16 Questions
What is one of the main challenges faced by DNA polymerases during replication?
Dealing with energetically expensive process
Why is accuracy important during the replication process?
To prevent mutations
What provides energy for the polymerization reaction during DNA replication?
Deoxyribonucleoside triphosphates
Why can't the two strands of DNA be separated along their entire length during replication?
It is not energetically favorable
What is the purpose of DNA-dependent DNA polymerases during replication?
Catalyze the synthesis of new DNA strands
Why are high-energy phosphates important in deoxynucleoside triphosphates?
They provide energy for the reaction
What would happen if mistakes occur during the replication process?
Formation of mutations
Why is the average rate of polymerization required to be approximately 2000 bp per second in E. coli?
To replicate the entire genome within a specific time frame
What is the average rate of polymerization required in E. coli during replication?
Approximately 2000 bp per second
What is the consequence of any mistakes made during DNA replication?
Mutations
What is the dual purpose of deoxyribonucleoside triphosphates during replication?
Act as substrates and provide energy for polymerization reaction
Why is accuracy important during the process of DNA replication?
To prevent mutations
Why do the two strands of DNA cannot be separated along their entire length during replication of long DNA molecules?
Due to very high energy requirement
What are the high-energy phosphates in deoxynucleoside triphosphates used for?
Provide energy for polymerization reaction
What is the role of DNA-dependent DNA polymerases during replication?
Catalyze the polymerization reaction
Why is the process of DNA replication considered energetically expensive?
Due to the high energy requirement
Explore the role of enzymes in DNA replication in terms of speed and accuracy, focusing on the polymerisation process. Understand how E.coli completes replication within 18 minutes with a high rate of polymerisation. Learn about the importance of accurate replication.
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