replication
  • Which of the following structures is part of the double membrane-bound organelles?

  • Lysosome
  • Mitochondrion (correct)
  • Rough Endoplasmic Reticulum
  • Ribosome
  • What is the primary function of the nucleus in eukaryotic cells?

  • Waste breakdown
  • Energy production
  • Control center of the cell containing DNA (correct)
  • Protein synthesis
  • Which component of the cell is responsible for the storage of Ca+2 ions?

    <p>Smooth Endoplasmic Reticulum (B)</p> Signup and view all the answers

    Which element of the cell structure is responsible for maintaining the shape of the nucleus?

    <p>Nuclear lamina (D)</p> Signup and view all the answers

    What is the primary function of intermediate filaments in a cell?

    <p>Providing a scaffold for the cell (C)</p> Signup and view all the answers

    Which microscopy technique is most suitable for observing live cell details?

    <p>Phase contrast microscopy (C)</p> Signup and view all the answers

    What process involves the sequential centrifugation of cells to separate their components?

    <p>Differential centrifugation (C)</p> Signup and view all the answers

    What is the advantage of confocal microscopy over bright-field microscopy?

    <p>Sharper images of the specimen (A)</p> Signup and view all the answers

    What is the final step in the process of preparing tissues for microscopy?

    <p>Cutting into sections (B)</p> Signup and view all the answers

    What distinguishes the structure of cilia from that of flagella?

    <p>Cilia move in an oar-like motion and are shorter than flagella. (C)</p> Signup and view all the answers

    Which of the following is NOT a function of microtubules?

    <p>Cell contraction. (C)</p> Signup and view all the answers

    What is a primary role of lysosomes within the endomembrane system?

    <p>Digestion and waste processing. (A)</p> Signup and view all the answers

    Which component of the cytoskeleton is responsible for the internal transport within the cell?

    <p>Microtubules. (C)</p> Signup and view all the answers

    What describes the mitochondrion's structure?

    <p>Contains a matrix and cristae with circular mtDNA. (C)</p> Signup and view all the answers

    What process do vacuoles primarily facilitate in plant cells?

    <p>Uptake of water by osmosis. (A)</p> Signup and view all the answers

    Which is NOT a part of the endomembrane system?

    <p>Mitochondrion. (B)</p> Signup and view all the answers

    Which of the following does NOT describe a function of the cytoskeleton?

    <p>Digesting cellular waste. (D)</p> Signup and view all the answers

    What is the primary function of the Rough Endoplasmic Reticulum?

    <p>Synthesize proteins for secretion, plasma membrane, or organelles (C)</p> Signup and view all the answers

    What distinguishes the proteins synthesized on free ribosomes from those on bound ribosomes?

    <p>Free ribosome proteins function in cytosol and organelles, while bound ribosome proteins are for export or membrane embedding (C)</p> Signup and view all the answers

    What is the main function of the Golgi apparatus?

    <p>Modify, package, and transport proteins and lipids (A)</p> Signup and view all the answers

    Which of the following is NOT a function of lysosomes?

    <p>Break down fatty acids and toxic compounds (D)</p> Signup and view all the answers

    What is the role of acid hydrolases within the lysosomes?

    <p>To degrade polymers into monomeric subunits (B)</p> Signup and view all the answers

    Which statement about peroxisomes is correct?

    <p>They can degrade fatty acids and toxic compounds but not nucleic acids (B)</p> Signup and view all the answers

    How does the Golgi apparatus exhibit polarity?

    <p>It has distinct cis and trans faces that process proteins differently (D)</p> Signup and view all the answers

    Which enzyme in peroxisomes is responsible for breaking down hydrogen peroxide?

    <p>Catalase (C)</p> Signup and view all the answers

    What structure is directly involved in modifying proteins before they reach the plasma membrane?

    <p>Golgi apparatus (B)</p> Signup and view all the answers

    What component primarily makes up ribosomes?

