Podcast
Questions and Answers
Which of the following is NOT a mode of detection commonly used in molecular recognition?
Which of the following is NOT a mode of detection commonly used in molecular recognition?
- Chemiluminescence (correct)
- Fluorescence
- Absorption
- Radioactivity
What is the primary advantage of using 32P as a radioisotope for detecting DNA and RNA?
What is the primary advantage of using 32P as a radioisotope for detecting DNA and RNA?
- Low cost
- Long half-life
- High emission energy (correct)
- Easy availability
Which radioactive isotope is commonly used to label proteins and nucleic acids?
Which radioactive isotope is commonly used to label proteins and nucleic acids?
- 14C
- 32P
- 35S (correct)
- 3H
What are the two modes of detection commonly used to probe for the presence of DNA, RNA, or protein?
What are the two modes of detection commonly used to probe for the presence of DNA, RNA, or protein?
Which of the following radioisotopes has the longest half-life?
Which of the following radioisotopes has the longest half-life?
Which of the following organs is most vulnerable to DNA damage from breathing in radioactive material?
Which of the following organs is most vulnerable to DNA damage from breathing in radioactive material?
What is the maximum specific activity of 3H?
What is the maximum specific activity of 3H?
Why isn't bacterial DNA digested by restriction enzymes produced by bacteria?
Why isn't bacterial DNA digested by restriction enzymes produced by bacteria?
Which technique is used to detect and localize specific RNA targets in cells and tissue samples?
Which technique is used to detect and localize specific RNA targets in cells and tissue samples?
What is the purpose of a Northern blot?
What is the purpose of a Northern blot?
What is the main difference between Southern blot and Northern blot?
What is the main difference between Southern blot and Northern blot?
What is the purpose of DNA-FISH?
What is the purpose of DNA-FISH?
Which of the following is true about restriction enzymes?
Which of the following is true about restriction enzymes?
Which type of restriction enzyme cuts within its recognition sequences?
Which type of restriction enzyme cuts within its recognition sequences?
What is the purpose of a Southern blot?
What is the purpose of a Southern blot?
What is the purpose of restriction fragment length polymorphism (RFLP) technique?
What is the purpose of restriction fragment length polymorphism (RFLP) technique?
Which of the following is true about DNA arrays?
Which of the following is true about DNA arrays?
What are the two types of light chains in antibodies?
What are the two types of light chains in antibodies?
Which enzyme cleaves an antibody into three fragments?
Which enzyme cleaves an antibody into three fragments?
What is the difference between complete antigens and incomplete antigens?
What is the difference between complete antigens and incomplete antigens?
Which type of epitope is common in fibrous proteins?
Which type of epitope is common in fibrous proteins?
Which type of epitope is common in globular proteins and helical structures?
Which type of epitope is common in globular proteins and helical structures?
What enables antibodies to distinguish between antigens that are chemically very similar?
What enables antibodies to distinguish between antigens that are chemically very similar?
What type of immunoassay is better suited for large analyte molecules?
What type of immunoassay is better suited for large analyte molecules?
Which enzyme is commonly linked to the secondary antibody in Western Blot detection?
Which enzyme is commonly linked to the secondary antibody in Western Blot detection?
What is the preferred method of detection in Western Blot using substrates?
What is the preferred method of detection in Western Blot using substrates?
How can the half-life of a protein be determined in a pulse-chase experiment?
How can the half-life of a protein be determined in a pulse-chase experiment?
What is the equation to calculate the half-life (t1/2) of a protein in a pulse-chase experiment?
What is the equation to calculate the half-life (t1/2) of a protein in a pulse-chase experiment?
What does a biosensor consist of?
What does a biosensor consist of?
What is the equation for a biosensor?
What is the equation for a biosensor?
Which of the following is NOT a mode of detection commonly used in molecular recognition?
Which of the following is NOT a mode of detection commonly used in molecular recognition?
What is the maximum specific activity of 32P?
What is the maximum specific activity of 32P?
What is the purpose of DNA-FISH?
What is the purpose of DNA-FISH?
Which enzyme cleaves an antibody into three fragments?
Which enzyme cleaves an antibody into three fragments?
What is the purpose of a Southern blot?
What is the purpose of a Southern blot?
What are the two types of antibodies?
What are the two types of antibodies?
What is the maximum specific activity of 3H?
What is the maximum specific activity of 3H?
Which type of epitope is common in fibrous proteins?
Which type of epitope is common in fibrous proteins?
What is the purpose of a Southern blot?
What is the purpose of a Southern blot?
What is the purpose of restriction fragment length polymorphism (RFLP) technique?
What is the purpose of restriction fragment length polymorphism (RFLP) technique?
What is the main difference between Southern blot and Northern blot?
What is the main difference between Southern blot and Northern blot?
Which of the following radioisotopes has the shortest half-life?
Which of the following radioisotopes has the shortest half-life?
Which of the following is NOT a potential effect of radiation sickness?
Which of the following is NOT a potential effect of radiation sickness?
