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Questions and Answers
What is a catalyst?
What is a catalyst?
A catalyst is a substance that speeds up the rate of a chemical reaction, without entering the reaction itself.
Catalysts can be inorganic or organic.
Catalysts can be inorganic or organic.
True (A)
What are enzymes?
What are enzymes?
Enzymes are organic catalysts made of protein.
Most enzymes end in the suffix -ase.
Most enzymes end in the suffix -ase.
How do enzymes work?
How do enzymes work?
What does the lock and key model explain?
What does the lock and key model explain?
What are the steps of enzyme action? (Select all that apply)
What are the steps of enzyme action? (Select all that apply)
The induced fit model describes a rigid enzyme that does not change shape.
The induced fit model describes a rigid enzyme that does not change shape.
What are coenzymes and cofactors?
What are coenzymes and cofactors?
Enzymes can operate under any conditions.
Enzymes can operate under any conditions.
Which of the following factors affect enzyme action? (Select all that apply)
Which of the following factors affect enzyme action? (Select all that apply)
What is the optimal pH for pepsin, an enzyme in the stomach?
What is the optimal pH for pepsin, an enzyme in the stomach?
What is the optimal pH for amylase, an enzyme found in the mouth?
What is the optimal pH for amylase, an enzyme found in the mouth?
What is the optimal temperature for enzymes in humans?
What is the optimal temperature for enzymes in humans?
Enzymes can change shape when the temperature gets too high.
Enzymes can change shape when the temperature gets too high.
What happens to an enzyme when it is denatured?
What happens to an enzyme when it is denatured?
Changes in pH can also denature enzymes.
Changes in pH can also denature enzymes.
How does the concentration of enzymes and substrates affect enzyme action?
How does the concentration of enzymes and substrates affect enzyme action?
What is the difference between a substrate and an active site?
What is the difference between a substrate and an active site?
What type of biological molecule is an enzyme considered?
What type of biological molecule is an enzyme considered?
Why is enzyme action similar to the action of a key fitting into a lock?
Why is enzyme action similar to the action of a key fitting into a lock?
Why are changes in pH and temperature critical to the proper functioning of an enzyme?
Why are changes in pH and temperature critical to the proper functioning of an enzyme?
Flashcards
What is a catalyst?
What is a catalyst?
A substance that speeds up the rate of a chemical reaction without being consumed in the process.
What is an enzyme?
What is an enzyme?
A biological catalyst, typically a protein, that speeds up specific biochemical reactions in living organisms.
What is a substrate?
What is a substrate?
The specific molecule that an enzyme acts upon. It binds to the active site of the enzyme.
What is an active site?
What is an active site?
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How do enzymes affect the rate of a reaction?
How do enzymes affect the rate of a reaction?
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What is the induced fit model?
What is the induced fit model?
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What are coenzymes?
What are coenzymes?
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What are cofactors?
What are cofactors?
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What is the optimal pH for an enzyme?
What is the optimal pH for an enzyme?
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What is the optimal temperature for an enzyme?
What is the optimal temperature for an enzyme?
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What is enzyme denaturation?
What is enzyme denaturation?
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Why is denaturation problematic for enzymes?
Why is denaturation problematic for enzymes?
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How does concentration affect enzyme action?
How does concentration affect enzyme action?
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Why does the rate of enzyme action level off at high enzyme concentration?
Why does the rate of enzyme action level off at high enzyme concentration?
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Why does the rate of enzyme action level off at high substrate concentration?
Why does the rate of enzyme action level off at high substrate concentration?
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What type of biological molecule is an enzyme?
What type of biological molecule is an enzyme?
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Why is enzyme action similar to the lock and key model?
Why is enzyme action similar to the lock and key model?
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What is the difference between a substrate and an active site?
What is the difference between a substrate and an active site?
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Why are changes in pH and temperature critical to enzyme function?
Why are changes in pH and temperature critical to enzyme function?
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What is catabolism?
What is catabolism?
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What is anabolism?
What is anabolism?
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What is a protease?
What is a protease?
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What is an amylase?
What is an amylase?
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What is a lipase?
What is a lipase?
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What is an enzyme inhibitor?
What is an enzyme inhibitor?
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What is an enzyme activator?
What is an enzyme activator?
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What is gene regulation?
What is gene regulation?
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Study Notes
Enzymes: Biological Catalysts
- Enzymes are biological catalysts
- Catalysts speed up chemical reactions without being consumed
- Most catalysts are organic, meaning they contain carbon
- Enzymes are organic catalysts made of protein
- Enzyme names typically end in -ase
- Enzymes lower the activation energy of a reaction, allowing for faster reactions
How Catalysts Work
- Catalysts speed up chemical reactions by lowering the activation energy
- Activation energy is the minimum energy required to initiate a chemical reaction
- Enzymes do not change the overall energy released or consumed during the reaction
- Enzymes function by binding to substrates at the active site
- The active site is a specific region on an enzyme
Enzyme Structure and Function
- Enzymes typically have a larger structure than their substrates
- Enzymes fit into their substrates like a key fits into a lock (lock-and-key model)
- Another model is the induced fit model where the enzyme changes shape slightly upon binding with substrate
- Enzymes participate in reactions by joining with substrates
- The enzyme and substrate form an enzyme-substrate complex
- Enzyme action takes place within the complex
- The resulting products separate from the enzyme, leaving the enzyme unchanged and reusable
Enzyme Factors
- Enzymes are sensitive to factors such as pH and temperature
- Each enzyme has a specific pH range in which it works optimally (e.g., pepsin in the stomach works best at a low pH, amylase in the mouth works best at a neutral pH)
- Each enzyme has an optimal temperature range, above which the enzyme's shape changes (denatures) and it loses its activity.
- The rate of enzyme activity increases as both the enzyme and substrate concentration increase up until a certain point. Above that point, if there’s excess enzyme, or substrate, the rate won’t increase further.
Denaturation
- Extreme temperatures or pH changes cause enzymes to denature
- Denaturation changes the enzyme's shape
- As a result, the enzyme can no longer bind with its substrate
- The enzyme can no longer perform its catalytic function.
Coenzymes and Cofactors
- Coenzymes are organic molecules that aid enzymes
- Organic molecules typically include vitamins
- Cofactors are inorganic molecules that assist enzymes
- Inorganic molecules typically include metal ions such as iron
Summary Questions
- Enzymes are proteins.
- Enzyme action is similar to a key fitting into a lock.
- The active site is the binding site on the enzyme.
- Substrates are the molecules the enzyme acts on.
- pH and temperature affect enzyme function.
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