Enzymes and Their Functions

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Questions and Answers

Which is the isoenzyme designated as CK3?

  • CK1 or BB
  • CK2 or MB
  • CK3 or MM (correct)
  • ALL CK isoenzymes are the same

What is the optimum pH for the forward reaction of creatine kinase?

  • 6.8
  • 9.0 (correct)
  • 8.0
  • 7.4

Which condition is NOT associated with increased levels of CK?

  • Alcoholic Myopathy
  • Hypothyroidism
  • Chronic Kidney Disease (correct)
  • Myocardial Infarction

Which enzyme is primarily associated with hepatic disorders?

<p>ALT (B)</p> Signup and view all the answers

In cases of acute pancreatitis, which substances show an increase?

<p>Amylase and Lipase (A)</p> Signup and view all the answers

What is a primary function of phosphatases?

<p>To hydrolyze organic phosphate compounds (A)</p> Signup and view all the answers

Which of the following diseases is NOT associated with increased alkaline phosphatase (ALP) activity?

<p>Diabetes Mellitus (D)</p> Signup and view all the answers

What is the highest elevation of acid phosphatase (ACP) levels associated with?

<p>Prostatic carcinoma metastasis (A)</p> Signup and view all the answers

Which condition is least likely to cause elevated amylase levels?

<p>Cholecystitis (D)</p> Signup and view all the answers

In which of the following isoenzymes is lipase primarily found?

<p>Pancreas (B)</p> Signup and view all the answers

What clinical significance is associated with elevated lipase levels?

<p>Pancreatic duct obstruction (C)</p> Signup and view all the answers

Which enzyme has isoenzymes that include aspartate aminotransferase (AST)?

<p>Aminotransferases (C)</p> Signup and view all the answers

Which of the following conditions is typically indicated by elevated alkaline phosphatase levels?

<p>Paget's disease (B)</p> Signup and view all the answers

What is the primary substrate for the enzymatic activity of alanine aminotransferase (ALT)?

<p>Alanine (C)</p> Signup and view all the answers

Which clinical condition is associated with increased levels of aspartate aminotransferase (AST)?

<p>Myocardial infarction (B)</p> Signup and view all the answers

What role does lactate dehydrogenase (LDH) play in the body?

<p>Catalyzes the interconversion of lactic acid and pyruvic acid (C)</p> Signup and view all the answers

Which enzyme is primarily involved in the diagnosis of hepatotoxicity?

<p>Alanine aminotransferase (A)</p> Signup and view all the answers

In which condition might one expect to see moderately to markedly elevated levels of lactate dehydrogenase (LDH)?

<p>Hemolytic anemia (A)</p> Signup and view all the answers

Which isoenzyme of lactate dehydrogenase is associated with the heart muscle?

<p>LDH1 (D)</p> Signup and view all the answers

What is the main function of creatine kinase in muscle cells?

<p>Catalyzing the interconversion of creatine and ATP (C)</p> Signup and view all the answers

Which factor might lead to decreased levels of alanine aminotransferase (ALT)?

<p>Pregnancy (A)</p> Signup and view all the answers

What distinguishes enzymes from other biological molecules?

<p>They speed up chemical reactions without being consumed. (A)</p> Signup and view all the answers

Which suffix is commonly added to the names of enzymes derived from their substrates?

<p>ase (B)</p> Signup and view all the answers

What role do coenzymes play in enzymatic reactions?

<p>They provide essential organic molecules for reactions. (D)</p> Signup and view all the answers

Which classification of enzymes is responsible for transferring chemical groups?

<p>Transferases (C)</p> Signup and view all the answers

What is the primary function of hydrolases?

<p>To split a bond by the addition of water. (C)</p> Signup and view all the answers

What are zymogens also known as?

<p>Proenzymes (A)</p> Signup and view all the answers

Which of the following is a specific example of an oxidoreductase?

<p>Lactate dehydrogenase (D)</p> Signup and view all the answers

What is a common characteristic of isomerases?

<p>They catalyze the rearrangement of molecules. (A)</p> Signup and view all the answers

Flashcards

Fructose Diphosphate Aldolase (ALD)

Enzyme that catalyzes the splitting of D-fructose diphosphate into D-glyceraldehyde phosphate and dihydroxy acetone phosphate. This enzyme has multiple isoenzymes.

CK-MB

The CK isoenzyme marker specific for myocardial infarction. Elevates rapidly after an MI.

What are enzymes?

Enzymes are biological catalysts, meaning they speed up chemical reactions within living organisms without being consumed in the process.

Alanine Transaminase (ALT)

This enzyme is an indicator of liver damage and is elevated in cases like hepatitis, cirrhosis and liver cancer.

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How are enzymes named?

Enzymes are named based on the substance they act upon (substrate) by adding the suffix 'ase'. For example, protease breaks down proteins.

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Alkaline Phosphatase (ALP)

An enzyme associated with both liver and bone disorders. Elevated levels can be seen in conditions such as hepatitis, obstructive jaundice, and bone diseases including Paget's disease.

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Amylase

This enzyme is a primary indicator of acute pancreatitis. Elevated levels can be found in acute or chronic pancreatitis as well as other conditions like gallstones, trauma, and alcohol abuse.

