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Enzymes in DNA Replication Speed and Accuracy

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Enzymes in DNA Replication Speed and Accuracy

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Questions and Answers

What is one of the main challenges faced by DNA polymerases during replication?

Dealing with energetically expensive process (correct)

Being slow

Not needing high energy phosphates

Catalyzing the reaction with low accuracy

Why is accuracy important during the replication process?

To prevent mutations (correct)

To save energy

To increase the speed of replication

To reduce the number of nucleotides needed

What provides energy for the polymerization reaction during DNA replication?

Deoxyribonucleotides

Ribonucleoside diphosphates

Deoxyribonucleoside triphosphates (correct)

Proteins

Why can't the two strands of DNA be separated along their entire length during replication?

It is not energetically favorable Signup and view all the answers

What is the purpose of DNA-dependent DNA polymerases during replication?

Catalyze the synthesis of new DNA strands Signup and view all the answers

Why are high-energy phosphates important in deoxynucleoside triphosphates?

They provide energy for the reaction Signup and view all the answers

What would happen if mistakes occur during the replication process?

Formation of mutations Signup and view all the answers

Why is the average rate of polymerization required to be approximately 2000 bp per second in E. coli?

To replicate the entire genome within a specific time frame Signup and view all the answers

What is the average rate of polymerization required in E. coli during replication?

Approximately 2000 bp per second Signup and view all the answers

What is the consequence of any mistakes made during DNA replication?

Mutations Signup and view all the answers

What is the dual purpose of deoxyribonucleoside triphosphates during replication?

Act as substrates and provide energy for polymerization reaction Signup and view all the answers

Why is accuracy important during the process of DNA replication?

To prevent mutations Signup and view all the answers

Why do the two strands of DNA cannot be separated along their entire length during replication of long DNA molecules?

Due to very high energy requirement Signup and view all the answers

What are the high-energy phosphates in deoxynucleoside triphosphates used for?

Provide energy for polymerization reaction Signup and view all the answers

What is the role of DNA-dependent DNA polymerases during replication?

Catalyze the polymerization reaction Signup and view all the answers

Why is the process of DNA replication considered energetically expensive?

Due to the high energy requirement Signup and view all the answers