Estimation of Amino Acids with Sorenson's Formal Titration
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Questions and Answers

What is the purpose of using excess formaldehyde in the estimation of glycine purity?

  • To prevent the formation of zwitterions
  • To block the amino group by forming methylene glycine (correct)
  • To enhance the reaction with NaOH
  • To neutralize the glycine solution

Why is it important for the formaldehyde solution and glycine solutes to be brought to the same pH before mixing?

  • To maintain stability of the cation formed
  • To prevent zwitterion formation
  • To avoid interference with formic acid
  • To ensure optimal reaction conditions (correct)

Why can't amino acids like glycine be estimated by direct titration with standard alkali solution?

  • Neutralization of amino acids by alkali solution
  • Formation of unstable compounds with alkali solution
  • Inability of amino acids to react with alkali solution
  • Amino group interference at the end point (correct)

What is the role of NaOH in the estimation process of glycine purity?

<p>To convert glycine into a form that can react with formaldehyde (D)</p> Signup and view all the answers

What is the state of glycine in aqueous solution before reacting with formaldehyde and NaOH?

<p>&quot;Zwitterion&quot; (B)</p> Signup and view all the answers

What is the purpose of using excess formaldehyde in the estimation of glycine purity?

<p>To block the amino group by forming methylene glycine (B)</p> Signup and view all the answers

What is the role of NaOH in the estimation process of glycine purity?

<p>To convert glycine into a stable cation (C)</p> Signup and view all the answers

Why can't amino acids like glycine be estimated by direct titration with standard alkali solution?

<p>Amino group interference at the end point (A)</p> Signup and view all the answers

What is the state of glycine in aqueous solution before reacting with formaldehyde and NaOH?

<p>&quot;Zwitterion&quot; incapable of reacting with formaldehyde (C)</p> Signup and view all the answers

Why is it important for the formaldehyde solution and glycine solutes to be brought to the same pH before mixing?

<p>To prevent undesirable side reactions during titration (B)</p> Signup and view all the answers

Flashcards

Formaldehyde's role in glycine purity

Blocks the amino group, forming methylene glycine, enabling accurate estimation.

Equal pH for formaldehyde and glycine

Ensures optimal reaction conditions, preventing unwanted reactions during titration.

Direct titration of amino acids

Not possible due to interference of the amino group at the endpoint of the reaction.

NaOH's role in glycine estimation

Converts glycine to a reactive form, enabling its reaction with formaldehyde.

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Glycine's aqueous state (before reaction)

Glycine exists as a 'zwitterion' in an aqueous solution before reaction.

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Glycine's role in zwitterion form

A zwitterion form of glycine is unable to react with formaldehyde until it is converted.

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Formaldehyde excess purpose

Blocks the amino group in glycine, leading to a better determination of the amount of glycine.

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NaOH's effect on glycine

Converts glycine into a stable cation form for better reaction with formaldehyde.

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Amino group interference

The amino group from glycine causes inaccuracy in titration results.

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Zwitterion and reaction

Glycine, in its zwitterion form, is unable to react until it is modified.

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Study Notes

Estimation of Glycine Purity

  • Excess formaldehyde is used to ensure complete reaction with glycine, allowing for accurate estimation of glycine purity.
  • The formaldehyde solution and glycine solutes must be brought to the same pH before mixing to prevent uneven reaction rates and ensure accurate results.

Titration of Amino Acids

  • Amino acids like glycine cannot be estimated by direct titration with standard alkali solution because they do not react with alkali in a straightforward manner.

Role of NaOH

  • NaOH is used to convert glycine into its anionic form, allowing it to react with formaldehyde and facilitating the estimation process.

State of Glycine in Aqueous Solution

  • In aqueous solution, glycine exists in its zwitterionic form, which must be converted to its anionic form by NaOH before reacting with formaldehyde.

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Description

Learn about the estimation of amino acids, such as glycine, using Sorenson's Formal titration method. This quiz covers the identification of percentage purity of a glycine sample and the determination of the amount of amino acid present in a given solution.

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