Enzyme Inhibition and Regulation

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Questions and Answers

What is a primary function of selectively permeable membranes?

  • Prevent any movement of water.
  • Always require energy for transport.
  • Allow all molecules to pass freely.
  • Separate internal and external environments. (correct)

Which component of the membrane is primarily responsible for its fluidity?

  • Protein composition.
  • Sterols.
  • Amount of water present.
  • Phospholipid structure. (correct)

How does facilitated diffusion function in the context of membrane transport?

  • Moves molecules against their concentration gradient.
  • Requires ATP for the movement of substances.
  • Involves transport proteins to move substances down their concentration gradient. (correct)
  • Is limited to only small uncharged molecules.

What role do unsaturated fatty acids play in membrane characteristics?

<p>Increase membrane fluidity due to kinks. (B)</p> Signup and view all the answers

What is osmosis?

<p>Diffusion of water across a selectively permeable membrane. (B)</p> Signup and view all the answers

Which statement correctly describes the role of sterols in cellular membranes?

<p>They decrease membrane fluidity at high temperatures. (D)</p> Signup and view all the answers

Which of the following molecules would likely have the most difficulty crossing a selectively permeable membrane?

<p>Glucose. (D)</p> Signup and view all the answers

What distinguishes primary active transport from secondary active transport?

<p>Primary moves substances against gradients, while secondary relies on energy from primary transport. (D)</p> Signup and view all the answers

What is the primary function of exocytosis?

<p>Secretion of substances (D)</p> Signup and view all the answers

Which type of endocytosis is specifically involved in the uptake of large particles?

<p>Phagocytosis (D)</p> Signup and view all the answers

What role do kinases play in signaling pathways?

<p>They add phosphates (D)</p> Signup and view all the answers

What physiological response is NOT associated with adrenaline?

<p>Decreased energy levels (D)</p> Signup and view all the answers

In a redox reaction, which substance is characterized as the electron donor?

<p>Oxidized substance (C)</p> Signup and view all the answers

Which of the following best describes receptor-mediated endocytosis?

<p>Selective uptake involving receptors (D)</p> Signup and view all the answers

What characterizes competitive inhibition of an enzyme?

<p>The inhibitor prevents substrate binding by occupying the active site. (D)</p> Signup and view all the answers

Which statement about oxidation is true?

<p>It is coupled with reduction (B)</p> Signup and view all the answers

What is the primary purpose of feedback inhibition in enzymatic pathways?

<p>To conserve cellular resources by regulating pathway activity. (D)</p> Signup and view all the answers

What is the role of electron carriers like NAD+ in cellular processes?

<p>Transporting electrons and protons (B)</p> Signup and view all the answers

How does pH affect enzyme activity according to the provided information?

<p>pH changes can affect the charged groups in the enzyme's amino acids. (B)</p> Signup and view all the answers

What typically happens to the reaction rates of enzymes below their optimal temperature?

<p>Reaction rates increase as the temperature approaches the optimal level. (C)</p> Signup and view all the answers

What occurs to heat-sensitive enzymes at high temperatures?

<p>They may denature, losing their structure and function. (D)</p> Signup and view all the answers

Which statement accurately describes noncompetitive inhibition?

<p>The enzymatic activity decreases without preventing substrate binding. (C)</p> Signup and view all the answers

What is a typical pH value for the optimal activity of intracellular enzymes?

<p>Near neutral pH (~7) (C)</p> Signup and view all the answers

During feedback inhibition, how does the product of a pathway typically regulate the reaction?

<p>By binding to an allosteric site of the first enzyme in the pathway. (B)</p> Signup and view all the answers

What is produced during the citric acid cycle for each acetyl group that enters?

<p>2 CO2, 1 ATP, 3 NADH, 2 FADH2 (D)</p> Signup and view all the answers

Which statement accurately describes the role of NAD+ and FAD in the citric acid cycle?

<p>They are oxidized to donate electrons and are reduced to form NADH and FADH2. (D)</p> Signup and view all the answers

Which phase of cellular respiration occurs in the cytosol?

<p>Glycolysis (D)</p> Signup and view all the answers

What is the main purpose of fermentation in eukaryotic cells?

<p>To produce ethanol or lactic acid and regenerate NAD+. (D)</p> Signup and view all the answers

How many ATP molecules are produced at the end of the citric acid cycle for two molecules of acetyl-CoA?

