Estimation of Fasting Serum Glucose Quiz

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What is the method principle for the quantitative determination of glucose in serum or plasma?

Oxidation of D-glucose to gluconic acid and hydrogen peroxide (correct)

Oxidation of gluconic acid to D-glucose and hydrogen peroxide

Reduction of hydrogen peroxide to water and oxygen

Reduction of gluconic acid to D-glucose and oxygen

What is the normal range for fasting glucose in adults?

3.0 – 6.0 mmol/L

3.9 – 5.8 mmol/L (correct)

4.5 – 6.5 mmol/L

5.0 – 7.0 mmol/L

What is the consequence of not separating serum or plasma within 20 minutes?

Loss of serum glucose due to glycolysis (correct)

Increase in serum glucose concentration

No impact on serum glucose levels

Decrease in serum glucose concentration

What is the purpose of incubating the mixture at 37°C for 15 minutes?

To allow the reaction to take place Signup and view all the answers

How is the concentration of the sample calculated?

By dividing the absorbance of the sample by the absorbance of the standard and multiplying by the concentration of the standard Signup and view all the answers

What is the principle of the method used for the quantitative determination of glucose in serum or plasma?

Oxidation of D-glucose to gluconic acid and hydrogen peroxide Signup and view all the answers

What is the consequence of not separating serum or plasma within 20 minutes?

Serum glucose loss due to glycolysis Signup and view all the answers

What is the normal range for fasting glucose in adults?

3.9 – 5.8 mmol/L Signup and view all the answers

What is the purpose of incubating the mixture at 37°C for 15 minutes?

To allow the reaction to take place Signup and view all the answers

How is the concentration of the sample calculated?

By dividing the sample absorbance by the standard absorbance and multiplying by the standard concentration Signup and view all the answers

Estimation of Fasting Serum Glucose Quiz

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Questions and Answers

What is the method principle for the quantitative determination of glucose in serum or plasma?

What is the normal range for fasting glucose in adults?

What is the consequence of not separating serum or plasma within 20 minutes?

What is the purpose of incubating the mixture at 37°C for 15 minutes?

How is the concentration of the sample calculated?

What is the principle of the method used for the quantitative determination of glucose in serum or plasma?

What is the consequence of not separating serum or plasma within 20 minutes?

What is the normal range for fasting glucose in adults?

What is the purpose of incubating the mixture at 37°C for 15 minutes?

How is the concentration of the sample calculated?

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