Enzymes: Biological Catalysts Quiz

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Questions and Answers

What is a catalyst?

A catalyst is a substance that speeds up the rate of a chemical reaction, without entering the reaction itself.

Catalysts can be inorganic or organic.

True (A)

What are enzymes?

Enzymes are organic catalysts made of protein.

Most enzymes end in the suffix -ase.

<p>True (A)</p> Signup and view all the answers

How do enzymes work?

<p>Enzymes lower the activation energy needed to start a chemical reaction.</p> Signup and view all the answers

What does the lock and key model explain?

<p>The lock and key model explains how enzymes fit into their substrates.</p> Signup and view all the answers

What are the steps of enzyme action? (Select all that apply)

<p>Enzyme joins with its substrate (A), The enzyme is unchanged and can be reused (B), Enzyme and substrate form an enzyme-substrate complex (C), Enzyme action takes place (D), Enzyme and products separate (E)</p> Signup and view all the answers

The induced fit model describes a rigid enzyme that does not change shape.

<p>False (B)</p> Signup and view all the answers

What are coenzymes and cofactors?

<p>Enzyme helpers (C)</p> Signup and view all the answers

Enzymes can operate under any conditions.

<p>False (B)</p> Signup and view all the answers

Which of the following factors affect enzyme action? (Select all that apply)

<p>Concentration of enzymes (A), Temperature (B), Concentration of substrates (C), pH (D)</p> Signup and view all the answers

What is the optimal pH for pepsin, an enzyme in the stomach?

<p>2</p> Signup and view all the answers

What is the optimal pH for amylase, an enzyme found in the mouth?

<p>6.8</p> Signup and view all the answers

What is the optimal temperature for enzymes in humans?

<p>37°C</p> Signup and view all the answers

Enzymes can change shape when the temperature gets too high.

<p>True (A)</p> Signup and view all the answers

What happens to an enzyme when it is denatured?

<p>It cannot bind with its substrate.</p> Signup and view all the answers

Changes in pH can also denature enzymes.

<p>True (A)</p> Signup and view all the answers

How does the concentration of enzymes and substrates affect enzyme action?

<p>It increases the rate of enzyme action to a certain point, and then levels off.</p> Signup and view all the answers

What is the difference between a substrate and an active site?

<p>A substrate is the molecule that an enzyme acts on, while the active site is the region on the enzyme where the substrate binds.</p> Signup and view all the answers

What type of biological molecule is an enzyme considered?

<p>A protein</p> Signup and view all the answers

Why is enzyme action similar to the action of a key fitting into a lock?

<p>The enzyme's active site has a specific shape that matches the shape of its substrate.</p> Signup and view all the answers

Why are changes in pH and temperature critical to the proper functioning of an enzyme?

<p>They can denature enzymes.</p> Signup and view all the answers

Flashcards

What is a catalyst?

A substance that speeds up the rate of a chemical reaction without being consumed in the process.

What is an enzyme?

A biological catalyst, typically a protein, that speeds up specific biochemical reactions in living organisms.

What is a substrate?

The specific molecule that an enzyme acts upon. It binds to the active site of the enzyme.

What is an active site?

The region on the enzyme where the substrate binds. It has a unique shape that fits the specific substrate.

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How do enzymes affect the rate of a reaction?

Enzymes lower the activation energy of a reaction, making it easier and faster for the reaction to occur.

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What is the induced fit model?

A model that describes how enzymes bind to their specific substrates. The active site changes shape slightly to accommodate the substrate.

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What are coenzymes?

Organic molecules (like vitamins) that assist enzymes in their function. They help enzymes bind to their substrates.

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What are cofactors?

Inorganic ions (like iron) that help enzymes function. They often help with enzyme shape or charge.

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What is the optimal pH for an enzyme?

The specific pH value at which an enzyme functions most effectively.

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What is the optimal temperature for an enzyme?

The temperature at which an enzyme works best. Human enzymes work best at 37°C (98.6°F).

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What is enzyme denaturation?

A process where an enzyme loses its shape and function due to extreme temperature or pH changes. It is like 'melting' the enzyme.

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Why is denaturation problematic for enzymes?

An enzyme with a denatured structure can no longer bind to its substrate, making it inactive.