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What do oxidoreductases do?

Oxidoreductases catalyze oxidation-reduction reactions, which involve the transfer of electrons. They are essential for cellular respiration and energy production.

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What do transferases do?

Transferases move chemical groups from one molecule to another. They are vital for biosynthesis and metabolism, helping build and break down molecules.

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What do hydrolases do?

Hydrolases break down bonds within molecules using water. Important for digestion and nutrient breakdown.

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What do lyases do?

Lyases break chemical bonds without adding water. They create double bonds, crucial for synthesis of molecules.

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What do isomerases do?

Isomerases change the structure of a molecule, creating different forms of the same compound. Essential for rearranging molecules for different purposes.

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What is a "haloenzyme"?

A 'haloenzyme' is a complete, active enzyme consisting of a protein part (apoenzyme) and a non-protein component (cofactor).

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Phosphatases

An enzyme group that breaks down organic phosphates, playing a crucial role in various metabolic processes.

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Acid Phosphatase (ACP)

A type of enzyme, often found in prostate cells, that can be a biomarker for prostate cancer. Elevated levels might suggest prostate issues.

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Alanine Aminotransferase (ALT)

An enzyme that catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate, forming glutamate and pyruvate.

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Aspartate Aminotransferase (AST)

A transferase with enzymatic activity similar to ALT, catalyzing transfer of an amino group from aspartate to alpha-ketoglutarate forming glutamate and oxaloacetate.

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Lipase

An enzyme involved in the breakdown of fats into fatty acids and glycerol. High levels can indicate pancreatic problems.

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Aminotransferases

A group of enzymes that transfer amino groups from one molecule to another, playing a key role in amino acid metabolism.

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Lactate Dehydrogenase (LDH)

An enzyme found in various tissues like the heart, liver, skeletal muscle, and erythrocytes. It catalyzes the interconversion of lactic acid and pyruvic acid.

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Creatine Kinase (CK)

An enzyme that promotes the conversion of creatine and ATP to creatine phosphate and ADP, providing energy for muscle contraction.

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Aspartate Aminotransferase (AST)

An aminotransferase enzyme found in many tissues, including the heart, liver, and skeletal muscle. Elevated levels can indicate damage to these tissues.

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Alanine Aminotransferase (ALT)

An aminotransferase enzyme primarily found in the liver. Elevated levels can indicate liver damage or disease.

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What does elevated ALT suggest?

An elevated ALT level in the blood can indicate liver damage, particularly in cases of acute or chronic viral hepatitis.

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What does elevated AST suggest?

Increased AST levels are often associated with myocardial infarction (heart attack), but also with other conditions like pulmonary infarction, pericarditis, and muscle damage.

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What does elevated LDH suggest?

Elevated LDH levels point to various conditions like myocardial infarction, liver disease, muscle trauma, and hemolytic disease.

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What does elevated CK suggest?

Increased CK levels are frequently associated with muscle damage, especially in cases of myocardial infarction and strenuous activity.

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Study Notes

Enzymes

  • Remarkable molecules in living organisms, crucial for biological processes
  • Aid in breaking down food, converting nutrients to energy, building and repairing tissues
  • Enable chemical reactions vital for life
  • Catalysts, speeding up reactions without being consumed

Enzyme Classification by Function

  • Oxidoreductases: Involved in oxidation-reduction reactions, transfer of electrons

    • Oxidases: Removal or addition of electrons
    • Dehydrogenases: Specific examples include LDH (Lactate Dehydrogenase), MDH (Malate Dehydrogenase), and others
  • Transferases: Transfer chemical groups between molecules

    • Specific examples: AST (Aspartate transaminase), ALT (Alanine transaminase), CK (Creatine Kinase), etc.
  • Hydrolases: Split bonds by adding water

    • Specific examples: ACP (Acid phosphatase), ALP (Alkaline phosphatase), LPS (Lipase), peptidases, PTS, etc.
  • Lyases: Remove groups, leaving double bonds

    • Specific examples: Aldolases, glutamate decarboxylase, pyruvate decarboxylase, etc.
  • Isomerases: Rearrange atoms within a molecule

    • Examples: Glucose phosphate isomerase, Ribose phosphate isomerase and others
  • Ligases: Join two molecules together

    • Not explicitly listed in the provided text

Enzyme Nomenclature

  • Named according to their substrate and the suffix "ase" (e.g., protease, lipase)
  • Classified by the type of reaction they catalyze (e.g., kinase, dehydrogenase)
  • Classified numerically by the Enzyme Commission
  • Apoenzymes: Protein component of an enzyme
  • Cofactors: Non-protein molecules essential for enzyme function
  • Holoenzymes: Complete enzymes with both protein and non-protein components
  • Isoenzymes: Different forms of an enzyme with similar functions
  • Metal-containing Enzymes: Enzymes that utilize metal ions for activity
  • Proenzymes (Zymogens): Inactive precursors of enzymes that need to be activated before function

Clinical Significance of Enzymes

  • Enzyme levels in blood can be diagnostic indicators of certain diseases or conditions.
  • Elevated levels may indicate tissue damage or disease (e.g., myocardial infarction, pancreatitis, hepatitis)
  • Measured to monitor disease progression or treatment effectiveness

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