<p>2 ATP (B)</p> Signup and view all the answers

Which of the following accurately describes the flow of energy in cellular respiration?

<p>Energy is generated during glycolysis, citric acid cycle, and oxidative phosphorylation. (A)</p> Signup and view all the answers

In which process is pyruvate converted into lactic acid or ethanol?

<p>Anaerobic Fermentation (A)</p> Signup and view all the answers

What is the final electron acceptor in the electron transport chain during oxidative phosphorylation?

<p>O2 (D)</p> Signup and view all the answers

What role does phosphofructokinase play in the metabolic pathway?

<p>It catalyzes the second ATP-consuming step of the pathway. (C)</p> Signup and view all the answers

What happens during anaerobic respiration?

<p>The terminal electron acceptor is not oxygen. (A)</p> Signup and view all the answers

What two types of fermentation are identified in eukaryotic cells under low oxygen conditions?

<p>Lactate fermentation and alcohol fermentation. (D)</p> Signup and view all the answers

What is the main danger of oxygen for aerobic organisms?

<p>It can form reactive oxygen species (ROS). (D)</p> Signup and view all the answers

Which enzymes are part of the antioxidant defense system?

<p>Superoxide dismutase and catalase. (C)</p> Signup and view all the answers

What are chlorophyll b and carotenoids classified as in relation to chlorophyll a?

<p>Accessory pigments. (D)</p> Signup and view all the answers

What was the key finding from Engelmann's experiment?

<p>Algae produce the most oxygen under blue, violet, and red light. (B)</p> Signup and view all the answers

During the light reactions of photosynthesis, what is produced?

<p>ATP and NADPH. (D)</p> Signup and view all the answers

Which reaction center chlorophyll molecule is found in Photosystem II?

<p>P680. (B)</p> Signup and view all the answers

What is the function of the photosynthetic electron transport chain?

<p>It facilitates electron flow from water to NADP+. (C)</p> Signup and view all the answers

What must happen for light energy to be utilized by a molecule?

<p>The light must be absorbed by the molecule. (A)</p> Signup and view all the answers

Which of the following describes the structure that allows pigments to efficiently absorb light?

<p>A conjugated system of carbon atoms with alternating single and double bonds. (D)</p> Signup and view all the answers

What is released as a by-product during the light reactions of photosynthesis?

<p>Oxygen (A)</p> Signup and view all the answers

Which group of organisms can produce organic compounds using light energy?

<p>Photoautotrophs (B)</p> Signup and view all the answers

What happens to an excited-state electron after it absorbs energy?

<p>It can move to a higher energy state or be transferred to another molecule. (C)</p> Signup and view all the answers

Which statement accurately reflects the overall reaction of photosynthesis?

<p>CO2 + H2O → O2 + Organic molecules (D)</p> Signup and view all the answers

Where in eukaryotic cells does photosynthesis primarily occur?

<p>Chloroplasts (D)</p> Signup and view all the answers

How do autotrophs differ from heterotrophs?

<p>Autotrophs synthesize organic molecules from inorganic sources, while heterotrophs rely on organic molecules from other organisms. (A)</p> Signup and view all the answers

What is the role of ATP and NADPH in the Calvin cycle?

<p>They provide energy and reducing power for synthesizing carbohydrates. (D)</p> Signup and view all the answers

Which of the following processes is not a fate of an excited-state electron?

<p>Being permanently lost to the surrounding environment. (D)</p> Signup and view all the answers

Flashcards

Competitive Inhibition

An inhibitor directly competes with the substrate for the enzyme's active site, blocking substrate binding.

Noncompetitive Inhibition

An inhibitor binds to a site other than the active site (allosteric site), changing the enzyme's shape and reducing its activity.

Feedback Inhibition

The product of an enzyme-catalyzed pathway inhibits the earlier steps of the pathway.

Enzyme Optimal Temperature

The temperature at which an enzyme works most efficiently.

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Enzyme Optimal pH

The pH value at which an enzyme is most active.

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Enzyme Denaturation

The process in which high temperatures cause an enzyme to lose its structural integrity, affecting activity.

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Heat-sensitive Enzymes

Enzymes that lose their activity at higher temperatures.

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Selectively Permeable Membranes

Membranes that control what passes through them, allowing some molecules and ions to cross but not others.