    <p>Proteins and rRNA (A)</p> Signup and view all the answers

    Which organelle is primarily involved in lipid synthesis and glycogen metabolism?

    <p>Smooth Endoplasmic Reticulum (A)</p> Signup and view all the answers

    What is the primary role of the nucleolus within the nucleus?

    <p>RNA synthesis (C)</p> Signup and view all the answers

    Which component of the cell is characterized by having a double-membrane structure?

    <p>Mitochondrion (D)</p> Signup and view all the answers

    Which structure forms the inner surface of the inner nuclear membrane?

    <p>Nuclear lamina (C)</p> Signup and view all the answers

    Which of the following is NOT a function of the endoplasmic reticulum?

    <p>Production of ATP (D)</p> Signup and view all the answers

    What is a primary function of intermediate filaments?

    <p>Providing structural support for the cell (D)</p> Signup and view all the answers

    Which microscopy technique provides sharper images by reducing blurring?

    <p>Deconvolution fluorescence microscopy (D)</p> Signup and view all the answers

    Which component is primarily separated during the first centrifugation step at 600 g in differential centrifugation?

    <p>Nuclei (A)</p> Signup and view all the answers

    What type of microscopy allows for observing details of live cells and monitoring cell movement?

    <p>Phase contrast microscopy (A)</p> Signup and view all the answers

    What technique can further separate organelle components using sucrose banding?

    <p>Equilibrium density-gradient centrifugation (B)</p> Signup and view all the answers

    What is a key function of the cytoskeleton in cells?

    <p>Maintaining cell shape and facilitating movement (D)</p> Signup and view all the answers

    In which structure would you find the 9+2 microtubule arrangement?

    <p>Cilia and flagella (D)</p> Signup and view all the answers

    What is the primary function of mitochondria in cells?

    <p>Aerobic cellular respiration (C)</p> Signup and view all the answers

    Which of the following correctly describes vacuoles in plant cells?

    <p>They function as storage sites for ions and nutrients (C)</p> Signup and view all the answers

    Which cytoskeletal element is primarily responsible for chromosome movement during cell division?

    <p>Microtubules (D)</p> Signup and view all the answers

    What distinguishes microfilaments from microtubules?

    <p>Microfilaments are polymers of actin, while microtubules are polymers of tubulin. (C)</p> Signup and view all the answers

    Which of the following statements about vesicles is incorrect?

    <p>Vesicles are always involved in lysosomal function. (B)</p> Signup and view all the answers

    What role do microtubules serve in the structure of cilia and flagella?

    <p>They act as the main structural component. (D)</p> Signup and view all the answers

    What occurs to proteins synthesized on bound ribosomes within the endomembrane system?

    <p>They are meant for export or embedding in membranes. (D)</p> Signup and view all the answers

    Which component is primarily responsible for aiding in the folding of nascent polypeptides in the Rough Endoplasmic Reticulum?

    <p>Chaperones (D)</p> Signup and view all the answers

    How do the fates of proteins synthesized by free ribosomes differ from those produced by bound ribosomes?

    <p>Free ribosome proteins are generally for use within the cell. (A)</p> Signup and view all the answers

    What is a main function of the lysosome in eukaryotic cells?

    <p>Degradation of cellular waste and damaged organelles. (C)</p> Signup and view all the answers

    Which sequence accurately describes the secretory pathway for proteins?

    <p>Rough ER → Golgi → lysosome → plasma membrane (C)</p> Signup and view all the answers

    How does the Golgi apparatus modify proteins received from the rough ER?

    <p>By adding carbohydrate groups through glycosylation. (B)</p> Signup and view all the answers

    What is the primary role of peroxisomes in cellular metabolism?

    <p>Degradation of fatty acids and toxic compounds. (B)</p> Signup and view all the answers

    What distinguishes the lysosomal lumen's acidity from other cellular compartments?

