Estimation of Fasting Serum Glucose Quiz
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Questions and Answers

What is the method principle for the quantitative determination of glucose in serum or plasma?

  • Oxidation of D-glucose to gluconic acid and hydrogen peroxide (correct)
  • Oxidation of gluconic acid to D-glucose and hydrogen peroxide
  • Reduction of hydrogen peroxide to water and oxygen
  • Reduction of gluconic acid to D-glucose and oxygen

    • What is the normal range for fasting glucose in adults?

  • 3.0 – 6.0 mmol/L
  • 3.9 – 5.8 mmol/L (correct)
  • 4.5 – 6.5 mmol/L
  • 5.0 – 7.0 mmol/L

    • What is the consequence of not separating serum or plasma within 20 minutes?

  • Loss of serum glucose due to glycolysis (correct)
  • Increase in serum glucose concentration
  • No impact on serum glucose levels
  • Decrease in serum glucose concentration

    • What is the purpose of incubating the mixture at 37°C for 15 minutes?

    <p>To allow the reaction to take place</p> Signup and view all the answers

    How is the concentration of the sample calculated?

    <p>By dividing the absorbance of the sample by the absorbance of the standard and multiplying by the concentration of the standard</p> Signup and view all the answers

    What is the principle of the method used for the quantitative determination of glucose in serum or plasma?

    <p>Oxidation of D-glucose to gluconic acid and hydrogen peroxide</p> Signup and view all the answers

    What is the consequence of not separating serum or plasma within 20 minutes?

    <p>Serum glucose loss due to glycolysis</p> Signup and view all the answers

    What is the normal range for fasting glucose in adults?

    <p>3.9 – 5.8 mmol/L</p> Signup and view all the answers

    What is the purpose of incubating the mixture at 37°C for 15 minutes?

    <p>To allow the reaction to take place</p> Signup and view all the answers

    How is the concentration of the sample calculated?

    <p>By dividing the sample absorbance by the standard absorbance and multiplying by the standard concentration</p> Signup and view all the answers

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