Enzyme Stabilization Methods

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What is a major advantage of immobilizing enzymes over stabilizing them through solvent stabilization?

Immobilized enzymes are more expensive to produce

Immobilized enzymes can be reused or used continuously (correct)

Immobilized enzymes have lower enzymatic activity

Immobilized enzymes are more susceptible to denaturation

Which of the following immobilization methods involves the formation of covalent bonds between the enzyme molecules?

Adsorption

Cross-linking (correct)

Covalent binding

Entrapment

What is a major disadvantage of solvent stabilization methods?

They can cause enzyme denaturation

They can only be used for certain enzymes

The enzymes remain soluble and cannot be reused (correct)

They can be expensive

What type of ions have been found to stabilize the tertiary structure of proteases and α-amylases?

Calcium ions Signup and view all the answers

Which immobilization method involves the physical adsorption of enzyme protein on the surface of water-insoluble carriers?

Adsorption Signup and view all the answers

What is the most common reagent used for cross-linking?

Glutaraldehyde Signup and view all the answers

What occurs to enzymes when solvents are used at high concentrations?

Denaturation occurs Signup and view all the answers

What is a method of enzyme stabilization that involves adding a substrate?

Substrate stabilization Signup and view all the answers

What is a type of enzyme stabilization that involves forming a covalent bond between the enzyme and a support material?

Covalent binding Signup and view all the answers

What happens to an enzyme when it is denatured?

It loses its function Signup and view all the answers

What is the term for the process of attaching an enzyme to a support material to increase its stability?

Immobilization Signup and view all the answers

What is a method of enzyme stabilization that involves adding a cation?

Cation stabilization Signup and view all the answers

What is the main disadvantage of using alkanes in the production of SCP?

They are not easily soluble Signup and view all the answers

What is a characteristic of microbial insecticides?

Their toxicity to non-target animals and humans is extremely low Signup and view all the answers

What is the function of biofertilizers?

To enrich the nutrient quality of the soil Signup and view all the answers

What type of insecticides are Bacillus thuringiensis and Bacillus popilliae?

Microbial insecticides Signup and view all the answers

What is the name of the toxin produced by Bacillus thuringiensis?

α-exotoxin Signup and view all the answers

What type of relationship do microorganisms in biofertilizers establish with plants?

Symbiotic relationship Signup and view all the answers