    <p>It has a low pH of approximately 4.8. (B)</p> Signup and view all the answers

    Which type of ribosomal subunits make up a functional eukaryotic ribosome?

    <p>40S and 60S (C)</p> Signup and view all the answers

    Which organelle is primarily described as the 'post office' of the cell?

    <p>Golgi Apparatus (B)</p> Signup and view all the answers

    Flashcards

    Eukaryotic Cell Nucleus

    Largest organelle in animal cells, containing most of the cell's DNA, surrounded by a double membrane.

    Endoplasmic Reticulum (ER)

    A single-membrane organelle with an extensive network of sacs, continuous with the outer nuclear membrane.

    Smooth ER

    Part of the ER, lacking ribosomes, involved in calcium storage, glycogen metabolism, and lipid synthesis.

    Major Eukaryotic Cell Components

    Plasma membrane (outer boundary), internal membranes/organelles, and cytoskeleton (internal structure).

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    Nuclear Function

    The nucleus controls the cell's activities because it holds the DNA, which directs protein synthesis (and hence virtually all cellular activities).

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    Cytokinesis cleavage furrow

    The indentation of the cell membrane that forms during cytokinesis to divide the cytoplasm.

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    Cell Fractionation

    A method to separate different cell components based on size and density. Grind, centrifuge, and separate.

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    Differential Centrifugation

    Cell fractionation technique using varying centrifugal speeds to separate cellular components based on size and density.

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    Equilibrium Density Gradient Centrifugation

    A technique that separates components in a gradient based on density differences.

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    Bright-field Microscopy

    A basic type of microscopy that uses light to view specimens.

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    Endomembrane System Components

    The endomembrane system includes the nuclear envelope, endoplasmic reticulum membranes, Golgi apparatus, vesicles, and lysosomes. These organelles work together to modify, package, and transport proteins and lipids within the cell.

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    Mitochondrion Structure

    Mitochondria have two membranes (outer and inner). The inner membrane folds into cristae, creating a larger surface area for cellular respiration. The matrix is the space within the inner membrane.

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    Mitochondrial Function

    Mitochondria are the site of aerobic cellular respiration, where energy is produced for the cell. This is a critical energy-producing process.

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    Vesicles/Vacuoles

    Membranous sacs involved in storage, transport, (endocytosis and exocytosis) and other roles. Plant vacuoles are large sacs for storage or water uptake.

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    Cytoskeleton Components

    The cytoskeleton is a network of protein fibers (microtubules, microfilaments, and intermediate filaments) that gives the cell its shape, structure, allows movement, and facilitates internal transport.

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    Microtubule Function

    Microtubules are involved in cell division (chromosome movement), movement of intracellular components (vesicles), and form structures like cilia and flagella.

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    Cilia and Flagella

    Whip-like projections from the cell that are used for movement. Short cilia move in an oar-like motion; longer flagella move in whip-like motions.

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    Microfilament Function

    Microfilaments, made of actin, play a role in cell contraction, like muscle cells, and other cellular movements.

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    Rough ER function

    Initial processing of proteins destined for export, plasma membrane, or other organelles like ER, Golgi, and lysosomes.

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    Ribosome function

    Site of protein synthesis (translation) using rRNA and proteins.

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    Bound ribosomes vs. free ribosomes

    Bound ribosomes synthesize proteins for export, plasma membrane, or organelles, while free ribosomes synthesize proteins for cytosolic use or other organelles.

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    Golgi apparatus function

    Post-office of the cell; modifies, sorts, and packages proteins received from the ER for delivery to other locations.

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    Secretory pathway

    Movement of proteins synthesized by bound ribosomes, through the rough ER, Golgi, and vesicles leading to destinations like plasma membrane or lysosomes.

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    Lysosome function

    Digestive/recycling system of the cell; contains hydrolytic enzymes that break down cellular waste and debris.

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    Peroxisome function

    Degrades fatty acids and other toxic compounds using oxidases; also breaks down H2O2 using catalase.

