Enzyme Inhibition
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Questions and Answers

What type of inhibitor binds to an allosteric site on the protein?

  • Un-competitive inhibitor
  • Competitive inhibitor
  • Non-competitive inhibitor (correct)
  • Irreversible inhibitor

    • What is the effect of a non-competitive inhibitor on the Km and Vmax of an enzyme?

  • Km remains the same, Vmax decreases (correct)
  • Km increases, Vmax remains the same
  • Km increases, Vmax decreases
  • Km decreases, Vmax increases

    • What type of inhibitor binds only to the enzyme-substrate complex?

  • Un-competitive inhibitor (correct)
  • Non-competitive inhibitor
  • Irreversible inhibitor
  • Competitive inhibitor

    • What is the effect of a competitive inhibitor on the Km and Vmax of an enzyme?

    <p>Km increases, Vmax remains the same</p> Signup and view all the answers

    What is irreversible inhibition?

    <p>Covalent binding of an inhibitor to the active site or an allosteric site</p> Signup and view all the answers

    What is the effect of an un-competitive inhibitor on the Km and Vmax of an enzyme?

    <p>Km increases, Vmax decreases</p> Signup and view all the answers

    What type of inhibitor increases Km, but does not affect Vmax?

    <p>Competitive inhibitor</p> Signup and view all the answers

    What is the effect of a non-competitive inhibitor on the Lineweaver-Burk plot?

    <p>Decreases the slope, increases the y-intercept</p> Signup and view all the answers

    What is the name of the method used to linearize the non-linear Michaelis-Menten equation?

    <p>Lineweaver-Burk method</p> Signup and view all the answers

    What is the effect of a competitive inhibitor on the Km of an enzyme?

    <p>It increases the Km</p> Signup and view all the answers

    What is the name of the model proposed to describe enzyme kinetics in 1913?

    <p>Michaelis-Menten model</p> Signup and view all the answers

    What is the term for the regulation of enzyme activity by gene expression?

    <p>Gene expression regulation</p> Signup and view all the answers

    What is the name of the authors of the biochemistry textbook 'Biochemistry'?

    <p>L.Stryer</p> Signup and view all the answers

    What is the term for the phenomenon where multiple substrate binding sites are present on an enzyme?

    <p>Cooperativity</p> Signup and view all the answers

    What is the term for the type of inhibition where the inhibitor binds to an allosteric site on the enzyme?

    <p>Allosteric inhibition</p> Signup and view all the answers

    What is the term for the type of inhibition where the inhibitor binds to the active site of the enzyme?

    <p>Competitive inhibition</p> Signup and view all the answers

    Which subunit combination represents the LDH isozyme specific to the skeletal muscle and liver?

    <p>MMMM</p> Signup and view all the answers

    Which disease is associated with increased levels of all five isozymes of LDH?

    <p>Leukemia</p> Signup and view all the answers

    What does ELISA stand for?

    <p>Enzyme-linked immunosorbent assay</p> Signup and view all the answers

    Which enzyme is used as a biomarker for acute pancreatitis?

    <p>Amylase</p> Signup and view all the answers

    What is the primary location of the HHHH LDH isozyme?

    <p>Heart and RBCs</p> Signup and view all the answers

    Which enzyme is a diagnostic marker for viral hepatitis?

    <p>SGPT</p> Signup and view all the answers

    What is the biomedical importance of γ-glutamyl transferase?

    <p>Liver disease especially biliary obstruction</p> Signup and view all the answers

    What is the principle behind ELISA?

    <p>Linking an enzyme to an antibody to detect antigen</p> Signup and view all the answers

    Which factor is NOT listed as affecting enzyme activity?

    <p>Enzyme color</p> Signup and view all the answers

    Why is enzyme expression not useful for rapid adaptation?

    <p>It involves the synthesis of new RNA transcripts and proteins</p> Signup and view all the answers

    Which condition causes most enzymes to denature?

    <p>Temperature above ~50 °C</p> Signup and view all the answers

    Which statement best describes feedback inhibition?

    <p>A product of a reaction inhibits the enzyme's activity</p> Signup and view all the answers

    What happens when effectors bind to the enzyme at the substrate (active) site?

    <p>They act as competitive inhibitors</p> Signup and view all the answers

    What happens to the reaction rate with a 10 °C temperature rise?

    <p>It doubles</p> Signup and view all the answers

    How does a change in pH potentially affect enzyme activity?

    <p>By altering the enzyme's conformation or the substrate's protonation state</p> Signup and view all the answers

    Which type of inhibition is described when the enzyme's activity is regulated by the molecule that binds elsewhere than the active site?

    <p>Allosteric regulation</p> Signup and view all the answers

    Where do allosteric effectors bind to an enzyme?

    <p>At a site different from the substrate binding pocket</p> Signup and view all the answers

    What happens to KM during K-type regulation?

    <p>KM decreases</p> Signup and view all the answers

    What is the effect on Vmax during V-type regulation?

    <p>Vmax increases</p> Signup and view all the answers

    What is a characteristic of isoenzymes?

    <p>They have genetically determined differences in amino acid sequence</p> Signup and view all the answers

    Which isoenzyme is associated with cardiac muscle?

    <p>CK2</p> Signup and view all the answers

    What can happen during mixed forms of allosteric regulation?

    <p>Both KM and Vmax can be affected</p> Signup and view all the answers

    During enzyme inhibition, which type of inhibitor does not affect the binding of the substrate?

    <p>Non-competitive inhibitor</p> Signup and view all the answers

    What is an allosteric inhibitor's effect on enzyme activity?

    <p>Decreases enzyme activity</p> Signup and view all the answers

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