12 Questions
What is the main purpose of the method described in the text?
To quantify patient specimens
What is the sensitivity of the method compared to RIA?
Equally sensitive
What is the advantage of this method over RIA?
Does not require special equipment or radioactive labels
What is the purpose of reacting the enzyme-linked material with the patient's specimen?
To quantify antigens or antibodies
What happens after any free primary antibody is washed away in the indirect ELISA method?
The enzyme-linked material is washed away
What is the purpose of adding secondary antibody to human IgG labeled with an enzyme in the indirect ELISA method?
To bind to the primary antibody and detect its presence
What is the main function of the specialized spectrophotometric plate readers in the indirect ELISA method?
To measure the absorbance of all of the wells
What is the specific purpose of adding a substrate for the enzyme in the indirect ELISA method?
To measure the colored reaction product that forms
In the Indirect ELISA method, what is the role of the substrate for the enzyme?
It amplifies the color reaction
What is the sensitivity of the Indirect ELISA method compared to RIA?
Higher sensitivity than RIA
What is the highest dilution of serum that gives a positive reaction in the Indirect ELISA method?
1:1000
What is the advantage of the Indirect ELISA method over RIA?
Higher specificity
Test your knowledge of the Enzyme-Linked Immunosorbent Assay (ELISA) method for quantitating antigens or antibodies in patient specimens. Understand the principles and procedures of this technique.
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