What is the maximum theoretical specific activity of 3H?
What is the maximum theoretical specific activity of 3H?
What is the primary advantage of using 32P as a radioisotope for detecting DNA and RNA?
What is the primary advantage of using 32P as a radioisotope for detecting DNA and RNA?
Which of the following techniques is used to detect and localize specific DNA sequences on chromosomes?
Which of the following techniques is used to detect and localize specific DNA sequences on chromosomes?
What is the purpose of a DNA microarray?
What is the purpose of a DNA microarray?
What is the main difference between a Southern blot and a Northern blot?
What is the main difference between a Southern blot and a Northern blot?
What is the purpose of restriction fragment length polymorphism (RFLP) technique?
What is the purpose of restriction fragment length polymorphism (RFLP) technique?
Which of the following is true about restriction enzymes?
Which of the following is true about restriction enzymes?
What is the purpose of a Southern blot?
What is the purpose of a Southern blot?
What is the purpose of DNA ligase in the DNA backbone?
What is the purpose of DNA ligase in the DNA backbone?
What is the purpose of restriction fragment length polymorphism (RFLP) technique?
What is the purpose of restriction fragment length polymorphism (RFLP) technique?
Which enzyme is commonly linked to the secondary antibody in Western Blot detection?
Which enzyme is commonly linked to the secondary antibody in Western Blot detection?
What is the equation to calculate the half-life (t1/2) of a protein in a pulse-chase experiment?
What is the equation to calculate the half-life (t1/2) of a protein in a pulse-chase experiment?
What is a biosensor?
What is a biosensor?
Which of the following is NOT a class of antibodies?
Which of the following is NOT a class of antibodies?
What is the molecular weight of the light chains in an antibody?
What is the molecular weight of the light chains in an antibody?
Which enzyme cleaves an antibody into two F(ab)2 fragments?
Which enzyme cleaves an antibody into two F(ab)2 fragments?
What is the primary function of an antibody?
What is the primary function of an antibody?
Which type of epitope is common in globular proteins and helical structures?
Which type of epitope is common in globular proteins and helical structures?
What is the purpose of a Northern blot?
What is the purpose of a Northern blot?
What is the maximum specific activity of 32P?
What is the maximum specific activity of 32P?
What is the purpose of a Southern blot?
What is the purpose of a Southern blot?
Which technique is used to detect and localize specific RNA targets in cells and tissue samples?
Which technique is used to detect and localize specific RNA targets in cells and tissue samples?
What is the purpose of DNA-FISH?
What is the purpose of DNA-FISH?
What is the purpose of a Northern blot?
What is the purpose of a Northern blot?
What is the purpose of a Southern blot?
What is the purpose of a Southern blot?
Which of the following radioisotopes has the shortest half-life?
Which of the following radioisotopes has the shortest half-life?
What is the maximum specific activity of 125I?
What is the maximum specific activity of 125I?
What is the maximum specific activity of 3H?
What is the maximum specific activity of 3H?
Which of the following is NOT a safety measure when using radioactivity in the lab?
Which of the following is NOT a safety measure when using radioactivity in the lab?
Which of the following is NOT a characteristic of restriction enzymes?
Which of the following is NOT a characteristic of restriction enzymes?
What is the probability of finding a target of 7 bases?
What is the probability of finding a target of 7 bases?
Which type of restriction enzyme cuts outside of its recognition sequence?
Which type of restriction enzyme cuts outside of its recognition sequence?
What is the purpose of a Southern blot?
What is the purpose of a Southern blot?
Which of the following is NOT a commonly used mode of detection in molecular recognition?
Which of the following is NOT a commonly used mode of detection in molecular recognition?
What is the purpose of introducing a radioactive isotope into DNA, RNA, or protein for quantification purposes?
What is the purpose of introducing a radioactive isotope into DNA, RNA, or protein for quantification purposes?
Which of the following radioisotopes is commonly used to detect very small amounts of DNA, RNA, or protein?
Which of the following radioisotopes is commonly used to detect very small amounts of DNA, RNA, or protein?
What is the maximum specific activity of 32P, a commonly used radioisotope for detecting DNA and RNA?
What is the maximum specific activity of 32P, a commonly used radioisotope for detecting DNA and RNA?
Which of the following is true about the detection of proteins using chemiluminescent substrates in Western Blot?
Which of the following is true about the detection of proteins using chemiluminescent substrates in Western Blot?
What is the equation to calculate the half-life ((t_{1/2})) of a protein in a pulse-chase experiment?
What is the equation to calculate the half-life ((t_{1/2})) of a protein in a pulse-chase experiment?
Which of the following is NOT a mode of detection commonly used in molecular recognition?
Which of the following is NOT a mode of detection commonly used in molecular recognition?
What is the purpose of a biosensor?
What is the purpose of a biosensor?
Which of the following is true about a bioreceptor?
Which of the following is true about a bioreceptor?
What is the main advantage of using chemiluminescent substrates in Western Blot detection?
What is the main advantage of using chemiluminescent substrates in Western Blot detection?