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How does concentration affect enzyme action?

The rate of enzyme action increases as the concentration of both the enzyme and the substrate increase, but eventually levels off.

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Why does the rate of enzyme action level off at high enzyme concentration?

When all the substrate is used up, the excess enzyme has nothing to work on, so the reaction rate plateaus.

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Why does the rate of enzyme action level off at high substrate concentration?

The reaction rate will initially increase as substrate concentration increases. However, if there aren't enough enzymes to act on the substrate, the rate levels off.

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What type of biological molecule is an enzyme?

A type of protein.

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Why is enzyme action similar to the lock and key model?

The active site of an enzyme has a specific shape that fits the substrate like a key in a lock.

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What is the difference between a substrate and an active site?

The substrate is the molecule the enzyme acts on. The active site is the location on the enzyme where the substrate binds.

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Why are changes in pH and temperature critical to enzyme function?

Enzymes are sensitive to changes in pH and temperature. These changes can alter their shape, potentially denaturing them and disrupting their function.

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What is catabolism?

A process that breaks down large molecules into smaller ones, often with the help of enzymes.

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What is anabolism?

A process that builds larger molecules from smaller ones, often requiring enzymes.

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What is a protease?

A specific type of enzyme that breaks down proteins into smaller peptides.

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What is an amylase?

A specific type of enzyme that breaks down carbohydrates into smaller sugars.

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What is a lipase?

A specific type of enzyme that breaks down lipids (fats) into fatty acids and glycerol.

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What is an enzyme inhibitor?

A molecule that binds to an enzyme and inhibits its activity. It can compete with the substrate for the active site.

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What is an enzyme activator?

A molecule that binds to an enzyme and increases its activity.

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What is gene regulation?

The process of turning genes 'on' or 'off' to regulate the production of enzymes.

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Study Notes

Enzymes: Biological Catalysts

  • Enzymes are biological catalysts
  • Catalysts speed up chemical reactions without being consumed
  • Most catalysts are organic, meaning they contain carbon
  • Enzymes are organic catalysts made of protein
  • Enzyme names typically end in -ase
  • Enzymes lower the activation energy of a reaction, allowing for faster reactions

How Catalysts Work

  • Catalysts speed up chemical reactions by lowering the activation energy
  • Activation energy is the minimum energy required to initiate a chemical reaction
  • Enzymes do not change the overall energy released or consumed during the reaction
  • Enzymes function by binding to substrates at the active site
  • The active site is a specific region on an enzyme

Enzyme Structure and Function

  • Enzymes typically have a larger structure than their substrates
  • Enzymes fit into their substrates like a key fits into a lock (lock-and-key model)
  • Another model is the induced fit model where the enzyme changes shape slightly upon binding with substrate
  • Enzymes participate in reactions by joining with substrates
  • The enzyme and substrate form an enzyme-substrate complex
  • Enzyme action takes place within the complex
  • The resulting products separate from the enzyme, leaving the enzyme unchanged and reusable

Enzyme Factors

  • Enzymes are sensitive to factors such as pH and temperature
  • Each enzyme has a specific pH range in which it works optimally (e.g., pepsin in the stomach works best at a low pH, amylase in the mouth works best at a neutral pH)
  • Each enzyme has an optimal temperature range, above which the enzyme's shape changes (denatures) and it loses its activity.
  • The rate of enzyme activity increases as both the enzyme and substrate concentration increase up until a certain point. Above that point, if there’s excess enzyme, or substrate, the rate won’t increase further.

Denaturation

  • Extreme temperatures or pH changes cause enzymes to denature
  • Denaturation changes the enzyme's shape
  • As a result, the enzyme can no longer bind with its substrate
  • The enzyme can no longer perform its catalytic function.

Coenzymes and Cofactors

  • Coenzymes are organic molecules that aid enzymes
  • Organic molecules typically include vitamins
  • Cofactors are inorganic molecules that assist enzymes
  • Inorganic molecules typically include metal ions such as iron

Summary Questions

  • Enzymes are proteins.
  • Enzyme action is similar to a key fitting into a lock.
  • The active site is the binding site on the enzyme.
  • Substrates are the molecules the enzyme acts on.
  • pH and temperature affect enzyme function.

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