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Questions and Answers
What is the primary function of enzymes in biological systems?
What is the primary function of enzymes in biological systems?
- To store genetic information
- To slow down chemical reactions
- To speed up chemical reactions (correct)
- To provide structural support
Enzymes are always made of only one polypeptide chain.
Enzymes are always made of only one polypeptide chain.
False (B)
What is the specific region on an enzyme where the substrate binds?
What is the specific region on an enzyme where the substrate binds?
active site
The _____ model describes how the enzyme and substrate change shape upon binding.
The _____ model describes how the enzyme and substrate change shape upon binding.
Match the following terms with their corresponding descriptions:
Match the following terms with their corresponding descriptions:
What happens to an enzyme when it undergoes denaturation?
What happens to an enzyme when it undergoes denaturation?
Enzymes are primarily composed of globular proteins.
Enzymes are primarily composed of globular proteins.
What is the bare minimum of energy required for a chemical reaction to occur?
What is the bare minimum of energy required for a chemical reaction to occur?
Enzymes ______ the activation energy of biochemical reactions.
Enzymes ______ the activation energy of biochemical reactions.
What is the primary factor determining the rate of enzyme activity?
What is the primary factor determining the rate of enzyme activity?
A higher substrate concentration always leads to a faster reaction rate for an enzyme.
A higher substrate concentration always leads to a faster reaction rate for an enzyme.
Match the following terms with their definitions:
Match the following terms with their definitions:
Explain how enzymes facilitate chemical reactions.
Explain how enzymes facilitate chemical reactions.
The collision frequency always increases as substrate concentration increases.
The collision frequency always increases as substrate concentration increases.
What happens to the reaction rate when the enzyme's active sites are saturated with substrate molecules?
What happens to the reaction rate when the enzyme's active sites are saturated with substrate molecules?
The ______ of an enzyme is the specific condition (temperature or pH) where it functions most effectively.
The ______ of an enzyme is the specific condition (temperature or pH) where it functions most effectively.
Which of the following factors influences enzyme activity?
Which of the following factors influences enzyme activity?
According to collision theory, why is it necessary for substrate molecules to collide with enzyme active sites?
According to collision theory, why is it necessary for substrate molecules to collide with enzyme active sites?
Adding more enzyme to a reaction mixture always increases the reaction rate.
Adding more enzyme to a reaction mixture always increases the reaction rate.
What is the most likely outcome of increasing the temperature significantly above an enzyme's optimum temperature?
What is the most likely outcome of increasing the temperature significantly above an enzyme's optimum temperature?
Proteins are polymers made by joining amino acids through peptide bonds, which are formed through a hydrolysis reaction.
Proteins are polymers made by joining amino acids through peptide bonds, which are formed through a hydrolysis reaction.
What is the primary structure of a protein determined by?
What is the primary structure of a protein determined by?
The process of breaking down large molecules into smaller ones is called ______.
The process of breaking down large molecules into smaller ones is called ______.
Explain how temperature can affect the structure and function of a protein.
Explain how temperature can affect the structure and function of a protein.
What is the primary role of enzymes in metabolic reactions?
What is the primary role of enzymes in metabolic reactions?
Increasing the temperature always increases the rate of an enzyme-catalyzed reaction.
Increasing the temperature always increases the rate of an enzyme-catalyzed reaction.
Describe the relationship between substrate concentration and the rate of an enzyme-catalyzed reaction.
Describe the relationship between substrate concentration and the rate of an enzyme-catalyzed reaction.
The process of breaking down complex molecules into simpler ones with the addition of water is called ______.
The process of breaking down complex molecules into simpler ones with the addition of water is called ______.
Match the following enzyme types with their respective roles in metabolic processes:
Match the following enzyme types with their respective roles in metabolic processes:
Anabolic reactions are energy-releasing processes.
Anabolic reactions are energy-releasing processes.
Which of the following is an example of an anabolic process?
Which of the following is an example of an anabolic process?
Which of the following is NOT a type of interaction that contributes to the tertiary structure of a protein?
Which of the following is NOT a type of interaction that contributes to the tertiary structure of a protein?
The optimum pH for most enzymes is 7, which is a neutral pH.
The optimum pH for most enzymes is 7, which is a neutral pH.
What are the two main types of secondary protein structures?
What are the two main types of secondary protein structures?
The process of unfolding or breaking down a protein is called ______.
The process of unfolding or breaking down a protein is called ______.
Match the level of protein structure with its corresponding description:
Match the level of protein structure with its corresponding description:
Which level of protein structure is primarily responsible for the overall shape and stability of the protein?