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Fluid Mosaic Model

The model describing cell membranes as a fluid bilayer with proteins embedded and floating.

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Passive Transport

Movement of molecules across a membrane without energy input, driven by concentration gradients.

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Simple Diffusion

Molecules moving directly across a membrane from high to low concentration.

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Facilitated Diffusion

Molecules moving across a membrane with the help of transport proteins, still down their concentration gradient.

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Osmosis

Diffusion of water across a selectively permeable membrane, from a lower solute to higher solute concentration.

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Active Transport

Movement of molecules against their concentration gradient requiring energy (ATP).

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Primary Active Transport

Active transport where the transport protein directly uses ATP to move substances.

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Secondary Active Transport

Active transport that uses energy stored in an electrochemical gradient created by a primary active transporter.

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Membrane Fluidity

The ability of lipid molecules to move within the cell membrane.

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Amphipathic

Having both hydrophilic and hydrophobic parts.

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Phospholipids

Major component of cell membranes, composed of hydrophilic head and hydrophobic tails.

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Lipid Bilayer

Two layers of lipids arranged with tails facing each other.

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Redox Reaction (NAD+/NADH)

A chemical reaction involving the transfer of electrons, where one molecule is oxidized and another is reduced, exemplified by the interconversion of NAD+ and NADH.

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Redox Reaction (FAD/FADH2)

A chemical reaction involving the transfer of electrons, where one molecule is oxidized and another is reduced, exemplified by the interconversion of FAD and FADH2.

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Cellular Respiration

The process where glucose is broken down to release energy in the form of ATP, using oxygen, producing carbon dioxide and water as byproducts.

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Glycolysis

The first stage of cellular respiration, breaking down glucose into pyruvate in the cytoplasm.

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Citric Acid Cycle

A series of reactions that oxidizes acetyl groups (derived from pyruvate), producing ATP, NADH, and FADH2.

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Oxidative Phosphorylation

The final stage of cellular respiration where NADH and FADH2 donate electrons to the electron transport chain, generating ATP.

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Fermentation

A metabolic process that produces ATP from glucose without the need for oxygen; produces products like lactic acid or ethanol.

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Lactate Fermentation

Type of fermentation in animals and bacteria where the NADH produced during glycolysis transfers electrons to pyruvate, forming lactate and regenerating NAD+.

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Alcoholic Fermentation

Type of fermentation in plants and fungi where NADH from glycolysis transfers electrons to pyruvate, forming ethanol and regenerating NAD+.

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Electron Transport Chain

A series of protein complexes in the inner mitochondrial membrane that use electrons from NADH and FADH2 to generate a proton gradient, which is used to produce ATP.

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Exocytosis

Vesicles fuse with the plasma membrane to secrete substances.

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Endocytosis

Internalization of external materials via vesicles.

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Pinocytosis

Non-specific uptake of fluids (cell drinking).

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Phagocytosis

Uptake of large particles (cell eating).

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Receptor-Mediated Endocytosis

Specific uptake via receptor-clathrin interactions.

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Cell Signaling Reception

Signal molecule binds to receptor.

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Cell Signaling Transduction

Signal relayed via intracellular cascades.

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Cell Signaling Response

Cellular activity altered.

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Cell Signaling Termination

Signal turned off after response.

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Signal Amplification

One signal activates multiple downstream molecules.

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Kinases

Add phosphates to molecules.

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Phosphatases

Remove phosphates from molecules.

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Oxidation

Partial or full loss of electrons from a substance.

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Reduction

Partial or full gain of electrons by a substance.

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Redox Reactions

Oxidation and reduction occur simultaneously.

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Electron Carriers (NAD+)

Accepts 2 electrons and 1 proton (becomes NADH).

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Electron Carriers (FAD)

Accepts 2 electrons and 2 protons (becomes FADH2).

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Anaerobic Respiration

Respiration that does not utilize oxygen as the terminal electron acceptor.

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Fermentation

A metabolic pathway used in respiration when oxygen is absent.

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Lactate Fermentation

One type of fermentation, producing lactate as a byproduct.

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Alcohol Fermentation

Another type of fermentation, producing alcohol as a byproduct.

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Reactive Oxygen Species (ROS)

Harmful oxidizing agents like superoxide and hydrogen peroxide.

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Antioxidant Defence System

The body's protection against reactive oxygen species.

