Podcast
Questions and Answers
What is one of the main challenges faced by DNA polymerases during replication?
What is one of the main challenges faced by DNA polymerases during replication?
- Dealing with energetically expensive process (correct)
- Being slow
- Not needing high energy phosphates
- Catalyzing the reaction with low accuracy
Why is accuracy important during the replication process?
Why is accuracy important during the replication process?
- To prevent mutations (correct)
- To save energy
- To increase the speed of replication
- To reduce the number of nucleotides needed
What provides energy for the polymerization reaction during DNA replication?
What provides energy for the polymerization reaction during DNA replication?
- Deoxyribonucleotides
- Ribonucleoside diphosphates
- Deoxyribonucleoside triphosphates (correct)
- Proteins
Why can't the two strands of DNA be separated along their entire length during replication?
Why can't the two strands of DNA be separated along their entire length during replication?
What is the purpose of DNA-dependent DNA polymerases during replication?
What is the purpose of DNA-dependent DNA polymerases during replication?
Why are high-energy phosphates important in deoxynucleoside triphosphates?
Why are high-energy phosphates important in deoxynucleoside triphosphates?
What would happen if mistakes occur during the replication process?
What would happen if mistakes occur during the replication process?
Why is the average rate of polymerization required to be approximately 2000 bp per second in E. coli?
Why is the average rate of polymerization required to be approximately 2000 bp per second in E. coli?
What is the average rate of polymerization required in E. coli during replication?
What is the average rate of polymerization required in E. coli during replication?
What is the consequence of any mistakes made during DNA replication?
What is the consequence of any mistakes made during DNA replication?
What is the dual purpose of deoxyribonucleoside triphosphates during replication?
What is the dual purpose of deoxyribonucleoside triphosphates during replication?
Why is accuracy important during the process of DNA replication?
Why is accuracy important during the process of DNA replication?
Why do the two strands of DNA cannot be separated along their entire length during replication of long DNA molecules?
Why do the two strands of DNA cannot be separated along their entire length during replication of long DNA molecules?
What are the high-energy phosphates in deoxynucleoside triphosphates used for?
What are the high-energy phosphates in deoxynucleoside triphosphates used for?
What is the role of DNA-dependent DNA polymerases during replication?
What is the role of DNA-dependent DNA polymerases during replication?
Why is the process of DNA replication considered energetically expensive?
Why is the process of DNA replication considered energetically expensive?
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