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    Endomembrane system

    A group of organelles and membranes in eukaryotic cells responsible for modifying, packing and transporting proteins and lipids.

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    Protein Folding

    Newly formed proteins often require assistance to fold correctly, supported by chaperones in the ER lumen.

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    Polarity of Golgi.

    Golgi apparatus has different sides (cis and trans faces) with specialized functions.

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    What does the ER lumen connect to?

    The ER lumen is continuous with the inter-membrane space of the nucleus, creating a connected internal space within the cell.

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    Smooth ER Functions

    The smooth ER performs various functions including calcium storage, glycogen metabolism, and lipid synthesis.

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    What is the major difference between Rough ER and Smooth ER?

    Rough ER is studded with ribosomes, involved in protein synthesis, while Smooth ER lacks ribosomes and performs other functions like lipid synthesis and calcium storage.

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    What are the main components of a eukaryotic cell?

    Eukaryotic cells are made up of three major components: Plasma Membrane, Internal Membranes & Organelles, and Cytoskeleton.

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    Free Ribosomes vs. Bound Ribosomes

    Free ribosomes synthesize proteins for use within the cytosol, nucleus, mitochondria, chloroplasts, and peroxisomes. Bound ribosomes synthesize proteins for export, membrane embedding, or delivery to other organelles.

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    What happens to proteins made on bound ribosomes?

    Proteins made on bound ribosomes will be sent to the ER lumen for further processing and folding, then packaged into vesicles and sent to their final destination, which could be outside the cell or another organelle.

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    What happens to proteins in the Golgi?

    Proteins received from the ER undergo modification and sorting in different Golgi compartments, depending on their final destination. For example, they might be glycosylated (sugars added) or have other modifications.

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    What is the connection between bound ribosomes, rough ER, the Golgi, and lysosomes?

    Proteins synthesized by bound ribosomes travel through the rough ER for initial processing, then to the Golgi apparatus for further modification and packaging before being transported by vesicles to their final destination, which may include lysosomes for degradation.

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    Cytoskeleton

    A network of protein fibers that provides structural support, facilitates movement, and anchors cellular components.

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    Microtubules

    Polymers of α and β tubulin that form long, hollow tubes essential for cell division, intracellular transport, and cilia/flagella structure.

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    Microfilaments

    Polymers of actin that are thinner and more flexible, playing roles in cell contraction and other movements.

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    Cleavage Furrow Formation

    The indentation of the cell membrane that forms during cytokinesis, ultimately dividing the cytoplasm into two daughter cells. This process is driven by the contraction of a ring of microfilaments, often made of actin.

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    Cytoplasmic Streaming

    The directed flow of cytoplasm within a cell, often driven by the movement of microfilaments or microtubules. This movement helps distribute nutrients, organelles, and other essential materials throughout the cell.

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    Intermediate Filament Function

    Intermediate filaments provide structural support and mechanical strength to cells, helping maintain cell shape and anchoring organelles. They also play a role in forming the nuclear lamina, a mesh-like structure lining the inner surface of the nuclear membrane.

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    Phase Contrast Microscopy

    A type of microscopy that enhances the contrast of transparent specimens by converting differences in refractive index into differences in brightness, allowing visualization of details in live cells without staining.

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    Confocal Microscopy

    A specialized fluorescence microscopy technique that uses lasers and pinholes to create sharper images of thick specimens by reducing blurring and focusing on a single plane at a time.

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    Study Notes

    Eukaryotic Cellular Structure

    • Eukaryotic cells have various components, including nuclei, microtubules, Golgi apparatus, actin fibers, and mitochondria. These parts perform different functions essential for cellular activities.

    Substructures of Animal & Plant Cells

    • Diagrams show animal and plant cells with labeled organelles, including numbered items: plasma membrane, mitochondria, lysosomes, nuclear envelope, nucleolus, nucleus, smooth ER, rough ER, Golgi complex, secretory vesicles, peroxisomes, cytoskeletal fibers, microvilli, cell wall, vacuole, and chloroplast.

