Enzyme Stabilization Methods
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Questions and Answers

What is a major advantage of immobilizing enzymes over stabilizing them through solvent stabilization?

  • Immobilized enzymes are more expensive to produce
  • Immobilized enzymes can be reused or used continuously (correct)
  • Immobilized enzymes have lower enzymatic activity
  • Immobilized enzymes are more susceptible to denaturation

Which of the following immobilization methods involves the formation of covalent bonds between the enzyme molecules?

  • Adsorption
  • Cross-linking (correct)
  • Covalent binding
  • Entrapment

What is a major disadvantage of solvent stabilization methods?

  • They can cause enzyme denaturation
  • They can only be used for certain enzymes
  • The enzymes remain soluble and cannot be reused (correct)
  • They can be expensive

What type of ions have been found to stabilize the tertiary structure of proteases and α-amylases?

<p>Calcium ions (D)</p> Signup and view all the answers

Which immobilization method involves the physical adsorption of enzyme protein on the surface of water-insoluble carriers?

<p>Adsorption (B)</p> Signup and view all the answers

What is the most common reagent used for cross-linking?

<p>Glutaraldehyde (B)</p> Signup and view all the answers

What occurs to enzymes when solvents are used at high concentrations?

<p>Denaturation occurs (B)</p> Signup and view all the answers

What is a method of enzyme stabilization that involves adding a substrate?

<p>Substrate stabilization (B)</p> Signup and view all the answers

What is a type of enzyme stabilization that involves forming a covalent bond between the enzyme and a support material?

<p>Covalent binding (B)</p> Signup and view all the answers

What happens to an enzyme when it is denatured?

<p>It loses its function (B)</p> Signup and view all the answers

What is the term for the process of attaching an enzyme to a support material to increase its stability?

<p>Immobilization (B)</p> Signup and view all the answers

What is a method of enzyme stabilization that involves adding a cation?

<p>Cation stabilization (C)</p> Signup and view all the answers

What is the main disadvantage of using alkanes in the production of SCP?

<p>They are not easily soluble (B)</p> Signup and view all the answers

What is a characteristic of microbial insecticides?

<p>Their toxicity to non-target animals and humans is extremely low (A)</p> Signup and view all the answers

What is the function of biofertilizers?

<p>To enrich the nutrient quality of the soil (D)</p> Signup and view all the answers

What type of insecticides are Bacillus thuringiensis and Bacillus popilliae?

<p>Microbial insecticides (B)</p> Signup and view all the answers

What is the name of the toxin produced by Bacillus thuringiensis?

<p>α-exotoxin (D)</p> Signup and view all the answers

What type of relationship do microorganisms in biofertilizers establish with plants?

<p>Symbiotic relationship (C)</p> Signup and view all the answers

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