Enzyme Stabilization Methods
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Questions and Answers

What is a major advantage of immobilizing enzymes over stabilizing them through solvent stabilization?

  • Immobilized enzymes are more expensive to produce
  • Immobilized enzymes can be reused or used continuously (correct)
  • Immobilized enzymes have lower enzymatic activity
  • Immobilized enzymes are more susceptible to denaturation
  • Which of the following immobilization methods involves the formation of covalent bonds between the enzyme molecules?

  • Adsorption
  • Cross-linking (correct)
  • Covalent binding
  • Entrapment
  • What is a major disadvantage of solvent stabilization methods?

  • They can cause enzyme denaturation
  • They can only be used for certain enzymes
  • The enzymes remain soluble and cannot be reused (correct)
  • They can be expensive
  • What type of ions have been found to stabilize the tertiary structure of proteases and α-amylases?

    <p>Calcium ions</p> Signup and view all the answers

    Which immobilization method involves the physical adsorption of enzyme protein on the surface of water-insoluble carriers?

    <p>Adsorption</p> Signup and view all the answers

    What is the most common reagent used for cross-linking?

    <p>Glutaraldehyde</p> Signup and view all the answers

    What occurs to enzymes when solvents are used at high concentrations?

    <p>Denaturation occurs</p> Signup and view all the answers

    What is a method of enzyme stabilization that involves adding a substrate?

    <p>Substrate stabilization</p> Signup and view all the answers

    What is a type of enzyme stabilization that involves forming a covalent bond between the enzyme and a support material?

    <p>Covalent binding</p> Signup and view all the answers

    What happens to an enzyme when it is denatured?

    <p>It loses its function</p> Signup and view all the answers

    What is the term for the process of attaching an enzyme to a support material to increase its stability?

    <p>Immobilization</p> Signup and view all the answers

    What is a method of enzyme stabilization that involves adding a cation?

    <p>Cation stabilization</p> Signup and view all the answers

    What is the main disadvantage of using alkanes in the production of SCP?

    <p>They are not easily soluble</p> Signup and view all the answers

    What is a characteristic of microbial insecticides?

    <p>Their toxicity to non-target animals and humans is extremely low</p> Signup and view all the answers

    What is the function of biofertilizers?

    <p>To enrich the nutrient quality of the soil</p> Signup and view all the answers

    What type of insecticides are Bacillus thuringiensis and Bacillus popilliae?

    <p>Microbial insecticides</p> Signup and view all the answers

    What is the name of the toxin produced by Bacillus thuringiensis?

    <p>α-exotoxin</p> Signup and view all the answers

    What type of relationship do microorganisms in biofertilizers establish with plants?

    <p>Symbiotic relationship</p> Signup and view all the answers

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