    Major Components of Eukaryotic Cells

    • Plasma membrane: 700 μm².
    • Internal membranes & organelles: 7,000 μm².
    • Cytoskeleton: 94,000 μm².

    Organelles

    • Single-Membrane Bound: Smooth Endoplasmic Reticulum, Rough Endoplasmic Reticulum, Lysosome, Golgi Apparatus, Peroxisome, Vacuoles & Vesicles.
    • Double-Membrane Bound: Nucleus, Mitochondrion, Chloroplast.
    • Non-Membrane Bound: Ribosomes, Proteasomes, Cytoskeleton elements, Cilia & Flagella, Centrosomes, Basal Bodies.

    Nucleus

    • Typically the largest organelle in animal cells.
    • Considered the "control center" of the cell.
    • Contains most of the cell's DNA.
    • Has a double-membrane structure: outer nuclear membrane, inter-membrane space, inner nuclear membrane.
    • Contains nucleoplasm, where the nucleolus synthesizes rRNA.
    • Also includes a nuclear lamina composed of lamin proteins lining the inner nuclear membrane surface.
    • Contains nuclear pore complexes (NPCs).

    Endoplasmic Reticulum (ER)

    • A single-membrane bound organelle, extensive network of closed, flattened membrane-bound sacs.
    • The ER membrane is continuous with the outer nuclear membrane, and its lumen is continuous with the intermembrane space of the nucleus.
    • Divided into two components: rough ER (RER) and smooth ER (SER).

    Rough ER

    • Immediately adjacent to the nucleus.
    • Studded with ribosomes on its cytosolic surface, which synthesize proteins.
    • Site of initial protein processing, for proteins exported from the cell, embedded in the plasma membrane, or destined for the Golgi apparatus and lysosomes.
    • Nascent polypeptides associate with Rough ER membrane.
    • Chaperones assist protein folding; enzyme modifications occur.

    Ribosomes

    • Sites of protein synthesis (translation), consist of rRNA and proteins.
    • Ribosomes may be free (in cytosol) or bound to the rough ER.
    • A functional ribosome consists of two subunits (large and small), designated by S values.

    Fates of Proteins Synthesized on Bound or Free Ribosomes

    • Proteins synthesized on bound ribosomes are destined for: export from the cell, plasma membrane embedding, or the ER, Golgi, or lysosomes.
    • Proteins synthesized on free ribosomes are for use in cytosol, nucleus, mitochondria, chloroplasts, peroxisomes.

    Golgi Apparatus

    • Series of flattened membrane-bound sacs (cisternae).
    • Acts as the cell's "post office," receiving, modifying, sorting, and packaging proteins and lipids.
    • A polarity exists, with cis, medial, and trans Golgi faces.
    • Contains different luminal enzymes for protein modifications. Proteins arrive from rough ER in transport vesicles.

    Secretory Pathway

    • Movement of proteins from bound ribosomes through rough ER, transport vesicles, cis-Golgi, medial-Golgi, trans-Golgi, transport vesicles, plasma membrane, or lysosomes.

    Lysosome

    • Animal cell's "digestive system" and "garbage disposal system."
    • Contains hydrolytic enzymes (acid hydrolases).
    • Breaks down polymers into smaller subunits within the low pH (around 4.8) lysosomal lumen.
    • V-class proton pumps bring in H+ and Cl- channels bring in Cl-.
    • Digest materials taken up by the cell, digest damaged or aged organelles (autophagy), and are involved in apoptosis.
    • Several human diseases are caused by defects in specific lysosomal enzymes, like Tay-Sachs disease, affecting the breakdown of glycolipids and leading to their accumulation.

    Phagocytosis

    • Process where a cell engulfs a pathogen or other large particle. Phases involve formation of a phagosome, merging with a lysosome, and digestion of contents.