Which level of protein structure is primarily responsible for the overall shape and stability of the protein?
Disulfide bridges are a type of ionic bond that contributes to the tertiary structure of proteins.
Disulfide bridges are a type of ionic bond that contributes to the tertiary structure of proteins.
What is the significance of the primary structure of a protein?
What is the significance of the primary structure of a protein?
Flashcards
Denaturation
Denaturation
The disruption of an enzyme's structure due to external factors like heat or pH.
Active Site
Active Site
The specific region of an enzyme where substrate binding occurs.
Globular Protein
Globular Protein
Proteins that are folded into a rounded shape, important for enzyme function.
Enzyme Function
Enzyme Function
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Activation Energy
Activation Energy
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Enzyme-Substrate Complex
Enzyme-Substrate Complex
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Collision Frequency
Collision Frequency
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Reaction Rate
Reaction Rate
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Enzymes
Enzymes
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Induced-Fit Model
Induced-Fit Model
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Substrate Specificity
Substrate Specificity
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Catalytic Bonds
Catalytic Bonds
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Polypeptide Chains
Polypeptide Chains
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Chemical Reaction Speed
Chemical Reaction Speed
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Enzyme Saturation
Enzyme Saturation
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Optimal Temperature
Optimal Temperature
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Optimal pH
Optimal pH
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Catalyst
Catalyst
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Collision Theory
Collision Theory
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Anabolism
Anabolism
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Catabolism
Catabolism
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Condensation
Condensation
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Hydrolysis
Hydrolysis
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Amino Acids
Amino Acids
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Peptide Bond
Peptide Bond
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Protein Denaturation
Protein Denaturation
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Protein Structure Levels
Protein Structure Levels
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Primary Structure
Primary Structure
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Secondary Structure
Secondary Structure
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Tertiary Structure
Tertiary Structure
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Quaternary Structure
Quaternary Structure
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Enzyme stability
Enzyme stability
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Molecular Motion
Molecular Motion
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Substrate Concentration
Substrate Concentration
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Saturation
Saturation
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Condensation Reaction
Condensation Reaction
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Study Notes
Enzyme Structure and Function
- Enzymes are globular proteins that act as catalysts, speeding up chemical reactions in living organisms.
- The active site of an enzyme binds specifically to its substrate.
- The induced-fit model shows how the enzyme and substrate change shape upon binding for better function
- Enzyme activity can be affected or destroyed by factors like heat and pH changes, resulting in denaturation and loss of function.
- Enzymes are proteins made of amino acids joined by peptide bonds forming polypeptide chains that fold into complex three-dimensional shapes.
- Enzymes help accelerate reactions by lowering the activation energy
- Enzymes are involved in every metabolic process in living organisms.
- Each enzyme has a unique active site with a specific shape and chemical properties that facilitate precise binding with its substrate.
- The active site of an enzyme can be compared to a lock (enzyme) and key (substrate).
- Enzymes work efficiently at specific temperature and pH ranges.
Enzyme Activity and Catalysis
- Enzymes lower the activation energy for chemical reactions.
- Activation energy is the minimum energy required to initiate a reaction.
- Enzymes speed up reactions by providing an alternative reaction pathway with a lower activation energy.
- The rate of enzyme activity is influenced by the frequency of collisions between the enzyme and substrate molecules.
- Enzyme activity increases as substrate concentration increases, up to the point maximum capacity is reached, where the enzyme is saturated causing no further increase in reaction rate.
- Enzymes work optimally at specific temperature and pH ranges.
Enzyme Roles in Metabolism
- Metabolism is the web of all enzyme-catalyzed reactions in a cell or organism.
- Anabolism involves synthesizing complex molecules from simpler molecules requiring energy.
- Catabolism involves breaking down complex molecules into simpler molecules, releasing energy.
- Enzymes play a critical role in both anabolic and catabolic pathways such as glycolysis and photosynthesis.
Protein Structure and Environmental Factors
- Proteins are made from amino acids linked by peptide bonds.
- The primary structure of a protein is its linear sequence of amino acids.
- Secondary structure involves folding of the polypeptide chain into structures like alpha-helices and beta-sheets.
- Tertiary structure is the three-dimensional shape of the protein.
- Quaternary structure refers to the arrangement of multiple polypeptide chains in proteins with multiple subunits.
- Factors affecting protein structure include pH, temperature, and other environmental conditions, such as high salt concentrations.
- Extreme changes in conditions like high temperature and changes in pH can cause a disruption in protein shape (denaturation) leading to a loss of function.
- Enzymes operate optimally at certain temperature and pH ranges.
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