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Chlorophylls

Major photosynthetic pigments in plants and some bacteria.

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Accessory Pigments

Pigments that assist chlorophyll in capturing light energy.

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Absorption Spectrum

Graph showing light absorbed by a pigment at different wavelengths.

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Action Spectrum

Graph showing the efficiency of different wavelengths in driving photosynthesis.

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Engelmann's Experiment

Experiment demonstrating the wavelengths most effective for photosynthesis.

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Light Reactions

First stage of photosynthesis, capturing light energy.

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Calvin Cycle

Second phase of photosynthesis, using energy to create sugars.

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Antenna Complex

Part of photosystem that absorbs light and transfers energy.

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Reaction Center

Specific chlorophyll molecules where light energy is converted into chemical energy.

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Photosystem II (PSII)

Photosystem responsible for extracting electrons from water.

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Photosystem I (PSI)

Photosystem involved in reducing NADP+ to NADPH.

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Photosynthetic Electron Transport Chain

Chain that links PSII and PSI, moving electrons.

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Light Absorption

When photons of light hit an object, they can be reflected, transmitted, or absorbed.

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Energy Transfer

For light to be used as energy, it has to be absorbed. This energy is transferred to an electron in a molecule.

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Excited State

Absorption of light energy causes an electron in a molecule to move from its original level to a higher energy level, an excited state.

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Pigments

Molecules that absorb photons of specific wavelengths. Their structure affects what wavelengths they absorb.

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Conjugated System

A chain of carbon atoms linked by alternating single and double bonds. This is needed to absorb photons for pigments including chlorophyll.

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Pigment Color

The color of a pigment is determined by the wavelengths of light it doesn't absorb.

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Chlorophyll

A photosynthetic pigment that is highly efficient at absorbing visible light.

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Electromagnetic Spectrum

Arrangement of different types of electromagnetic radiation, from high-energy gamma rays to low-energy radio waves, visible light is one form.

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Visible Light Spectrum

The range of wavelengths of light visible to humans, from violet (400 nm) to red (700 nm).

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Fluorescence

When an electron returns to its ground state, releasing energy as a longer wavelength of light.

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Inductive Resonance

Energy transfer between neighboring pigment molecules.

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Photoreduction

Transfer of excited-state electron to a nearby electron-accepting molecule.

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Photosynthesis

Process using light energy to convert CO2 into organic molecules, releasing oxygen as a byproduct.

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Autotrophs

Organisms that produce their own food from inorganic sources.

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Photoautotrophs

Autotrophs that use light as their energy source for photosynthesis.

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Heterotrophs

Organisms that obtain energy by consuming other organisms.

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Light Reactions

The first stage of photosynthesis, converting light energy into ATP and NADPH, producing oxygen as a byproduct.

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Calvin Cycle

The second stage of photosynthesis, using ATP and NADPH from the light reactions to fix carbon dioxide into sugars.

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Chloroplasts

Organelles in eukaryotic cells where photosynthesis takes place.

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Study Notes

Enzyme Inhibition

  • Enzymes are proteins that catalyze reactions
  • Inhibitors are non-substrate molecules that bind to an enzyme, decreasing its activity
  • Competitive inhibition: The inhibitor competes with the substrate for the active site
  • Noncompetitive inhibition: The inhibitor binds to a site other than the active site (allosteric site), altering the enzyme's structure

Feedback Inhibition

  • The product of an enzyme-catalyzed pathway acts as a regulator for the reaction, typically by binding to an allosteric site.
  • This is a form of allosteric regulation.
  • Purpose: Conserves cellular resources

Effects of Temperature and pH on Enzyme Activity

  • Each enzyme has specific optimal temperature and pH values for maximum efficiency
  • Temperature: Typically matches the physiological temperature of the organism. For intracellular enzymes, near neutral pH (~7). For extracellular enzymes, the pH optimum may vary..
  • Effects of Deviations from Optimum:
    • pH Changes: Affects the charged groups in the amino acids of the enzyme, which can alter enzyme structure and reduce activity
    • Temperature Changes: Below optimal temperatures, reaction rates increase as temperature rises towards the ideal temperature. Above the optimum temperature, high temperatures cause denaturation of the enzyme (loss of structural integrity), decreasing reaction rates.

Specific Temperature Effects on Enzyme Activity

  • Example: Enzymes controlling melanin production are heat-sensitive.

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