    Cellular Structures Delivering Materials to Lysosomes

    • Endocytosis, phagocytosis, and autophagy are processes where material is brought into a cell and delivered to lysosomes for breakdown and recycling.

    Peroxisomes

    • Degrade fatty acids and toxic compounds.
    • Contain oxidases which leverage oxygen (O2) to oxidize organic substances and produce hydrogen peroxide (H₂O₂).
    • Also contains catalase, which breaks down H₂O₂ into water (H₂O) and oxygen (O₂).
    • Self-assembling organelles, "life span" of a day.
    • Involved in peroxisomal biogenesis

    Endomembrane System

    • A group of membranes and organelles in eukaryotic cells that work together to modify, package, and transport lipids and proteins.
    • Includes the nuclear envelope, endoplasmic reticulum membranes, the Golgi apparatus, vesicles, and lysosomes.

    Mitochondrion

    • Double-membrane bound organelle.
    • Basic structure: outer membrane, intermembrane space, inner membrane with infoldings called cristae, and a lumen termed the matrix.
    • Contains circular mtDNA, granules, and mitoribosomes.
    • Site of aerobic cellular respiration, referred to as the "powerhouse of the cell".

    Vesicles & Vacuoles

    • General term for a variety of single-membrane bound sacs.
    • Vacuoles (plant cells): storage site for ions and nutrients; uptake of water by osmosis creates pressure to push membrane against cell wall.

    Cytoskeleton Elements

    • Responsible for maintaining and changing cell shape, internal transport, anchoring internal and external components, cell movement, and chromosome movement during cell division.
    • Includes microtubules (polymers of a and ẞ tubulin), microfilaments (polymers of actin), and intermediate filaments (various proteins).

    Functions of Microtubules

    • Move chromosomes during cell division.
    • Form centrioles, basal bodies, and centrosomes.
    • Serve as tracks for vesicle and vacuole movement.
    • Provide internal scaffolding for eukaryotic cilia and flagella.

    Cilia & Flagella

    • Locomotor structures (cytoplasmic extensions) that differ in length.
    • Cilia are shorter, moving in an oar-like motion.
    • Flagella are longer, moving in a whip-like manner.
    • They share a 9+2 microtubule arrangement.

    Functions of Microfilaments

    • Involved in cell contraction (like cleavage furrow formation during cytokinesis).
    • Anchor for extracellular elements.
    • Play a role in cytoplasmic streaming.

    Functions of Intermediate Filaments

    • Form a scaffold for the cell.
    • Part of the internal lining of the inner nuclear membrane.

    Size Range of Cells

    • A size chart showing the relative sizes of viruses, bacterial cells, typical human cells, and various cellular components such as mitochondria, lysosomes, ribosomes, plasma membranes, and even a hydrogen atom, ranging from thousands of micrometers down to less than 0.0001 micrometers.

    Different Types of Microscopes

    • Techniques like bright-field microscopy, phase contrast microscopy, differential interference contrast microscopy, fluorescence microscopy, confocal microscopy, offer different ways to view biological samples and structures.

    Preparing Tissues for Microscopy

    • Tissues are fixed, embedded into blocks, and are cut into sections using techniques like microtomes to view sections of tissue with a microscope.

    Deconvolution Fluorescence Microscopy

    • Deconvolution microscopy sharpens fluorescent images by reducing blurring effects due to light and depth-of-field.

    Differential Centrifugation

    • A method to isolate different cellular components by separating them based on their density and size through centrifugation at different speeds and times. Components like nuclei, mitochondria, plasma membranes, ribosomes, and soluble cytoplasm are separated.

    Cell Fractionation and Differential Centrifugation

    • Technique for separating components through centrifugal forces.

    Equilibrium Density-Gradient Centrifugation

    • Method to further separate organelles by density differences within a sucrose gradient medium. Examples of isolated organelles include lysosomes, mitochondria, and peroxisomes, separated by density.

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